Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA   Type   Machinery gene
Locus tag   ACIQTD_RS05330 Genome accession   NZ_CP173185
Coordinates   1071840..1073162 (-) Length   440 a.a.
NCBI ID   WP_058212250.1    Uniprot ID   A0AAW5TRR8
Organism   Lactococcus lactis subsp. lactis strain MGEL24009     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1066840..1078162
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACIQTD_RS05300 - 1067983..1068951 (+) 969 WP_003130649.1 PhoH family protein -
  ACIQTD_RS05305 - 1069009..1069488 (+) 480 WP_201247958.1 NUDIX hydrolase -
  ACIQTD_RS05310 ybeY 1069640..1070128 (+) 489 WP_021722516.1 rRNA maturation RNase YbeY -
  ACIQTD_RS05315 - 1070112..1070567 (+) 456 WP_003130653.1 diacylglycerol kinase family protein -
  ACIQTD_RS05320 - 1070581..1071159 (+) 579 WP_270250167.1 nucleotidyltransferase family protein -
  ACIQTD_RS05325 comFC 1071193..1071597 (-) 405 WP_003130657.1 ComF family protein Machinery gene
  ACIQTD_RS05330 comFA 1071840..1073162 (-) 1323 WP_058212250.1 DEAD/DEAH box helicase Machinery gene
  ACIQTD_RS05335 - 1073219..1073848 (+) 630 WP_003130660.1 YigZ family protein -
  ACIQTD_RS05340 - 1073982..1074479 (+) 498 WP_012897708.1 VanZ family protein -
  ACIQTD_RS05345 - 1074527..1075546 (+) 1020 WP_012897709.1 DUF475 domain-containing protein -
  ACIQTD_RS05350 - 1075662..1076408 (+) 747 WP_320718947.1 bifunctional metallophosphatase/5'-nucleotidase -
  ACIQTD_RS05355 glyQ 1076694..1077647 (+) 954 WP_003130667.1 glycine--tRNA ligase subunit alpha -

Sequence

Protein


Download         Length: 440 a.a.        Molecular weight: 50192.71 Da        Isoelectric Point: 10.1065

>NTDB_id=1070495 ACIQTD_RS05330 WP_058212250.1 1071840..1073162(-) (comFA) [Lactococcus lactis subsp. lactis strain MGEL24009]
MSTNQEKLFGRLLLKNDILQLIKSTDKISVSKIFSNFLLEAKVNPILGMTSISSNKIKCNRCGTVHIKNSVKLPIGVFYC
PSCIQLGRVRSDEFLYFLPQKNFPKKSYINWSGKLTENQKSISNALCQEINSHQQIIVQAVTGAGKTEMIYQVIEQILES
GGVVGLASPRIDVCLELHQRLSRDFSCKIPLLYHDGDSYFRAPLIIMTSHQLLRFKEAFDLLIIDEVDAFPFRDNEMLYF
AAEKARKIEGNLIYLTATSTDKLEKDIKKQKLYPLFLPRRFHNFPLVVPKFFWKNKFDKKLIEQRNSGFPLLIFAAEIEF
GQEFAKQLQLKFPKEKIASVASTTKDRLEIVKAFRNKEITILIATSILERGVTFPNVDVFVINSEHPNFTKSALIQMAGR
VGRSPERPTGLVSFFHYGKSKAMCQAVREIKKMNQLGGFS

Nucleotide


Download         Length: 1323 bp        

>NTDB_id=1070495 ACIQTD_RS05330 WP_058212250.1 1071840..1073162(-) (comFA) [Lactococcus lactis subsp. lactis strain MGEL24009]
ATGAGCACTAATCAAGAAAAGTTATTTGGCCGTTTATTATTAAAAAATGATATTTTACAACTTATAAAAAGTACTGACAA
GATTTCTGTCAGTAAAATTTTTAGTAATTTTTTGTTAGAAGCGAAGGTGAATCCAATTTTGGGAATGACTTCAATTTCTT
CCAATAAAATAAAATGCAACCGTTGTGGGACTGTTCATATAAAAAATTCTGTCAAACTTCCAATTGGTGTATTTTACTGT
CCAAGTTGTATTCAATTAGGTCGAGTCCGCTCCGATGAATTCTTGTACTTTCTGCCACAAAAGAATTTCCCAAAGAAATC
ATATATAAACTGGTCGGGAAAACTGACAGAGAATCAAAAATCAATTTCAAATGCCCTCTGTCAGGAAATTAATTCTCATC
AGCAAATAATTGTCCAAGCTGTGACTGGAGCTGGAAAAACTGAAATGATTTATCAAGTCATTGAGCAAATTTTAGAAAGC
GGTGGGGTTGTTGGTCTAGCTAGTCCAAGAATTGATGTTTGTCTTGAACTTCATCAGCGATTATCACGTGATTTTTCCTG
TAAGATTCCACTCTTATATCATGATGGCGACAGCTATTTCCGAGCTCCATTAATAATAATGACCAGTCATCAGCTTTTAC
GTTTCAAGGAAGCTTTTGATTTGCTGATTATTGATGAAGTTGATGCCTTTCCCTTTAGAGATAATGAAATGCTTTATTTT
GCGGCAGAAAAAGCAAGAAAAATAGAAGGAAATTTAATATATTTGACCGCAACTTCTACTGACAAACTTGAAAAAGATAT
AAAAAAGCAAAAACTCTATCCTTTGTTTCTCCCGCGTCGTTTTCACAATTTTCCTTTAGTCGTGCCTAAATTTTTTTGGA
AAAATAAATTTGATAAGAAATTAATTGAGCAAAGAAATAGTGGCTTTCCTCTTCTTATTTTTGCTGCTGAAATTGAATTT
GGACAAGAATTTGCAAAACAACTACAATTGAAATTTCCTAAAGAAAAAATTGCTTCCGTTGCTTCAACAACAAAAGATAG
ATTGGAAATTGTTAAAGCTTTTAGAAATAAAGAAATTACTATTTTAATAGCGACTTCAATTCTTGAACGGGGAGTCACTT
TTCCAAATGTTGATGTTTTTGTCATCAACAGTGAGCACCCAAACTTCACTAAATCCGCACTGATACAAATGGCTGGACGT
GTGGGTCGTAGTCCTGAACGTCCAACAGGCTTAGTTAGTTTTTTTCATTACGGAAAATCCAAAGCAATGTGTCAGGCAGT
TAGAGAAATCAAAAAAATGAACCAACTGGGAGGTTTTTCATGA

Domains

Predicted by InterproScan.

Helicase conserved C-terminal domain (Helicase_C)

(300-403)

Type III restriction enzyme, res subunit (ResIII)

(114-232)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA Lactococcus lactis subsp. cremoris KW2

74.75

90.909

0.68
  comFA/cflA Streptococcus mitis SK321

52.01

90.455

0.47
  comFA/cflA Streptococcus pneumoniae Rx1

52.01

90.455

0.47
  comFA/cflA Streptococcus pneumoniae D39

52.01

90.455

0.47
  comFA/cflA Streptococcus pneumoniae R6

52.01

90.455

0.47
  comFA/cflA Streptococcus pneumoniae TIGR4

52.01

90.455

0.47
  comFA/cflA Streptococcus mitis NCTC 12261

51.256

90.455

0.464
  comFA Bacillus subtilis subsp. subtilis str. 168

37.251

100

0.382

Multiple sequence alignment