Detailed information NTG1069505

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: ACFELJ_RS02080
• Genome accession: NZ_CP172859
• Coordinates: 449460..450608 (+)
• Length: 382 a.a.
• NCBI ID: WP_058018263.1
• Uniprot ID: -
• Organism: Pseudomonas aeruginosa strain 238629E_ID006
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 444460..455608

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACFELJ_RS02055 (ACFELJ_02055)	-	445611..445907 (-)	297	WP_031628111.1	DUF167 domain-containing protein	-
ACFELJ_RS02060 (ACFELJ_02060)	-	445904..446497 (-)	594	WP_003084544.1	YggT family protein	-
ACFELJ_RS02065 (ACFELJ_02065)	proC	446508..447329 (-)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
ACFELJ_RS02070 (ACFELJ_02070)	-	447341..448033 (-)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
ACFELJ_RS02075 (ACFELJ_02075)	pilT	448248..449282 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
ACFELJ_RS02080 (ACFELJ_02080)	pilU	449460..450608 (+)	1149	WP_058018263.1	type IV pilus ATPase PilU	Machinery gene
ACFELJ_RS02085 (ACFELJ_02085)	czcD	450616..451515 (-)	900	WP_400637706.1	CDF family zinc transporter CzcD	-
ACFELJ_RS02090 (ACFELJ_02090)	-	451669..452073 (+)	405	WP_003084560.1	NINE protein	-
ACFELJ_RS02095 (ACFELJ_02095)	-	452315..453688 (+)	1374	WP_004365040.1	pyridoxal-phosphate dependent enzyme	-
ACFELJ_RS02100 (ACFELJ_02100)	-	453685..454869 (+)	1185	WP_033972291.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42544.86 Da
• Isoelectric Point: 6.7792

>NTDB_id=1069505 ACFELJ_RS02080 WP_058018263.1 449460..450608(+) (pilU) [Pseudomonas aeruginosa strain 238629E_ID006]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYIQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=1069505 ACFELJ_RS02080 WP_058018263.1 449460..450608(+) (pilU) [Pseudomonas aeruginosa strain 238629E_ID006]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGTTGTCGCCGGAACAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGACTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTTTTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGATTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCATG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACATCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCCGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterProScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382