Detailed information NTG1076013

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: ACJEHF_RS05770
• Genome accession: NZ_CP174233
• Coordinates: 1294598..1295662 (-)
• Length: 354 a.a.
• NCBI ID: WP_010946662.1
• Uniprot ID: Q5ZX06
• Organism: Legionella pneumophila strain SPF601
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1289598..1300662

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACJEHF_RS05745 (ACJEHF_05745)	aroK	1289776..1290303 (-)	528	WP_010946667.1	shikimate kinase AroK	-
ACJEHF_RS05750 (ACJEHF_05750)	pilQ	1290749..1292845 (-)	2097	WP_010946666.1	type IV pilus secretin PilQ	Machinery gene
ACJEHF_RS05755 (ACJEHF_05755)	pilP	1292849..1293436 (-)	588	WP_010946665.1	pilus assembly protein PilP	Machinery gene
ACJEHF_RS05760 (ACJEHF_05760)	pilO	1293433..1294032 (-)	600	WP_014841277.1	type 4a pilus biogenesis protein PilO	Machinery gene
ACJEHF_RS05765 (ACJEHF_05765)	pilN	1294046..1294594 (-)	549	WP_010946663.1	PilN domain-containing protein	Machinery gene
ACJEHF_RS05770 (ACJEHF_05770)	pilM	1294598..1295662 (-)	1065	WP_010946662.1	type IV pilus assembly protein PilM	Machinery gene
ACJEHF_RS05775 (ACJEHF_05775)	ravI	1296079..1297089 (+)	1011	WP_010946661.1	Dot/Icm T4SS effector RavI	-
ACJEHF_RS05780 (ACJEHF_05780)	-	1297241..1299625 (+)	2385	WP_010946660.1	penicillin-binding protein 1A	-

Sequence

Protein

• Length: 354 a.a.
• Molecular weight: 38732.71 Da
• Isoelectric Point: 5.0367

>NTDB_id=1076013 ACJEHF_RS05770 WP_010946662.1 1294598..1295662(-) (pilM) [Legionella pneumophila strain SPF601]
MLKLLKPKHRSILGIDISSSAVKILEISGSGEDICVEGYGREEIPASALEGNIIKDVDVVANCIKKLVERLKFTSKNVAL
AVPDSAVISKVVQINEGLTDLEMEELVVIEADKYIPYPIDEINLDFEVLGHSAKSSSLLDVLIVASRAENVNTRVEAAHR
AGLEPKVVDVESYAVERSVQQIAQELPASGQDKIVAIVDIGANYTHLFVLHGMRLIFSREEKFGGMQLVEAIAEHYHMNL
EQAIALKNQGKLPEDYESAVLEPFKDMILLQIKRTLQFFYSTSQHGFVDHILLAGGMARQSGLAALIQEQLGVTTTVANP
LSHMSFGKKVNLDVINSDAPTLMVACGLALRHVE

Nucleotide

• Length: 1065 bp

>NTDB_id=1076013 ACJEHF_RS05770 WP_010946662.1 1294598..1295662(-) (pilM) [Legionella pneumophila strain SPF601]
GTGCTCAAATTGCTTAAACCTAAGCATCGTTCAATTCTTGGCATTGACATCAGTTCTTCCGCAGTGAAAATCCTGGAAAT
ATCAGGTTCCGGAGAGGACATTTGTGTTGAAGGATATGGTCGCGAAGAAATACCTGCCTCTGCCTTAGAAGGAAATATAA
TTAAGGATGTCGATGTAGTAGCGAATTGTATCAAAAAATTAGTAGAAAGATTGAAGTTTACATCTAAAAATGTGGCATTG
GCTGTACCTGATTCGGCTGTTATCAGTAAAGTAGTGCAAATTAATGAAGGGTTAACTGACCTTGAAATGGAAGAGCTTGT
CGTTATTGAGGCGGATAAGTACATTCCTTACCCAATAGATGAAATAAACCTGGATTTTGAAGTGTTAGGCCATTCAGCCA
AGAGCTCATCACTGCTTGATGTTTTAATCGTGGCATCAAGGGCAGAAAACGTTAATACCCGTGTTGAAGCAGCCCATAGA
GCAGGGCTGGAACCCAAAGTTGTTGACGTTGAGTCTTATGCAGTAGAGCGTTCAGTACAACAAATCGCGCAAGAATTACC
AGCAAGTGGGCAAGACAAAATTGTTGCAATAGTAGATATTGGAGCCAATTATACTCACTTATTTGTTTTGCATGGTATGC
GACTAATCTTTTCAAGAGAAGAAAAATTTGGAGGTATGCAACTTGTTGAAGCTATTGCAGAACACTATCATATGAATTTG
GAGCAGGCTATTGCCCTAAAAAATCAGGGTAAACTACCGGAGGACTACGAATCTGCTGTATTAGAACCTTTTAAGGACAT
GATTCTTTTGCAGATCAAACGTACTTTGCAGTTTTTTTATTCAACCAGTCAACATGGATTTGTAGACCATATTTTATTGG
CGGGAGGAATGGCAAGGCAATCAGGATTGGCAGCGCTTATTCAAGAACAGTTAGGAGTGACTACTACAGTGGCAAATCCA
CTATCCCATATGTCTTTTGGTAAAAAAGTGAATTTGGATGTGATTAACAGTGATGCGCCTACACTGATGGTGGCTTGTGG
GTTGGCTTTGAGGCATGTCGAGTAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-352)

3D structure

• AlphaFold DB: Q5ZX06

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Legionella pneumophila strain ERS1305867	99.435	100	0.994
comM	Acinetobacter nosocomialis M2	42.817	100	0.429
pilM	Acinetobacter baumannii D1279779	42.535	100	0.427
comM	Acinetobacter baylyi ADP1	41.457	100	0.418