Detailed information NTG1069263

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: ACFELM_RS02015
• Genome accession: NZ_CP172852
• Coordinates: 432435..433583 (+)
• Length: 382 a.a.
• NCBI ID: WP_003110016.1
• Uniprot ID: A0A0H2ZL88
• Organism: Pseudomonas aeruginosa strain 238636E_ID015
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 427435..438583

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACFELM_RS01990 (ACFELM_01990)	-	428586..428882 (-)	297	WP_031628111.1	DUF167 domain-containing protein	-
ACFELM_RS01995 (ACFELM_01995)	-	428879..429472 (-)	594	WP_003084544.1	YggT family protein	-
ACFELM_RS02000 (ACFELM_02000)	proC	429483..430304 (-)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
ACFELM_RS02005 (ACFELM_02005)	-	430316..431008 (-)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
ACFELM_RS02010 (ACFELM_02010)	pilT	431223..432257 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
ACFELM_RS02015 (ACFELM_02015)	pilU	432435..433583 (+)	1149	WP_003110016.1	type IV pilus ATPase PilU	Machinery gene
ACFELM_RS02020 (ACFELM_02020)	czcD	433591..434490 (-)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
ACFELM_RS02025 (ACFELM_02025)	-	434644..435048 (+)	405	WP_003084560.1	NINE protein	-
ACFELM_RS02030 (ACFELM_02030)	-	435291..436664 (+)	1374	WP_003100955.1	pyridoxal-phosphate dependent enzyme	-
ACFELM_RS02035 (ACFELM_02035)	-	436661..437845 (+)	1185	WP_003110017.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42559.83 Da
• Isoelectric Point: 6.7792

>NTDB_id=1069263 ACFELM_RS02015 WP_003110016.1 432435..433583(+) (pilU) [Pseudomonas aeruginosa strain 238636E_ID015]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSNLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=1069263 ACFELM_RS02015 WP_003110016.1 432435..433583(+) (pilU) [Pseudomonas aeruginosa strain 238636E_ID015]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAACCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: A0A0H2ZL88

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.22	100	0.882
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382