Detailed information NTG1069369

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: ACFELK_RS02020
• Genome accession: NZ_CP172855
• Coordinates: 432544..433692 (+)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain 238633E_ID012
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 427544..438692

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACFELK_RS01995 (ACFELK_01995)	-	428695..428991 (-)	297	WP_031628111.1	DUF167 domain-containing protein	-
ACFELK_RS02000 (ACFELK_02000)	-	428988..429581 (-)	594	WP_003155373.1	YggT family protein	-
ACFELK_RS02005 (ACFELK_02005)	proC	429592..430413 (-)	822	WP_003142635.1	pyrroline-5-carboxylate reductase	-
ACFELK_RS02010 (ACFELK_02010)	-	430425..431117 (-)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
ACFELK_RS02015 (ACFELK_02015)	pilT	431332..432366 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
ACFELK_RS02020 (ACFELK_02020)	pilU	432544..433692 (+)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
ACFELK_RS02025 (ACFELK_02025)	czcD	433700..434599 (-)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
ACFELK_RS02030 (ACFELK_02030)	-	434753..435157 (+)	405	WP_003084560.1	NINE protein	-
ACFELK_RS02035 (ACFELK_02035)	-	435400..436773 (+)	1374	WP_003160915.1	pyridoxal-phosphate dependent enzyme	-
ACFELK_RS02040 (ACFELK_02040)	-	436770..437954 (+)	1185	WP_003110017.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=1069369 ACFELK_RS02020 WP_003100959.1 432544..433692(+) (pilU) [Pseudomonas aeruginosa strain 238633E_ID012]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=1069369 ACFELK_RS02020 WP_003100959.1 432544..433692(+) (pilU) [Pseudomonas aeruginosa strain 238633E_ID012]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGTCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382