Detailed information NTG1070403

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: ACINK7_RS20345
• Genome accession: NZ_CP173170
• Coordinates: 4665984..4667147 (+)
• Length: 387 a.a.
• NCBI ID: WP_404358168.1
• Uniprot ID: -
• Organism: Methylotuvimicrobium sp. KM1
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4660984..4672147

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACINK7_RS20325 (ACINK7_20325)	argJ	4662012..4663226 (-)	1215	WP_404358162.1	bifunctional glutamate N-acetyltransferase/amino-acid acetyltransferase ArgJ	-
ACINK7_RS20330 (ACINK7_20330)	-	4663237..4664379 (-)	1143	WP_404358164.1	cation diffusion facilitator family transporter	-
ACINK7_RS20335 (ACINK7_20335)	-	4664379..4664606 (-)	228	WP_404358166.1	sulfurtransferase TusA family protein	-
ACINK7_RS20340 (ACINK7_20340)	-	4664603..4665646 (-)	1044	WP_125218047.1	tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase	-
ACINK7_RS20345 (ACINK7_20345)	pilU	4665984..4667147 (+)	1164	WP_404358168.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
ACINK7_RS20350 (ACINK7_20350)	-	4667535..4668026 (-)	492	WP_404358170.1	hypothetical protein	-
ACINK7_RS20355 (ACINK7_20355)	-	4668035..4668202 (-)	168	WP_404358172.1	DUF3641 domain-containing protein	-
ACINK7_RS20360 (ACINK7_20360)	-	4668511..4670850 (+)	2340	WP_404358174.1	alpha/beta fold hydrolase	-
ACINK7_RS20365 (ACINK7_20365)	-	4670847..4671989 (+)	1143	WP_404358177.1	thiolase family protein	-

Sequence

Protein

• Length: 387 a.a.
• Molecular weight: 42841.12 Da
• Isoelectric Point: 6.2952

>NTDB_id=1070403 ACINK7_RS20345 WP_404358168.1 4665984..4667147(+) (pilU) [Methylotuvimicrobium sp. KM1]
MDFKDYLKILVSKDGSDLYLTVGAPAAAKFQGALKPLESEKLTGERIKAIAYNLMDAEQQKEFEHVPEMNLAITEPGIGR
FRINIFKQRNNLALVIRNIKVDIPNADQLGLPEVLKKVIMEKRGLILFVGGTGSGKSTSLAALIDHRNSNSSGHIITIED
PIEYLHPHKRCLVNQREVGVDTLSYEDALKNTLRQAPDVILIGEIRSQETMEHALAFAETGHLCLSTLHANNANQALDRI
INFFPEERRNQLLLDLSLNLKAFVSQRLVPTVDGKRVAAIEILLGTQLVKDLIHKGEIHAIKEAMEKSENIGMQTFDSHL
LKLFKEGKISLEEALQNSDSPNNLKLKINLSEGKGSATEAVAKSPGGLTSRFSLEAIEKDQEEEGQS

Nucleotide

• Length: 1164 bp

>NTDB_id=1070403 ACINK7_RS20345 WP_404358168.1 4665984..4667147(+) (pilU) [Methylotuvimicrobium sp. KM1]
ATGGATTTTAAAGATTATCTAAAAATTCTAGTCAGCAAAGACGGCTCCGATCTTTACTTAACGGTCGGCGCGCCGGCCGC
GGCCAAATTTCAAGGCGCATTGAAACCGCTCGAAAGCGAAAAACTGACCGGCGAACGCATTAAGGCTATCGCCTACAACC
TGATGGATGCCGAGCAGCAAAAAGAATTCGAACACGTCCCGGAAATGAATCTGGCGATCACCGAACCCGGCATCGGCCGC
TTCCGCATCAATATTTTCAAACAGCGCAATAACCTGGCCTTGGTCATTCGAAATATCAAGGTCGATATACCGAATGCCGA
TCAATTAGGTCTGCCTGAAGTGCTGAAAAAAGTGATCATGGAAAAGCGCGGCTTGATTCTATTCGTCGGCGGCACCGGTT
CCGGCAAATCGACCTCGCTAGCCGCACTGATCGACCACCGCAACAGCAATTCGTCGGGGCACATCATCACGATCGAAGAC
CCGATCGAATACCTTCATCCGCACAAACGCTGTCTAGTCAATCAACGCGAAGTCGGCGTCGATACGCTCAGTTACGAGGA
CGCGTTGAAAAACACGCTACGCCAGGCACCGGACGTGATTCTGATCGGCGAAATCCGCTCTCAAGAAACGATGGAACACG
CGCTCGCCTTCGCCGAAACCGGGCATTTATGTTTGTCGACGCTGCACGCCAACAACGCCAATCAGGCTCTGGACCGCATT
ATCAATTTTTTTCCGGAAGAACGCCGCAATCAATTATTGCTGGATCTATCGCTGAATTTGAAAGCCTTCGTCTCGCAGCG
ACTGGTTCCTACCGTGGACGGCAAACGGGTCGCCGCGATTGAAATCTTGTTGGGGACGCAATTGGTCAAAGACTTGATTC
ATAAAGGCGAAATTCATGCGATCAAGGAAGCGATGGAAAAATCCGAAAATATCGGCATGCAAACCTTCGACAGCCACTTG
CTCAAGCTTTTCAAGGAAGGCAAAATTTCGCTCGAAGAAGCTTTGCAAAACTCCGACTCGCCGAATAACTTAAAATTGAA
GATCAACCTCAGCGAAGGCAAGGGTTCGGCGACTGAAGCGGTCGCTAAATCGCCTGGCGGACTGACTTCAAGATTCTCGC
TTGAGGCGATTGAAAAGGATCAGGAGGAAGAGGGGCAAAGCTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (68-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	56	96.899	0.543
pilU	Acinetobacter baylyi ADP1	53.672	91.473	0.491
pilU	Vibrio cholerae strain A1552	50.847	91.473	0.465
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	40.872	94.832	0.388