Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   ACJEHT_RS04365 Genome accession   NZ_CP174243
Coordinates   982703..983302 (-) Length   199 a.a.
NCBI ID   WP_011215096.1    Uniprot ID   -
Organism   Legionella pneumophila strain SPF582     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 977703..988302
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACJEHT_RS04345 (ACJEHT_04345) aroB 977953..979062 (-) 1110 WP_408073318.1 3-dehydroquinate synthase -
  ACJEHT_RS04350 (ACJEHT_04350) aroK 979049..979576 (-) 528 WP_010946667.1 shikimate kinase AroK -
  ACJEHT_RS04355 (ACJEHT_04355) pilQ 980022..982118 (-) 2097 WP_110539734.1 type IV pilus secretin PilQ Machinery gene
  ACJEHT_RS04360 (ACJEHT_04360) pilP 982122..982706 (-) 585 WP_027266404.1 pilus assembly protein PilP Machinery gene
  ACJEHT_RS04365 (ACJEHT_04365) pilO 982703..983302 (-) 600 WP_011215096.1 type 4a pilus biogenesis protein PilO Machinery gene
  ACJEHT_RS04370 (ACJEHT_04370) pilN 983316..983864 (-) 549 WP_010946663.1 PilN domain-containing protein Machinery gene
  ACJEHT_RS04375 (ACJEHT_04375) pilM 983868..984932 (-) 1065 WP_011215094.1 type IV pilus assembly protein PilM Machinery gene
  ACJEHT_RS04380 (ACJEHT_04380) ravI 985349..986356 (+) 1008 WP_011215093.1 Dot/Icm T4SS effector RavI -

Sequence

Protein


Download         Length: 199 a.a.        Molecular weight: 22482.18 Da        Isoelectric Point: 5.3369

>NTDB_id=1076427 ACJEHT_RS04365 WP_011215096.1 982703..983302(-) (pilO) [Legionella pneumophila strain SPF582]
MDTVNLSELTLENVGQWPMPVKIGVILGISILIIGLGYWLIVKPNFEQLEIQEAQEVALKTEFEKKQHQAANLQAYRNQL
QLMNERFGTMLKQLPAKNEMPGLLEEISKTGIASGLKFELFAPQPEVVHDFYVELPIKITIVGSYMQLARFLSRVAEMNR
IVTLHEFSVEGVSSGDGKTVSQDELVMAITAKIYRYRAQ

Nucleotide


Download         Length: 600 bp        

>NTDB_id=1076427 ACJEHT_RS04365 WP_011215096.1 982703..983302(-) (pilO) [Legionella pneumophila strain SPF582]
ATGGACACGGTCAATTTAAGTGAATTAACTCTGGAGAATGTCGGGCAGTGGCCAATGCCGGTTAAAATCGGAGTCATTTT
AGGTATCAGTATACTGATAATTGGATTAGGATATTGGCTTATTGTTAAGCCCAATTTTGAGCAATTGGAAATACAGGAAG
CCCAGGAAGTTGCTTTGAAAACAGAGTTTGAAAAAAAGCAACATCAGGCAGCAAATCTACAAGCTTATCGTAATCAGTTA
CAGTTAATGAATGAACGCTTTGGGACTATGCTAAAACAACTCCCGGCGAAAAATGAAATGCCAGGGCTTTTGGAGGAAAT
ATCTAAAACGGGGATTGCATCGGGTTTGAAATTTGAATTGTTTGCACCACAACCTGAAGTAGTGCATGATTTTTATGTGG
AGTTACCTATAAAAATTACGATTGTCGGATCCTATATGCAATTGGCAAGATTTTTAAGTCGTGTGGCTGAAATGAACAGA
ATAGTGACTTTGCATGAATTTAGTGTTGAGGGGGTGTCATCCGGGGATGGTAAAACGGTCTCTCAGGATGAATTGGTGAT
GGCTATCACTGCGAAAATATACAGATATCGGGCACAATGA

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(49-197)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Legionella pneumophila strain ERS1305867

98.995

100

0.99
  comO Acinetobacter baylyi ADP1

39.894

94.472

0.377
  pilO Acinetobacter baumannii D1279779

37.696

95.98

0.362

Multiple sequence alignment