Detailed information NTG1069786

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: PA7078_RS06595
• Genome accession: NZ_CP173019
• Coordinates: 1441173..1442321 (+)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain PA7078
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1436173..1447321

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PA7078_RS06570	-	1437324..1437620 (-)	297	WP_031655952.1	DUF167 domain-containing protein	-
PA7078_RS06575 (PA7078_01313)	-	1437617..1438210 (-)	594	WP_003084544.1	YggT family protein	-
PA7078_RS06580 (PA7078_01314)	proC	1438221..1439042 (-)	822	WP_003114890.1	pyrroline-5-carboxylate reductase	-
PA7078_RS06585 (PA7078_01315)	-	1439054..1439746 (-)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
PA7078_RS06590 (PA7078_01316)	pilT	1439961..1440995 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
PA7078_RS06595 (PA7078_01317)	pilU	1441173..1442321 (+)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
PA7078_RS06600 (PA7078_01318)	czcD	1442329..1443228 (-)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
PA7078_RS06605 (PA7078_01319)	-	1443382..1443786 (+)	405	WP_003084560.1	NINE protein	-
PA7078_RS06610 (PA7078_01320)	-	1444029..1445402 (+)	1374	WP_003100955.1	pyridoxal-phosphate dependent enzyme	-
PA7078_RS06615 (PA7078_01321)	-	1445399..1446583 (+)	1185	WP_003100953.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=1069786 PA7078_RS06595 WP_003100959.1 1441173..1442321(+) (pilU) [Pseudomonas aeruginosa strain PA7078]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=1069786 PA7078_RS06595 WP_003100959.1 1441173..1442321(+) (pilU) [Pseudomonas aeruginosa strain PA7078]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382