Detailed information NTG1076123

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: ACJEIB_RS05260
• Genome accession: NZ_CP174236
• Coordinates: 1148039..1149103 (+)
• Length: 354 a.a.
• NCBI ID: WP_011215094.1
• Uniprot ID: Q5WXY1
• Organism: Legionella pneumophila strain SPF589
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1143039..1154103

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACJEIB_RS05250 (ACJEIB_05250)	-	1144079..1146463 (-)	2385	WP_408073314.1	penicillin-binding protein 1A	-
ACJEIB_RS05255 (ACJEIB_05255)	ravI	1146615..1147622 (-)	1008	WP_011215093.1	Dot/Icm T4SS effector RavI	-
ACJEIB_RS05260 (ACJEIB_05260)	pilM	1148039..1149103 (+)	1065	WP_011215094.1	type IV pilus assembly protein PilM	Machinery gene
ACJEIB_RS05265 (ACJEIB_05265)	pilN	1149107..1149655 (+)	549	WP_010946663.1	PilN domain-containing protein	Machinery gene
ACJEIB_RS05270 (ACJEIB_05270)	pilO	1149669..1150268 (+)	600	WP_011215096.1	type 4a pilus biogenesis protein PilO	Machinery gene
ACJEIB_RS05275 (ACJEIB_05275)	pilP	1150265..1150849 (+)	585	WP_027266404.1	pilus assembly protein PilP	Machinery gene
ACJEIB_RS05280 (ACJEIB_05280)	pilQ	1150853..1152949 (+)	2097	WP_110539734.1	type IV pilus secretin PilQ	Machinery gene
ACJEIB_RS05285 (ACJEIB_05285)	aroK	1153395..1153922 (+)	528	WP_010946667.1	shikimate kinase AroK	-

Sequence

Protein

• Length: 354 a.a.
• Molecular weight: 38760.78 Da
• Isoelectric Point: 5.0367

>NTDB_id=1076123 ACJEIB_RS05260 WP_011215094.1 1148039..1149103(+) (pilM) [Legionella pneumophila strain SPF589]
MLKLLKPKHRSILGIDISSSAVKILEISGSGEDICVEGYGREEIPASALEGNMIKDVDVVANCIKKLVERLKFTSKNVAL
AVPDSAVISKVVQINEGLTDLEMEELVVIEADKYIPYPIDEINLDFEVLGHSAKSSSLLDVLIVASRAENVNTRVEAAHR
AGLEPKVVDVESYAVERSVQQIAQELPASGQDKIVAIVDIGANYTHLFVLHGMRLIFSREEKFGGMQLVEAIAEHYHMNL
EQAIALKNQGKLPEDYESAVLEPFKDMILLQIKRTLQFFYSTSQHGFVDHILLAGGMARQPGLAALIQEQLGVTTTVANP
LSHMSFGKKVNLDVINSDAPTLMVACGLALRHVE

Nucleotide

• Length: 1065 bp

>NTDB_id=1076123 ACJEIB_RS05260 WP_011215094.1 1148039..1149103(+) (pilM) [Legionella pneumophila strain SPF589]
GTGCTCAAATTGCTTAAACCTAAGCATCGTTCAATTCTTGGCATTGACATCAGTTCTTCCGCAGTGAAAATTCTGGAAAT
ATCAGGTTCCGGAGAAGACATTTGTGTTGAAGGATATGGTCGTGAAGAAATACCTGCCTCTGCCCTGGAAGGAAATATGA
TCAAGGATGTCGATGTAGTAGCGAATTGTATCAAAAAATTAGTAGAAAGATTGAAGTTTACATCTAAAAATGTGGCATTG
GCTGTACCTGATTCGGCTGTTATCAGTAAAGTAGTGCAAATTAATGAAGGGTTAACTGACCTTGAAATGGAAGAGCTTGT
CGTTATTGAGGCGGATAAGTACATTCCTTATCCCATAGATGAAATAAATCTGGATTTTGAAGTGTTAGGCCATTCAGCCA
AGAGCTCATCACTGCTTGATGTTTTAATCGTGGCATCAAGGGCAGAAAACGTTAATACCCGTGTTGAAGCAGCCCATAGA
GCAGGGCTGGAACCGAAAGTTGTTGACGTTGAGTCTTATGCAGTAGAGCGTTCAGTACAACAAATTGCGCAAGAATTACC
GGCAAGTGGGCAAGACAAAATTGTTGCAATAGTAGATATTGGAGCCAATTATACCCACTTATTTGTTTTGCATGGTATGC
GACTAATCTTTTCAAGAGAAGAAAAATTTGGAGGTATGCAACTTGTTGAAGCTATTGCAGAACACTATCATATGAATTTG
GAGCAGGCGATTGCCCTAAAAAATCAGGGTAAACTACCGGAGGACTACGAATCTGCTGTATTAGAACCTTTTAAGGATAT
GATTCTTTTGCAGATCAAACGTACTTTGCAGTTTTTTTATTCAACCAGTCAACATGGATTTGTAGACCATATTTTATTGG
CGGGAGGAATGGCAAGGCAACCAGGATTGGCAGCGCTTATTCAAGAACAGTTAGGAGTGACTACTACAGTGGCAAATCCA
CTATCCCATATGTCTTTTGGTAAAAAAGTGAATTTGGATGTGATTAACAGTGATGCGCCTACACTGATGGTGGCATGTGG
GTTGGCTTTGAGGCATGTCGAGTAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-352)

3D structure

• AlphaFold DB: Q5WXY1

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Legionella pneumophila strain ERS1305867	100	100	1
comM	Acinetobacter nosocomialis M2	42.373	100	0.424
pilM	Acinetobacter baumannii D1279779	42.09	100	0.421
comM	Acinetobacter baylyi ADP1	40.678	100	0.407