Detailed information NTG1069664

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: ACIJUN_RS24250
• Genome accession: NZ_CP172962
• Coordinates: 5204788..5205936 (+)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain YB1
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 5199788..5210936

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACIJUN_RS24225 (ACIJUN_24230)	-	5200939..5201235 (-)	297	WP_031628111.1	DUF167 domain-containing protein	-
ACIJUN_RS24230 (ACIJUN_24235)	-	5201232..5201825 (-)	594	WP_003084544.1	YggT family protein	-
ACIJUN_RS24235 (ACIJUN_24240)	proC	5201836..5202657 (-)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
ACIJUN_RS24240 (ACIJUN_24245)	-	5202669..5203361 (-)	693	WP_023464234.1	YggS family pyridoxal phosphate-dependent enzyme	-
ACIJUN_RS24245 (ACIJUN_24250)	pilT	5203576..5204610 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
ACIJUN_RS24250 (ACIJUN_24255)	pilU	5204788..5205936 (+)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
ACIJUN_RS24255 (ACIJUN_24260)	czcD	5205944..5206843 (-)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
ACIJUN_RS24260 (ACIJUN_24265)	-	5206997..5207401 (+)	405	WP_003084560.1	NINE protein	-
ACIJUN_RS24265 (ACIJUN_24270)	-	5207644..5209017 (+)	1374	WP_003100955.1	pyridoxal-phosphate dependent enzyme	-
ACIJUN_RS24270 (ACIJUN_24275)	-	5209014..5210198 (+)	1185	WP_003110017.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=1069664 ACIJUN_RS24250 WP_003100959.1 5204788..5205936(+) (pilU) [Pseudomonas aeruginosa strain YB1]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=1069664 ACIJUN_RS24250 WP_003100959.1 5204788..5205936(+) (pilU) [Pseudomonas aeruginosa strain YB1]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382