Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   ACI703_RS04225 Genome accession   NZ_CP173765
Coordinates   953076..954185 (+) Length   369 a.a.
NCBI ID   WP_234455948.1    Uniprot ID   -
Organism   Isoptericola jiangsuensis strain JSH01     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 948076..959185
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACI703_RS04210 (ACI703_04210) - 948718..949455 (+) 738 WP_406234875.1 hypothetical protein -
  ACI703_RS04215 (ACI703_04215) - 949516..950178 (-) 663 WP_406234877.1 hypothetical protein -
  ACI703_RS04220 (ACI703_04220) - 950487..952907 (-) 2421 WP_406234879.1 penicillin-binding protein 1A -
  ACI703_RS04225 (ACI703_04225) pilM 953076..954185 (+) 1110 WP_234455948.1 pilus assembly protein PilM Machinery gene
  ACI703_RS04230 (ACI703_04230) - 954185..955081 (+) 897 WP_406234881.1 PilN domain-containing protein -
  ACI703_RS04235 (ACI703_04235) - 955078..955731 (+) 654 WP_108769846.1 type 4a pilus biogenesis protein PilO -
  ACI703_RS04240 (ACI703_04240) - 955770..956264 (+) 495 WP_420883841.1 pilus assembly protein PilP -
  ACI703_RS04245 (ACI703_04245) - 956334..958256 (+) 1923 WP_420883842.1 type IV pilus secretin PilQ -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 39487.36 Da        Isoelectric Point: 4.5585

>NTDB_id=1073973 ACI703_RS04225 WP_234455948.1 953076..954185(+) (pilM) [Isoptericola jiangsuensis strain JSH01]
MRGRRSCVQVPVGRGETVGLIPKSQSPLIGVDISSTAVKLLQLSRSGNRFRVEHYAVEPLPPNAVVEKNIVEVEAVGEAI
RRAMNRSGSKAKLAAAAVAGSAVITKVIPMPADLDEGDMEAQIELEAVNYIPYPIEEVNLDFEVIGAIPNNPEMVQVLLA
ASRSENVELRQSALELGGLQAKVMDVEAFAVENAFALVASELPVSHDGVVALVDIGATMTTLNVLRGGRSLYSREQVFGG
KQLTDEIMRRYGLSYEEAGLAKRQGGLPESYEVEVLEPFKEATVQQISRLLQFFYAGSEFNRVDHIVLAGGCAALAGLPE
MVEEQLGVPTVVANPLAQMTLGPKVQAQALAQDAPALMIATGLALRSFD

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=1073973 ACI703_RS04225 WP_234455948.1 953076..954185(+) (pilM) [Isoptericola jiangsuensis strain JSH01]
ATGCGCGGACGCAGATCCTGTGTCCAAGTGCCCGTCGGCAGGGGAGAAACCGTGGGGCTCATCCCAAAAAGTCAGTCGCC
GCTCATTGGCGTCGACATCAGTTCGACTGCGGTAAAGCTGTTGCAGCTTTCCCGCAGCGGCAACCGTTTCCGCGTGGAAC
ACTACGCCGTGGAACCGTTGCCTCCGAATGCTGTGGTTGAAAAGAACATCGTCGAAGTGGAGGCCGTGGGTGAGGCCATC
CGCCGGGCGATGAACCGTTCCGGCAGCAAGGCCAAGCTGGCCGCGGCCGCCGTGGCCGGTTCGGCGGTGATCACCAAGGT
GATCCCGATGCCGGCCGACCTCGACGAAGGCGACATGGAAGCCCAGATCGAGCTGGAAGCGGTCAACTACATTCCGTACC
CGATCGAGGAAGTGAACCTGGACTTCGAGGTGATCGGGGCGATCCCGAACAACCCGGAGATGGTCCAGGTGCTGCTGGCT
GCCTCGCGCTCGGAAAACGTCGAGCTGCGCCAGTCGGCGCTGGAGCTGGGCGGCCTGCAGGCCAAGGTGATGGACGTGGA
GGCCTTCGCGGTCGAGAACGCCTTCGCCCTGGTGGCCAGCGAGCTGCCGGTGTCCCACGACGGCGTGGTGGCCCTGGTCG
ACATCGGCGCCACCATGACCACGCTGAACGTCCTGCGCGGCGGCCGCAGCCTGTACAGCCGCGAACAGGTGTTCGGCGGC
AAGCAGCTGACCGATGAGATCATGCGCCGCTACGGCCTGAGCTACGAGGAAGCCGGTCTGGCCAAGCGCCAGGGCGGGCT
GCCGGAAAGCTACGAAGTGGAAGTGCTGGAGCCCTTCAAGGAAGCGACGGTCCAGCAGATCAGCCGCCTGCTGCAGTTCT
TCTACGCGGGCAGTGAATTCAACCGGGTCGACCACATCGTGCTCGCCGGCGGTTGCGCCGCGCTGGCCGGTCTGCCGGAG
ATGGTCGAAGAGCAGCTGGGCGTGCCGACCGTGGTCGCCAACCCGCTGGCACAGATGACCCTGGGCCCGAAGGTGCAGGC
GCAGGCGCTTGCCCAGGATGCCCCCGCGCTGATGATCGCTACCGGTCTGGCACTGAGGAGCTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(30-368)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

52.286

94.851

0.496
  comM Acinetobacter nosocomialis M2

52.286

94.851

0.496
  comM Acinetobacter baylyi ADP1

50.571

94.851

0.48
  pilM Legionella pneumophila strain ERS1305867

47.578

95.122

0.453

Multiple sequence alignment