Detailed information NTG1069362

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: ACFELD_RS27380
• Genome accession: NZ_CP172854
• Coordinates: 5872962..5874026 (-)
• Length: 354 a.a.
• NCBI ID: WP_003110888.1
• Uniprot ID: -
• Organism: Pseudomonas aeruginosa strain 238634E_ID013
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 5867962..5879026

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACFELD_RS27355 (ACFELD_27355)	aroK	5868497..5869015 (-)	519	WP_003095833.1	shikimate kinase AroK	-
ACFELD_RS27360 (ACFELD_27360)	pilQ	5869027..5871171 (-)	2145	WP_003114575.1	type 4a pilus secretin PilQ	Machinery gene
ACFELD_RS27365 (ACFELD_27365)	pilP	5871225..5871749 (-)	525	WP_003095836.1	type 4a pilus biogenesis lipoprotein PilP	-
ACFELD_RS27370 (ACFELD_27370)	pilO	5871746..5872369 (-)	624	WP_003103268.1	type 4a pilus biogenesis protein PilO	-
ACFELD_RS27375 (ACFELD_27375)	pilN	5872366..5872962 (-)	597	WP_003095839.1	type 4a pilus biogenesis protein PilN	-
ACFELD_RS27380 (ACFELD_27380)	pilM	5872962..5874026 (-)	1065	WP_003110888.1	type IV pilus assembly protein PilM	Machinery gene
ACFELD_RS27385 (ACFELD_27385)	-	5874211..5876682 (+)	2472	WP_003095841.1	penicillin-binding protein 1A	-
ACFELD_RS27390 (ACFELD_27390)	-	5876787..5878055 (-)	1269	WP_003103278.1	malic enzyme	-

Sequence

Protein

• Length: 354 a.a.
• Molecular weight: 37983.48 Da
• Isoelectric Point: 4.5405

>NTDB_id=1069362 ACFELD_RS27380 WP_003110888.1 5872962..5874026(-) (pilM) [Pseudomonas aeruginosa strain 238634E_ID013]
MLGLIKKKANTLLGIDISSTSVKLLELSRSGGRYKVEAYAVEPLPPNAVVEKNIVELEGVGQALSRVLVKAKTNLKSAVV
AVAGSAVITKTIEMEAGLSEDELENQLKIEADQYIPYPLEEVAIDFEVQGLSARNPERVDVLLAACRKENVEVREAALAL
AGLTAKVVDVEAYALERSYALLSSQLGADTDQLTVAVVDIGATMTTLSVLHNGRTIYTREQLFGGRQLTEEIQRRYGLSV
EEAGLAKKQGGLPDDYDSEVLRPFKDAVVQQVSRSLQFFFAAGQFNDVDYIVLAGGTASIQDLDRLIQQKIGTPTLVANP
FADMALNGKVNAGALASDAPALMIACGLALRSFD

Nucleotide

• Length: 1065 bp

>NTDB_id=1069362 ACFELD_RS27380 WP_003110888.1 5872962..5874026(-) (pilM) [Pseudomonas aeruginosa strain 238634E_ID013]
GTGCTAGGGCTCATAAAGAAGAAAGCGAACACCTTGCTGGGGATCGACATCAGCTCGACCTCGGTAAAGCTCCTTGAGTT
GAGCCGCTCCGGAGGCCGCTACAAAGTGGAGGCCTATGCCGTCGAGCCGCTTCCGCCGAATGCAGTCGTGGAAAAGAACA
TCGTCGAGCTGGAAGGGGTCGGCCAGGCGTTGTCACGGGTGCTGGTCAAGGCGAAAACCAACCTGAAGTCGGCCGTGGTC
GCCGTCGCCGGTTCGGCGGTGATCACCAAGACCATCGAGATGGAGGCCGGGCTTTCCGAGGATGAACTGGAAAACCAGCT
GAAGATCGAGGCCGACCAGTACATCCCCTATCCGCTGGAGGAAGTCGCCATCGACTTCGAGGTCCAGGGGCTCTCCGCGC
GCAATCCCGAGCGCGTGGACGTACTCCTGGCCGCCTGTCGGAAAGAAAACGTCGAGGTTCGCGAAGCGGCCCTGGCGCTG
GCCGGCCTGACCGCCAAGGTGGTCGACGTCGAGGCCTACGCGCTGGAGCGCTCCTATGCGCTGCTGAGCAGCCAGCTGGG
TGCCGACACCGACCAGTTGACCGTGGCGGTAGTGGACATCGGCGCGACCATGACCACCCTGAGCGTCCTGCACAACGGAC
GCACCATCTATACCCGCGAACAGCTGTTCGGCGGTCGCCAGCTCACCGAGGAGATCCAGCGGCGTTACGGACTCTCGGTG
GAGGAAGCCGGTCTCGCCAAGAAGCAGGGCGGTCTTCCGGATGACTACGACAGCGAAGTTCTGCGTCCGTTCAAGGACGC
CGTGGTGCAGCAGGTTTCCCGCTCGCTGCAGTTCTTCTTCGCCGCCGGACAGTTCAACGACGTCGACTACATCGTCCTGG
CGGGCGGTACGGCGTCTATCCAGGATCTCGATCGGTTGATCCAGCAGAAGATCGGCACCCCGACCCTGGTCGCCAACCCG
TTCGCCGACATGGCGCTGAACGGCAAGGTGAATGCCGGCGCCCTGGCCAGCGACGCTCCGGCGTTGATGATCGCCTGCGG
CCTGGCGTTGAGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-353)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	55.899	100	0.562
comM	Acinetobacter nosocomialis M2	55.899	100	0.562
comM	Acinetobacter baylyi ADP1	51.977	100	0.52
pilM	Legionella pneumophila strain ERS1305867	46.61	100	0.466