Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   ACIX90_RS04990 Genome accession   NZ_CP173319
Coordinates   1078273..1078719 (-) Length   148 a.a.
NCBI ID   WP_215577633.1    Uniprot ID   -
Organism   Actinobacillus pleuropneumoniae strain APL2401251E     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1073273..1083719
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACIX90_RS04965 (ACIX90_04965) yacG 1074180..1074368 (-) 189 WP_404811257.1 DNA gyrase inhibitor YacG -
  ACIX90_RS04970 (ACIX90_04970) coaE 1074350..1074988 (-) 639 WP_005601202.1 dephospho-CoA kinase -
  ACIX90_RS04975 (ACIX90_04975) - 1075010..1075648 (-) 639 WP_005604550.1 prepilin peptidase -
  ACIX90_RS04980 (ACIX90_04980) pilC 1075675..1076877 (-) 1203 WP_404811258.1 type II secretion system F family protein Machinery gene
  ACIX90_RS04985 (ACIX90_04985) pilB 1076861..1078255 (-) 1395 WP_005601209.1 GspE/PulE family protein Machinery gene
  ACIX90_RS04990 (ACIX90_04990) pilA 1078273..1078719 (-) 447 WP_215577633.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  ACIX90_RS04995 (ACIX90_04995) radA 1078901..1080280 (+) 1380 WP_005601211.1 DNA repair protein RadA Machinery gene
  ACIX90_RS05000 (ACIX90_05000) - 1080324..1080557 (-) 234 WP_005597399.1 YgjV family protein -
  ACIX90_RS05005 (ACIX90_05005) folA 1080684..1081172 (-) 489 WP_005597400.1 type 3 dihydrofolate reductase -
  ACIX90_RS05010 (ACIX90_05010) proB 1081259..1082350 (+) 1092 WP_005601213.1 glutamate 5-kinase -
  ACIX90_RS05015 (ACIX90_05015) - 1082363..1083139 (+) 777 WP_005597402.1 TSUP family transporter -

Sequence

Protein


Download         Length: 148 a.a.        Molecular weight: 15962.35 Da        Isoelectric Point: 7.6257

>NTDB_id=1071544 ACIX90_RS04990 WP_215577633.1 1078273..1078719(-) (pilA) [Actinobacillus pleuropneumoniae strain APL2401251E]
MQKLSLIRPLTNAFTLIELMIVIAIIAILATVAIPSYNSYTQKAALSELLAASASYKTDVEICIYNTGDSKNCSGGQNGV
RKITELRQAKYLNAITVEGGTITVTGKGNLQEYGYTMTPIHNGSTISWETKCKGEDLSLFPANFCASN

Nucleotide


Download         Length: 447 bp        

>NTDB_id=1071544 ACIX90_RS04990 WP_215577633.1 1078273..1078719(-) (pilA) [Actinobacillus pleuropneumoniae strain APL2401251E]
ATGCAAAAACTAAGTCTTATTCGACCGCTTACTAACGCGTTTACTTTAATTGAATTGATGATCGTGATTGCGATTATTGC
CATTTTAGCTACGGTTGCAATTCCGTCATATAACAGTTATACCCAAAAAGCGGCGCTTTCGGAGCTATTGGCGGCATCGG
CTTCTTATAAAACGGATGTCGAGATCTGCATATATAACACCGGAGATTCTAAAAACTGTAGCGGCGGTCAAAACGGTGTC
AGAAAAATAACGGAGCTTAGACAGGCTAAATATTTAAATGCCATTACGGTGGAAGGCGGAACGATTACGGTAACGGGGAA
GGGGAATCTGCAGGAATACGGTTATACGATGACACCGATTCATAACGGTAGCACTATTTCTTGGGAAACGAAATGTAAAG
GGGAGGACTTAAGTTTATTTCCGGCAAATTTCTGCGCATCAAATTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(13-33)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Glaesserella parasuis strain SC1401

68.919

100

0.689
  pilA Haemophilus influenzae 86-028NP

55.102

99.324

0.547
  pilA Haemophilus influenzae Rd KW20

53.061

99.324

0.527

Multiple sequence alignment