Detailed information NTG1072181

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: ACI3KK_RS11590
• Genome accession: NZ_CP173421
• Coordinates: 2314556..2315788 (+)
• Length: 410 a.a.
• NCBI ID: WP_405121008.1
• Uniprot ID: -
• Organism: Pseudomonas leptonychotis strain TMP18
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2309556..2320788

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACI3KK_RS11570 (ACI3KK_11570)	-	2310036..2311622 (-)	1587	WP_136664701.1	ABC-F family ATPase	-
ACI3KK_RS11575 (ACI3KK_11575)	sph	2312109..2313623 (-)	1515	WP_405121007.1	sphingomyelin phosphodiesterase	-
ACI3KK_RS11580 (ACI3KK_11580)	-	2313757..2314125 (-)	369	WP_405121128.1	MmcQ/YjbR family DNA-binding protein	-
ACI3KK_RS11585 (ACI3KK_11585)	-	2314134..2314412 (-)	279	WP_090253882.1	peptidylprolyl isomerase	-
ACI3KK_RS11590 (ACI3KK_11590)	pilU	2314556..2315788 (+)	1233	WP_405121008.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
ACI3KK_RS11595 (ACI3KK_11595)	-	2315795..2317750 (-)	1956	WP_405121009.1	acetoacetate--CoA ligase	-
ACI3KK_RS11600 (ACI3KK_11600)	-	2317978..2318880 (-)	903	WP_405121010.1	hydroxymethylglutaryl-CoA lyase	-

Sequence

Protein

• Length: 410 a.a.
• Molecular weight: 44906.24 Da
• Isoelectric Point: 5.5504

>NTDB_id=1072181 ACI3KK_RS11590 WP_405121008.1 2314556..2315788(+) (pilU) [Pseudomonas leptonychotis strain TMP18]
MDLNAMLKILSSQDGSDLYLSTGAPPCAKFNGVLKALSAEPLKAGEVADIADAVMDVAQREEFERELEMNLAISIAGVGR
FRINIFKQRNEVSIVARNIKLDIPKFEDLKLPQVLLKTVMEKRGLVLFVGGTGSGKSTSLAALIDYRNRNSGGHIITIED
PVEYVHKHKKSIINQREVGVDTRSFHAALKNTLRQAPDVILIGEIRDRETMEHALAFADTGHLAISTLHANNANQALDRI
INFFPEDRRPQLLNDLGNNLKAFVSQRLVKTIDGKRRAAVEVLLGTPTIRDLIKRNEFSEIKEIMEKSKNLGMQTFDQAL
IDLVNDGSIDEDEAVKNADSANNVRLKLKLYRDNPAAPVQAAAASAPAPAAAEARSTTPAASAGEWGLELTLEDIEEEPL
PPEDPGRKGI

Nucleotide

• Length: 1233 bp

>NTDB_id=1072181 ACI3KK_RS11590 WP_405121008.1 2314556..2315788(+) (pilU) [Pseudomonas leptonychotis strain TMP18]
ATGGATCTCAACGCCATGCTGAAAATCCTGTCCAGCCAGGACGGGTCTGATCTTTACCTTTCGACTGGAGCACCGCCGTG
CGCCAAGTTCAATGGCGTGCTCAAGGCGTTGAGTGCTGAACCGCTGAAAGCCGGCGAGGTGGCGGATATTGCAGACGCGG
TGATGGATGTGGCGCAGCGTGAAGAATTCGAGCGCGAGTTAGAGATGAACCTGGCTATTTCCATTGCTGGCGTCGGGCGC
TTTCGTATCAATATCTTCAAGCAGCGCAACGAAGTGTCGATTGTGGCGCGCAATATCAAGCTGGACATCCCCAAGTTTGA
AGACCTTAAACTTCCGCAGGTGCTGCTCAAGACGGTGATGGAAAAGCGCGGCCTGGTGCTGTTTGTTGGCGGCACGGGCT
CGGGTAAATCGACCTCGCTGGCCGCGTTGATTGATTACCGTAACCGCAACAGCGGTGGCCACATCATCACCATCGAAGAC
CCGGTGGAGTATGTACACAAGCACAAAAAGTCGATCATCAACCAACGCGAAGTCGGCGTAGATACCCGCAGTTTTCACGC
GGCACTGAAGAACACCCTGCGCCAGGCCCCAGACGTGATCCTGATCGGCGAAATTCGCGACCGCGAAACCATGGAACATG
CCCTGGCCTTTGCCGACACCGGCCACCTGGCAATTTCCACCCTACACGCCAACAACGCCAACCAGGCGCTGGACCGCATC
ATCAACTTCTTCCCGGAAGACCGCCGCCCGCAACTGCTTAACGACCTGGGTAACAACCTCAAAGCATTCGTCTCGCAGCG
CCTGGTGAAAACCATCGACGGTAAACGCCGCGCGGCGGTTGAAGTACTGCTGGGCACGCCGACCATTCGCGACCTGATCA
AACGCAACGAGTTTTCCGAGATCAAGGAGATCATGGAGAAATCGAAGAACCTCGGCATGCAGACATTCGACCAGGCACTG
ATTGACCTGGTCAACGATGGCTCGATTGACGAAGACGAAGCGGTGAAAAATGCCGACTCGGCAAACAACGTGCGATTGAA
GCTCAAACTGTATCGCGACAATCCAGCTGCGCCGGTGCAGGCCGCTGCAGCCTCGGCACCCGCTCCAGCGGCTGCCGAGG
CACGGTCGACAACCCCAGCCGCTTCAGCGGGCGAGTGGGGGCTGGAGCTGACGCTGGAGGATATCGAAGAGGAGCCACTG
CCGCCTGAAGACCCAGGGCGCAAAGGCATCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-248)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	56.41	85.61	0.483
pilU	Acinetobacter baylyi ADP1	52.557	85.854	0.451
pilU	Vibrio cholerae strain A1552	52.125	86.098	0.449
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	38.734	96.341	0.373