Detailed information NTG1072114

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: ACI2O8_RS13275
• Genome accession: NZ_CP173415
• Coordinates: 2552702..2553319 (-)
• Length: 205 a.a.
• NCBI ID: WP_004398514.1
• Uniprot ID: A0AAE2SLR2
• Organism: Bacillus subtilis strain FJYA24
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2547702..2558319

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACI2O8_RS13245 (ACI2O8_13245)	rpsT	2548011..2548277 (+)	267	WP_003229989.1	30S ribosomal protein S20	-
ACI2O8_RS13250 (ACI2O8_13250)	holA	2548292..2549335 (-)	1044	WP_015714286.1	DNA polymerase III subunit delta	-
ACI2O8_RS13255 (ACI2O8_13255)	-	2549375..2549524 (-)	150	WP_003229985.1	hypothetical protein	-
ACI2O8_RS13260 (ACI2O8_13260)	yqzM	2549565..2549699 (+)	135	WP_003229983.1	YqzM family protein	-
ACI2O8_RS13265 (ACI2O8_13265)	comEC	2549739..2552069 (-)	2331	WP_074794436.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
ACI2O8_RS13270 (ACI2O8_13270)	comEB	2552066..2552635 (-)	570	WP_015714288.1	ComE operon protein 2	-
ACI2O8_RS13275 (ACI2O8_13275)	comEA	2552702..2553319 (-)	618	WP_004398514.1	competence protein ComEA	Machinery gene
ACI2O8_RS13280 (ACI2O8_13280)	comER	2553403..2554224 (+)	822	WP_004398597.1	late competence protein ComER	-
ACI2O8_RS13285 (ACI2O8_13285)	yqeM	2554290..2555033 (-)	744	WP_003229973.1	class I SAM-dependent methyltransferase	-
ACI2O8_RS13290 (ACI2O8_13290)	rsfS	2555030..2555386 (-)	357	WP_003229971.1	ribosome silencing factor	-
ACI2O8_RS13295 (ACI2O8_13295)	yqeK	2555404..2555964 (-)	561	WP_004399059.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
ACI2O8_RS13300 (ACI2O8_13300)	nadD	2555954..2556523 (-)	570	WP_004398676.1	nicotinate-nucleotide adenylyltransferase	-
ACI2O8_RS13305 (ACI2O8_13305)	yhbY	2556535..2556825 (-)	291	WP_003226133.1	ribosome assembly RNA-binding protein YhbY	-
ACI2O8_RS13310 (ACI2O8_13310)	aroE	2556819..2557661 (-)	843	WP_404977969.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 205 a.a.
• Molecular weight: 21769.45 Da
• Isoelectric Point: 4.7220

>NTDB_id=1072114 ACI2O8_RS13275 WP_004398514.1 2552702..2553319(-) (comEA) [Bacillus subtilis strain FJYA24]
MNWLNQHKKAIILAASAAVFTAIMIFLATGKNKEPVKQAVPTETENTVVKQEANNDESNETIVIDIKGAVQHPGVYEMRT
GDRVSQAIEKAGGTSEQADEAQVNLAEILQDGTVVYIPKKGEETAVQQGGGGSVQSDGGKGALVNINTATLEELQGISGV
GPSKAEAIIAYREENGRFQTIEDITKVSGIGEKSFEKIKSSITVK

Nucleotide

• Length: 618 bp

>NTDB_id=1072114 ACI2O8_RS13275 WP_004398514.1 2552702..2553319(-) (comEA) [Bacillus subtilis strain FJYA24]
ATGAATTGGTTGAATCAGCATAAGAAAGCAATTATTTTAGCGGCTTCTGCGGCTGTTTTCACAGCGATTATGATCTTTCT
GGCCACAGGGAAAAATAAAGAGCCGGTGAAGCAAGCTGTACCAACAGAGACAGAAAATACAGTGGTAAAGCAGGAAGCAA
ACAACGACGAGTCAAACGAAACAATTGTGATAGACATCAAAGGTGCTGTTCAGCATCCTGGCGTTTATGAAATGCGAACA
GGGGACAGAGTATCTCAGGCAATTGAGAAAGCGGGCGGGACCAGTGAACAAGCAGACGAAGCGCAAGTAAATTTGGCGGA
GATTCTGCAGGACGGGACAGTGGTGTACATCCCGAAAAAGGGAGAGGAAACAGCAGTGCAGCAAGGTGGCGGAGGGTCTG
TCCAAAGCGATGGAGGGAAGGGAGCGCTGGTGAATATCAATACAGCAACCTTAGAGGAGTTACAAGGCATCTCAGGGGTG
GGGCCATCCAAAGCTGAAGCTATTATTGCATACCGGGAAGAAAACGGTCGTTTCCAAACAATTGAAGATATCACTAAGGT
TTCAGGAATAGGTGAAAAGTCATTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (142-203)

• SLBB domain (SLBB) (63-118)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	100	100	1
comEA	Staphylococcus aureus MW2	37.273	100	0.4
comEA	Staphylococcus aureus N315	36.364	100	0.39