Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   ACIX3D_RS04655 Genome accession   NZ_CP173318
Coordinates   1029185..1030387 (-) Length   400 a.a.
NCBI ID   WP_005597394.1    Uniprot ID   Q6XFB0
Organism   Actinobacillus pleuropneumoniae strain APL2210214E     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1024185..1035387
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACIX3D_RS04630 (ACIX3D_04635) lptE 1025985..1026479 (+) 495 WP_005597385.1 LPS assembly lipoprotein LptE -
  ACIX3D_RS04635 (ACIX3D_04640) holA 1026588..1027613 (+) 1026 WP_005612206.1 DNA polymerase III subunit delta -
  ACIX3D_RS04640 (ACIX3D_04645) yacG 1027690..1027878 (-) 189 WP_005597388.1 DNA gyrase inhibitor YacG -
  ACIX3D_RS04645 (ACIX3D_04650) coaE 1027860..1028498 (-) 639 WP_005597390.1 dephospho-CoA kinase -
  ACIX3D_RS04650 (ACIX3D_04655) - 1028520..1029158 (-) 639 WP_005597392.1 prepilin peptidase -
  ACIX3D_RS04655 (ACIX3D_04660) pilC 1029185..1030387 (-) 1203 WP_005597394.1 type II secretion system F family protein Machinery gene
  ACIX3D_RS04660 (ACIX3D_04665) pilB 1030371..1031765 (-) 1395 WP_117374128.1 GspE/PulE family protein Machinery gene
  ACIX3D_RS04665 (ACIX3D_04670) pilA 1031783..1032229 (-) 447 WP_005597397.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  ACIX3D_RS04670 (ACIX3D_04675) radA 1032411..1033790 (+) 1380 WP_005597398.1 DNA repair protein RadA -
  ACIX3D_RS04675 (ACIX3D_04680) - 1033834..1034067 (-) 234 WP_005597399.1 YgjV family protein -
  ACIX3D_RS04680 (ACIX3D_04685) folA 1034194..1034682 (-) 489 WP_005597400.1 type 3 dihydrofolate reductase -

Sequence

Protein


Download         Length: 400 a.a.        Molecular weight: 45588.11 Da        Isoelectric Point: 10.2885

>NTDB_id=1071515 ACIX3D_RS04655 WP_005597394.1 1029185..1030387(-) (pilC) [Actinobacillus pleuropneumoniae strain APL2210214E]
MLKVYEFHWKAKNRFQQLQKGKALACHRTELERRLLAKGYSQIRIQRNFRFVTQASSESITQVIKQLALLLNAAVPLKQA
LNMLLENTYHIRIYMWLNDLNRLIESGYTMSSSLTKLNLYLAKSEIQLVRIGEQSGRLGEILQNIAVNRYQTEKLHKKIK
KIMFYPVVTLIISVALSLAMLLFIVPQFIELYSGREKPLPLITQMLFTLSLFLQHSLLQVTIVSLLAVGFFRFFAKKYRF
LTAIKNGVLSALPIFNKIVENVRIVFFSQNLGLMLKAHIRLETALNTFLATQSQDLRLQKEIENILAQLKQGYRFSESIN
ASVFGTQVVQMLAIGEQSGNVAQMCSYIGELYRQQLDDQIELLSQLLEPILMLIIGLIIGTVLIGLYLPIFDLGGLVGGG

Nucleotide


Download         Length: 1203 bp        

>NTDB_id=1071515 ACIX3D_RS04655 WP_005597394.1 1029185..1030387(-) (pilC) [Actinobacillus pleuropneumoniae strain APL2210214E]
ATGTTGAAAGTGTATGAATTCCATTGGAAAGCTAAAAATCGTTTTCAACAACTTCAGAAAGGAAAAGCGTTAGCTTGTCA
TCGAACGGAATTGGAACGGCGTTTATTAGCAAAAGGCTATTCACAGATCCGTATCCAACGTAATTTTCGTTTTGTCACAC
AGGCTTCTTCAGAATCGATTACTCAAGTAATTAAACAGTTGGCGCTTTTACTGAATGCCGCAGTCCCTTTAAAACAAGCG
CTGAATATGTTGTTAGAAAATACTTATCATATTCGGATCTATATGTGGCTGAATGATTTAAACCGTTTGATAGAATCGGG
TTATACAATGTCGTCAAGTTTAACCAAATTGAATTTATATTTGGCGAAATCGGAAATACAGCTGGTACGGATTGGCGAAC
AAAGCGGGCGATTAGGCGAAATTTTGCAAAATATTGCGGTAAATCGCTATCAAACCGAAAAATTACATAAGAAAATAAAA
AAGATAATGTTTTATCCTGTCGTCACTTTGATTATTTCCGTTGCGCTTTCTCTCGCCATGCTCCTTTTTATCGTGCCGCA
ATTTATCGAGCTGTATTCCGGTCGTGAAAAGCCGCTTCCTTTAATTACACAAATGCTATTTACATTATCGCTCTTCTTAC
AACATTCTTTATTACAGGTAACGATAGTCAGTTTATTAGCGGTCGGTTTTTTCCGTTTTTTTGCAAAAAAATATCGATTT
TTAACCGCTATAAAAAACGGGGTGTTAAGTGCTTTGCCTATTTTTAATAAGATAGTCGAAAACGTTCGTATAGTATTTTT
TAGTCAAAATCTCGGCTTGATGCTAAAAGCTCATATTCGGCTGGAAACGGCGTTAAATACCTTTCTTGCTACACAATCGC
AAGATTTGAGGTTACAAAAAGAGATAGAAAATATTTTAGCGCAATTAAAACAAGGTTATCGCTTTTCCGAAAGTATTAAT
GCGAGTGTGTTCGGTACTCAAGTAGTACAAATGCTTGCCATTGGCGAACAGAGTGGGAATGTAGCGCAGATGTGTAGCTA
TATCGGGGAGTTATATCGACAACAATTGGATGATCAGATTGAACTGTTATCGCAGCTGCTTGAGCCGATCTTAATGCTGA
TTATCGGATTAATTATCGGTACGGTTTTGATCGGATTATATTTACCGATTTTTGATTTAGGCGGACTAGTCGGAGGCGGT
TAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(267-389)

Type II secretion system (T2SS), protein F (T2SSF)

(64-186)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q6XFB0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Glaesserella parasuis strain SC1401

49.874

99.25

0.495
  pilC Haemophilus influenzae Rd KW20

40

100

0.4
  pilC Haemophilus influenzae 86-028NP

39

100

0.39

Multiple sequence alignment