Detailed information    

insolico Bioinformatically predicted

Overview

Name   comE   Type   Machinery gene
Locus tag   ACIO03_RS04220 Genome accession   NZ_CP173231
Coordinates   957892..959214 (-) Length   440 a.a.
NCBI ID   WP_264255778.1    Uniprot ID   -
Organism   Avibacterium paragallinarum strain npAP/OH-USA/20230213/S1-4     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 952892..964214
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACIO03_RS04195 (ACIO03_04195) secM 954435..954746 (-) 312 WP_264255773.1 secA translation cis-regulator SecM -
  ACIO03_RS04200 (ACIO03_04200) - 954853..955158 (+) 306 WP_035688115.1 DciA family protein -
  ACIO03_RS04205 (ACIO03_04205) - 955130..956020 (-) 891 WP_017806825.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -
  ACIO03_RS04210 (ACIO03_04210) aroB 956026..957114 (-) 1089 WP_264255775.1 3-dehydroquinate synthase -
  ACIO03_RS04215 (ACIO03_04215) aroK 957131..957691 (-) 561 WP_319019334.1 shikimate kinase AroK -
  ACIO03_RS04220 (ACIO03_04220) comE 957892..959214 (-) 1323 WP_264255778.1 type IV pilus secretin PilQ Machinery gene
  ACIO03_RS04225 (ACIO03_04225) - 959223..959582 (-) 360 WP_017806828.1 hypothetical protein -
  ACIO03_RS04230 (ACIO03_04230) - 959582..960130 (-) 549 WP_264255779.1 hypothetical protein -
  ACIO03_RS04235 (ACIO03_04235) - 960096..960608 (-) 513 WP_264255780.1 PilN domain-containing protein -
  ACIO03_RS04240 (ACIO03_04240) - 960638..961405 (-) 768 WP_035688099.1 hypothetical protein -
  ACIO03_RS04245 (ACIO03_04245) - 961564..964113 (+) 2550 WP_264255781.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 440 a.a.        Molecular weight: 48622.65 Da        Isoelectric Point: 6.1197

>NTDB_id=1070982 ACIO03_RS04220 WP_264255778.1 957892..959214(-) (comE) [Avibacterium paragallinarum strain npAP/OH-USA/20230213/S1-4]
MKLPNIIKNILLFFLILSYPFRQVNASDENNTFSVHLKQAPLVDTVQYLAGYQKINLVIDDELSGSISLQLQNTTLLQLL
KSIAEIKHLRFWENNGIYYLSQSQATNQENVVLNSSTDIATSADMLITSIKLHYAKASEVMKALTSGNGSLLTANGSISF
DERNNLLIIQDEAKKIKQIRKLVADMDKPIQQIIIEARIVTISDESLKELGVRWGIFSPSDISHSVSGSLNANGFSNITD
NLNINFATSSNPAGSVALQVAKINGRLLDLELTALERENNVEIIASPRLLTTNKKSASIKQGTEIPYVVNNGKNDMQSIE
FREAVLGLEVTPHLLKHNGILLDLVVSQNAPGGRVSYGENEVISIDKQEINTQVFAQDGETIVLGGIFHDTITKALDKVP
ILGDIPGIKRLFSKEVERHQKRELVIFVTPRLIRSGTVEE

Nucleotide


Download         Length: 1323 bp        

>NTDB_id=1070982 ACIO03_RS04220 WP_264255778.1 957892..959214(-) (comE) [Avibacterium paragallinarum strain npAP/OH-USA/20230213/S1-4]
ATGAAGTTACCAAATATAATAAAGAATATTCTTTTATTTTTTCTTATTCTATCCTATCCATTTAGACAGGTTAATGCAAG
TGACGAAAATAATACATTCTCTGTTCATTTAAAGCAAGCTCCATTAGTAGATACTGTACAATATTTAGCTGGGTATCAAA
AAATCAATTTAGTCATAGATGATGAATTGTCTGGTTCAATATCTTTGCAATTGCAAAATACAACATTACTTCAATTATTA
AAATCTATCGCTGAAATAAAACATTTGCGGTTTTGGGAAAATAATGGGATATATTATTTAAGTCAGTCGCAAGCAACTAA
TCAAGAAAATGTAGTGTTAAATTCATCAACAGACATAGCAACATCAGCAGATATGTTAATAACTTCAATAAAGTTGCACT
ATGCCAAAGCATCCGAGGTAATGAAAGCCCTCACTTCAGGGAATGGATCTTTATTAACAGCAAATGGAAGTATAAGTTTT
GATGAACGCAATAATTTACTCATTATCCAAGATGAAGCTAAAAAAATAAAACAAATTAGGAAATTGGTAGCGGATATGGA
TAAACCTATTCAGCAAATTATTATTGAAGCTCGTATTGTCACAATTAGCGATGAAAGTTTAAAAGAATTGGGCGTTAGAT
GGGGAATTTTTTCTCCCTCAGATATAAGCCATAGCGTTTCTGGAAGCTTAAATGCAAATGGATTCTCAAATATTACGGAT
AATCTAAATATTAATTTTGCGACGAGTTCAAATCCTGCAGGTTCGGTAGCTTTGCAAGTAGCTAAAATTAATGGAAGGTT
ATTAGATTTAGAGTTGACAGCATTAGAAAGAGAAAATAATGTTGAAATTATTGCGAGTCCACGATTATTGACCACAAATA
AGAAAAGTGCAAGTATCAAGCAAGGTACAGAAATCCCCTATGTAGTGAATAATGGAAAAAATGATATGCAGTCTATTGAA
TTCCGAGAAGCTGTACTTGGGCTTGAAGTTACACCTCATTTATTAAAGCATAACGGTATTTTACTTGATCTTGTTGTTAG
TCAGAATGCACCTGGAGGACGAGTATCTTATGGAGAAAATGAGGTGATTTCTATTGATAAACAGGAAATTAATACGCAAG
TCTTTGCACAAGACGGCGAAACGATTGTATTGGGCGGAATTTTCCACGATACGATTACTAAAGCACTTGATAAAGTACCA
ATTTTAGGAGACATACCTGGAATAAAAAGGCTATTTAGTAAGGAAGTTGAGAGACATCAAAAGAGGGAACTCGTTATTTT
TGTTACACCACGCTTAATTCGTAGTGGTACAGTAGAGGAATGA

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(274-434)

Bacterial type II/III secretion system short domain (Secretin_N)

(129-192)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comE Haemophilus influenzae Rd KW20

64.419

97.727

0.63
  comE Haemophilus influenzae 86-028NP

65.707

94.773

0.623
  comE Glaesserella parasuis strain SC1401

49.883

97.045

0.484
  pilQ Vibrio campbellii strain DS40M4

39.765

96.591

0.384
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

40.288

94.773

0.382
  pilQ Vibrio cholerae strain A1552

40.288

94.773

0.382

Multiple sequence alignment