Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   ACIX90_RS04980 Genome accession   NZ_CP173319
Coordinates   1075675..1076877 (-) Length   400 a.a.
NCBI ID   WP_404811258.1    Uniprot ID   -
Organism   Actinobacillus pleuropneumoniae strain APL2401251E     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1070675..1081877
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACIX90_RS04955 (ACIX90_04955) lptE 1072475..1072969 (+) 495 WP_005597385.1 LPS assembly lipoprotein LptE -
  ACIX90_RS04960 (ACIX90_04960) holA 1073078..1074103 (+) 1026 WP_012263005.1 DNA polymerase III subunit delta -
  ACIX90_RS04965 (ACIX90_04965) yacG 1074180..1074368 (-) 189 WP_404811257.1 DNA gyrase inhibitor YacG -
  ACIX90_RS04970 (ACIX90_04970) coaE 1074350..1074988 (-) 639 WP_005601202.1 dephospho-CoA kinase -
  ACIX90_RS04975 (ACIX90_04975) - 1075010..1075648 (-) 639 WP_005604550.1 prepilin peptidase -
  ACIX90_RS04980 (ACIX90_04980) pilC 1075675..1076877 (-) 1203 WP_404811258.1 type II secretion system F family protein Machinery gene
  ACIX90_RS04985 (ACIX90_04985) pilB 1076861..1078255 (-) 1395 WP_005601209.1 GspE/PulE family protein Machinery gene
  ACIX90_RS04990 (ACIX90_04990) pilA 1078273..1078719 (-) 447 WP_215577633.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  ACIX90_RS04995 (ACIX90_04995) radA 1078901..1080280 (+) 1380 WP_005601211.1 DNA repair protein RadA Machinery gene
  ACIX90_RS05000 (ACIX90_05000) - 1080324..1080557 (-) 234 WP_005597399.1 YgjV family protein -
  ACIX90_RS05005 (ACIX90_05005) folA 1080684..1081172 (-) 489 WP_005597400.1 type 3 dihydrofolate reductase -

Sequence

Protein


Download         Length: 400 a.a.        Molecular weight: 45648.14 Da        Isoelectric Point: 10.3239

>NTDB_id=1071542 ACIX90_RS04980 WP_404811258.1 1075675..1076877(-) (pilC) [Actinobacillus pleuropneumoniae strain APL2401251E]
MLKVYEFHWKAKNRFQQLQKGKALACHRTELERRLLAKGYSQIRIQRNFRFVTQASSESITQVIKQLALLLNAAVPLKQA
LNMLLENTYHIRIYMWLNDLNRLIESGYTMSSSLTKLNLYLAKSEIQLVRIGEQSGRLGEILQNIAVNRHQTEKLHKKIK
KIMFYPVVTLIISVALSLAMLLFIVPQFIELYSGREKPLPLITQMLFTLSHFLQHSLLQVTIVSLLAVGFFRFFAKKYRF
LTAIKNGMLSALPIFNKIVENVRIVFFSQNLGLMLKTHIRLETALNTFLATQSQDLRLQKEIENILAQLKQGYRFSESIN
ASVFGTQVVQMLAIGEQSGNVAQMCSYIGELYRQQLDDQIELLSQLLEPILMLIIGLIIGTVLIGLYLPIFDLGGLVGGG

Nucleotide


Download         Length: 1203 bp        

>NTDB_id=1071542 ACIX90_RS04980 WP_404811258.1 1075675..1076877(-) (pilC) [Actinobacillus pleuropneumoniae strain APL2401251E]
ATGTTGAAAGTGTATGAATTTCATTGGAAAGCTAAAAATCGTTTTCAACAACTTCAGAAAGGAAAAGCGTTAGCTTGTCA
TCGAACGGAATTGGAACGGCGTTTATTAGCAAAAGGCTATTCACAGATCCGTATCCAACGTAATTTTCGTTTTGTCACAC
AGGCTTCTTCAGAATCGATTACCCAAGTAATTAAACAGTTGGCGCTTTTACTGAATGCCGCAGTCCCTTTAAAACAAGCG
CTGAATATGTTGTTAGAAAATACTTATCATATTCGGATCTATATGTGGCTGAATGATTTAAACCGTTTGATAGAATCGGG
TTATACAATGTCGTCAAGTTTAACCAAATTGAATTTATATTTGGCGAAATCGGAAATACAGCTGGTACGGATTGGCGAAC
AAAGCGGGCGATTAGGCGAAATTTTGCAAAATATTGCGGTAAATCGCCATCAAACCGAAAAATTACATAAGAAAATAAAA
AAGATAATGTTTTATCCTGTCGTCACTTTGATTATTTCCGTTGCGCTTTCTCTCGCCATGCTCCTTTTTATCGTGCCGCA
ATTTATCGAGCTGTATTCCGGTCGTGAAAAGCCGCTTCCTTTAATTACACAAATGCTATTTACATTATCGCACTTCTTAC
AACATTCTTTATTACAGGTAACGATAGTCAGTTTATTAGCGGTCGGTTTTTTTCGTTTTTTTGCAAAAAAATATCGATTT
TTAACCGCTATAAAAAACGGGATGTTAAGTGCTTTGCCTATTTTTAATAAGATAGTCGAAAACGTTCGTATAGTATTTTT
TAGTCAAAATCTCGGCTTGATGCTAAAAACTCATATTCGGCTGGAAACGGCGTTAAATACCTTTCTTGCTACACAATCGC
AAGATTTGAGGTTACAAAAAGAGATAGAAAATATTTTAGCGCAATTAAAACAAGGTTATCGCTTTTCCGAAAGTATTAAT
GCGAGTGTGTTCGGTACTCAAGTAGTACAAATGCTTGCCATTGGCGAACAGAGTGGGAATGTAGCGCAGATGTGTAGCTA
TATCGGGGAGTTATATCGACAACAATTGGATGATCAGATTGAACTGTTATCGCAGCTGCTTGAGCCGATCTTAATGCTGA
TTATCGGATTAATTATCGGTACGGTTTTGATCGGATTATATTTACCGATTTTTGATTTAGGCGGATTAGTCGGAGGCGGT
TAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(267-389)

Type II secretion system (T2SS), protein F (T2SSF)

(64-186)

HEPN-like integron domain (HEPN-like_int)

(160-280)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Glaesserella parasuis strain SC1401

50.126

99.25

0.498
  pilC Haemophilus influenzae Rd KW20

39.75

100

0.398
  pilC Haemophilus influenzae 86-028NP

38.75

100

0.388

Multiple sequence alignment