Detailed information NTG1071612

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: ACI1PX_RS02000
• Genome accession: NZ_CP173359
• Coordinates: 431089..432237 (+)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa isolate CF2843GFP
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 426089..437237

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACI1PX_RS01975 (ACI1PX_01975)	-	427240..427536 (-)	297	WP_031628073.1	DUF167 domain-containing protein	-
ACI1PX_RS01980 (ACI1PX_01980)	-	427533..428126 (-)	594	WP_003084544.1	YggT family protein	-
ACI1PX_RS01985 (ACI1PX_01985)	proC	428137..428958 (-)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
ACI1PX_RS01990 (ACI1PX_01990)	-	428970..429662 (-)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
ACI1PX_RS01995 (ACI1PX_01995)	pilT	429877..430911 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
ACI1PX_RS02000 (ACI1PX_02000)	pilU	431089..432237 (+)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
ACI1PX_RS02005 (ACI1PX_02005)	czcD	432245..433144 (-)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
ACI1PX_RS02010 (ACI1PX_02010)	-	433298..433702 (+)	405	WP_003084560.1	NINE protein	-
ACI1PX_RS02015 (ACI1PX_02015)	-	433945..435318 (+)	1374	WP_003160915.1	pyridoxal-phosphate dependent enzyme	-
ACI1PX_RS02020 (ACI1PX_02020)	-	435315..436499 (+)	1185	WP_014602386.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=1071612 ACI1PX_RS02000 WP_003100959.1 431089..432237(+) (pilU) [Pseudomonas aeruginosa isolate CF2843GFP]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=1071612 ACI1PX_RS02000 WP_003100959.1 431089..432237(+) (pilU) [Pseudomonas aeruginosa isolate CF2843GFP]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382