Detailed information NTG1076219

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: ACJEHH_RS04360
• Genome accession: NZ_CP174238
• Coordinates: 982644..983708 (-)
• Length: 354 a.a.
• NCBI ID: WP_011215094.1
• Uniprot ID: Q5WXY1
• Organism: Legionella pneumophila strain SPF587
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 977644..988708

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACJEHH_RS04335 (ACJEHH_04335)	aroK	977825..978352 (-)	528	WP_010946667.1	shikimate kinase AroK	-
ACJEHH_RS04340 (ACJEHH_04340)	pilQ	978798..980894 (-)	2097	WP_110539734.1	type IV pilus secretin PilQ	Machinery gene
ACJEHH_RS04345 (ACJEHH_04345)	pilP	980898..981482 (-)	585	WP_027266404.1	pilus assembly protein PilP	Machinery gene
ACJEHH_RS04350 (ACJEHH_04350)	pilO	981479..982078 (-)	600	WP_011215096.1	type 4a pilus biogenesis protein PilO	Machinery gene
ACJEHH_RS04355 (ACJEHH_04355)	pilN	982092..982640 (-)	549	WP_010946663.1	PilN domain-containing protein	Machinery gene
ACJEHH_RS04360 (ACJEHH_04360)	pilM	982644..983708 (-)	1065	WP_011215094.1	type IV pilus assembly protein PilM	Machinery gene
ACJEHH_RS04365 (ACJEHH_04365)	ravI	984125..985132 (+)	1008	WP_011215093.1	Dot/Icm T4SS effector RavI	-
ACJEHH_RS04370 (ACJEHH_04370)	-	985284..987668 (+)	2385	WP_408073314.1	penicillin-binding protein 1A	-

Sequence

Protein

• Length: 354 a.a.
• Molecular weight: 38760.78 Da
• Isoelectric Point: 5.0367

>NTDB_id=1076219 ACJEHH_RS04360 WP_011215094.1 982644..983708(-) (pilM) [Legionella pneumophila strain SPF587]
MLKLLKPKHRSILGIDISSSAVKILEISGSGEDICVEGYGREEIPASALEGNMIKDVDVVANCIKKLVERLKFTSKNVAL
AVPDSAVISKVVQINEGLTDLEMEELVVIEADKYIPYPIDEINLDFEVLGHSAKSSSLLDVLIVASRAENVNTRVEAAHR
AGLEPKVVDVESYAVERSVQQIAQELPASGQDKIVAIVDIGANYTHLFVLHGMRLIFSREEKFGGMQLVEAIAEHYHMNL
EQAIALKNQGKLPEDYESAVLEPFKDMILLQIKRTLQFFYSTSQHGFVDHILLAGGMARQPGLAALIQEQLGVTTTVANP
LSHMSFGKKVNLDVINSDAPTLMVACGLALRHVE

Nucleotide

• Length: 1065 bp

>NTDB_id=1076219 ACJEHH_RS04360 WP_011215094.1 982644..983708(-) (pilM) [Legionella pneumophila strain SPF587]
GTGCTCAAATTGCTTAAACCTAAGCATCGTTCAATTCTTGGCATTGACATCAGTTCTTCCGCAGTGAAAATTCTGGAAAT
ATCAGGTTCCGGAGAAGACATTTGTGTTGAAGGATATGGTCGTGAAGAAATACCTGCCTCTGCCCTGGAAGGAAATATGA
TCAAGGATGTCGATGTAGTAGCGAATTGTATCAAAAAATTAGTAGAAAGATTGAAGTTTACATCTAAAAATGTGGCATTG
GCTGTACCTGATTCGGCTGTTATCAGTAAAGTAGTGCAAATTAATGAAGGGTTAACTGACCTTGAAATGGAAGAGCTTGT
CGTTATTGAGGCGGATAAGTACATTCCTTATCCCATAGATGAAATAAATCTGGATTTTGAAGTGTTAGGCCATTCAGCCA
AGAGCTCATCACTGCTTGATGTTTTAATCGTGGCATCAAGGGCAGAAAACGTTAATACCCGTGTTGAAGCAGCCCATAGA
GCAGGGCTGGAACCGAAAGTTGTTGACGTTGAGTCTTATGCAGTAGAGCGTTCAGTACAACAAATTGCGCAAGAATTACC
GGCAAGTGGGCAAGACAAAATTGTTGCAATAGTAGATATTGGAGCCAATTATACCCACTTATTTGTTTTGCATGGTATGC
GACTAATCTTTTCAAGAGAAGAAAAATTTGGAGGTATGCAACTTGTTGAAGCTATTGCAGAACACTATCATATGAATTTG
GAGCAGGCGATTGCCCTAAAAAATCAGGGTAAACTACCGGAGGACTACGAATCTGCTGTATTAGAACCTTTTAAGGATAT
GATTCTTTTGCAGATCAAACGTACTTTGCAGTTTTTTTATTCAACCAGTCAACATGGATTTGTAGACCATATTTTATTGG
CGGGAGGAATGGCAAGGCAACCAGGATTGGCAGCGCTTATTCAAGAACAGTTAGGAGTGACTACTACAGTGGCAAATCCA
CTATCCCATATGTCTTTTGGTAAAAAAGTGAATTTGGATGTGATTAACAGTGATGCGCCTACACTGATGGTGGCATGTGG
GTTGGCTTTGAGGCATGTCGAGTAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-352)

3D structure

• AlphaFold DB: Q5WXY1

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Legionella pneumophila strain ERS1305867	100	100	1
comM	Acinetobacter nosocomialis M2	42.373	100	0.424
pilM	Acinetobacter baumannii D1279779	42.09	100	0.421
comM	Acinetobacter baylyi ADP1	40.678	100	0.407