Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA/cflA   Type   Machinery gene
Locus tag   ACAM22_RS00165 Genome accession   NZ_AP028929
Coordinates   30815..32113 (+) Length   432 a.a.
NCBI ID   WP_261052231.1    Uniprot ID   -
Organism   Streptococcus sp. SN-1     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 25815..37113
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACAM22_RS00140 (MASAN616_00240) rpsB 26621..27400 (+) 780 WP_000268459.1 30S ribosomal protein S2 -
  ACAM22_RS00145 (MASAN616_00250) tsf 27479..28519 (+) 1041 WP_369606774.1 translation elongation factor Ts -
  ACAM22_RS00150 (MASAN616_00260) cysK 28641..29567 (-) 927 WP_261052232.1 cysteine synthase A -
  ACAM22_RS00155 (MASAN616_00270) - 29664..30107 (-) 444 WP_050223247.1 PH domain-containing protein -
  ACAM22_RS00160 (MASAN616_00280) - 30123..30758 (-) 636 WP_084926268.1 YigZ family protein -
  ACAM22_RS00165 (MASAN616_00290) comFA/cflA 30815..32113 (+) 1299 WP_261052231.1 DEAD/DEAH box helicase Machinery gene
  ACAM22_RS00170 (MASAN616_00300) comFC/cflB 32110..32772 (+) 663 WP_261052230.1 ComF family protein Machinery gene
  ACAM22_RS00175 (MASAN616_00310) raiA 32852..33400 (+) 549 WP_261052229.1 ribosome-associated translation inhibitor RaiA -
  ACAM22_RS00180 (MASAN616_00320) - 33536..35509 (+) 1974 WP_261052228.1 DHH family phosphoesterase -
  ACAM22_RS00185 (MASAN616_00330) rplI 35506..35958 (+) 453 WP_000864214.1 50S ribosomal protein L9 -

Sequence

Protein


Download         Length: 432 a.a.        Molecular weight: 49627.51 Da        Isoelectric Point: 8.4382

>NTDB_id=107298 ACAM22_RS00165 WP_261052231.1 30815..32113(+) (comFA/cflA) [Streptococcus sp. SN-1]
MKVNPNYLGRLFTENELTEEERQLAEKLPAMRKEKEKLFCQRCNSIILEEWYLPIGAYYCRECLLMKRVRSDQALYYFPQ
EDFPKQDVLKWRGQLTPFQEKVSEGLIRAVDKQEPTLVHAVTGAGKTEMIYQVVAKVINAGGAVCLASPRIDVCLELYKR
LQDDFACEIALLHGESEPYFRTPLVVATTHQLLKFYQAFDLLIVDEVDAFPYVDNPTLYHAVKNSVKENGLRIFLTATST
DELDRKVRIGELKRLSLPRRFHGNPLIIPKPVWLSDFNRYLDKNRLSPKLKSYIEKQRKTSYPLLIFASEIKKGGQLKEI
LQEQFPNEKIGFVSSVTEDRLEKVQAFRDGELTILISTTILERGVTFPCVDVFVVEANHRLFTKSSLIQIGGRVGRSMDR
PTGDLLFFHDGLNASIKKAIKEIQQMNKEAGL

Nucleotide


Download         Length: 1299 bp        

>NTDB_id=107298 ACAM22_RS00165 WP_261052231.1 30815..32113(+) (comFA/cflA) [Streptococcus sp. SN-1]
ATGAAAGTAAATCCAAACTATCTCGGTCGTTTGTTTACTGAGAATGAATTAACAGAAGAGGAGCGTCAGTTGGCGGAGAA
ACTTCCAGCAATGAGAAAGGAGAAGGAGAAACTTTTCTGTCAACGTTGTAATAGTATTATTCTAGAGGAATGGTATTTGC
CCATCGGTGCTTACTATTGTCGAGAGTGCTTGCTGATGAAGCGAGTCAGGAGTGACCAAGCTTTATACTATTTTCCGCAG
GAGGATTTTCCTAAACAGGATGTTCTTAAATGGCGTGGTCAATTAACTCCTTTTCAAGAGAAGGTGTCAGAGGGACTAAT
TCGAGCTGTCGACAAGCAAGAGCCAACCTTGGTACATGCGGTGACAGGAGCTGGAAAGACGGAAATGATTTATCAAGTAG
TGGCTAAAGTGATTAATGCGGGAGGTGCAGTGTGTTTGGCCAGTCCTCGCATAGATGTTTGTTTAGAGCTGTATAAGCGC
CTGCAAGATGATTTTGCTTGCGAGATAGCCCTCCTACATGGAGAATCGGAACCTTATTTTCGAACACCACTAGTTGTTGC
GACGACCCATCAGTTATTGAAGTTTTATCAAGCTTTTGATTTGCTGATAGTGGATGAAGTAGATGCTTTTCCTTATGTTG
ATAATCCCACGCTTTACCATGCTGTCAAGAATAGTGTAAAGGAGAATGGGCTGAGAATCTTTTTAACAGCGACTTCGACC
GATGAGTTAGATAGGAAGGTTCGCATAGGAGAATTAAAACGATTGAGTTTGCCAAGACGATTTCATGGGAATCCGTTGAT
TATCCCTAAACCAGTTTGGTTATCTGATTTTAATCGCTACTTAGATAAGAATCGTTTGTCACCAAAGTTAAAGTCCTATA
TTGAGAAGCAGAGAAAGACATCTTATCCGTTACTCATTTTTGCATCAGAAATTAAGAAAGGGGGACAGTTAAAAGAAATC
TTACAGGAGCAATTTCCAAATGAGAAAATTGGTTTTGTATCGTCTGTAACAGAAGATCGATTAGAGAAAGTACAAGCTTT
TCGAGATGGAGAATTGACAATACTTATCAGTACTACAATCTTGGAGCGTGGAGTTACTTTCCCTTGTGTGGATGTTTTCG
TAGTGGAGGCCAATCATCGTCTGTTTACCAAGTCTAGTTTGATTCAGATTGGTGGACGAGTTGGGCGAAGCATGGATAGA
CCGACAGGAGATTTACTTTTCTTCCATGATGGATTAAATGCTTCAATCAAGAAGGCAATTAAGGAAATTCAGCAGATGAA
CAAGGAGGCAGGCTTATGA

Domains

Predicted by InterproScan.

Type III restriction enzyme, res subunit (ResIII)

(94-238)

Helicase conserved C-terminal domain (Helicase_C)

(293-396)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA/cflA Streptococcus pneumoniae Rx1

94.907

100

0.949
  comFA/cflA Streptococcus pneumoniae D39

94.907

100

0.949
  comFA/cflA Streptococcus pneumoniae R6

94.907

100

0.949
  comFA/cflA Streptococcus pneumoniae TIGR4

94.676

100

0.947
  comFA/cflA Streptococcus mitis NCTC 12261

94.676

100

0.947
  comFA/cflA Streptococcus mitis SK321

94.444

100

0.944
  comFA Lactococcus lactis subsp. cremoris KW2

50.35

99.306

0.5
  comFA Latilactobacillus sakei subsp. sakei 23K

39.211

99.769

0.391
  comFA Bacillus subtilis subsp. subtilis str. 168

39.312

94.213

0.37

Multiple sequence alignment