Detailed information NTG1069828

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: PA7077_RS20660
• Genome accession: NZ_CP173020
• Coordinates: 4445374..4446522 (-)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain PA7077
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4440374..4451522

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PA7077_RS20640	-	4441111..4442296 (-)	1186	Protein_4075	cystathionine gamma-synthase	-
PA7077_RS20645 (PA7077_04110)	-	4442293..4443666 (-)	1374	WP_003100955.1	pyridoxal-phosphate dependent enzyme	-
PA7077_RS20650 (PA7077_04111)	-	4443909..4444313 (-)	405	WP_003084560.1	NINE protein	-
PA7077_RS20655 (PA7077_04112)	czcD	4444467..4445366 (+)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
PA7077_RS20660 (PA7077_04113)	pilU	4445374..4446522 (-)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
PA7077_RS20665 (PA7077_04114)	pilT	4446700..4447734 (-)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
PA7077_RS20670 (PA7077_04115)	-	4447949..4448641 (+)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
PA7077_RS20675 (PA7077_04116)	proC	4448653..4449474 (+)	822	WP_003114890.1	pyrroline-5-carboxylate reductase	-
PA7077_RS20680 (PA7077_04117)	-	4449485..4450078 (+)	594	WP_003084544.1	YggT family protein	-
PA7077_RS20685	-	4450075..4450371 (+)	297	WP_031655952.1	DUF167 domain-containing protein	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=1069828 PA7077_RS20660 WP_003100959.1 4445374..4446522(-) (pilU) [Pseudomonas aeruginosa strain PA7077]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=1069828 PA7077_RS20660 WP_003100959.1 4445374..4446522(-) (pilU) [Pseudomonas aeruginosa strain PA7077]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382