Detailed information NTG1069256

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: ACFELF_RS27535
• Genome accession: NZ_CP172851
• Coordinates: 5897097..5898161 (-)
• Length: 354 a.a.
• NCBI ID: WP_023103573.1
• Uniprot ID: -
• Organism: Pseudomonas aeruginosa strain 238637E_ID016
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 5892097..5903161

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACFELF_RS27510 (ACFELF_27510)	aroK	5892644..5893162 (-)	519	WP_003095833.1	shikimate kinase AroK	-
ACFELF_RS27515 (ACFELF_27515)	pilQ	5893174..5895306 (-)	2133	WP_003116137.1	type 4a pilus secretin PilQ	Machinery gene
ACFELF_RS27520 (ACFELF_27520)	pilP	5895360..5895884 (-)	525	WP_003116136.1	type 4a pilus biogenesis lipoprotein PilP	-
ACFELF_RS27525 (ACFELF_27525)	pilO	5895881..5896504 (-)	624	WP_003116135.1	type 4a pilus biogenesis protein PilO	-
ACFELF_RS27530 (ACFELF_27530)	pilN	5896501..5897097 (-)	597	WP_003141783.1	type 4a pilus biogenesis protein PilN	-
ACFELF_RS27535 (ACFELF_27535)	pilM	5897097..5898161 (-)	1065	WP_023103573.1	type IV pilus assembly protein PilM	Machinery gene
ACFELF_RS27540 (ACFELF_27540)	-	5898346..5900817 (+)	2472	WP_003095841.1	penicillin-binding protein 1A	-
ACFELF_RS27545 (ACFELF_27545)	-	5900922..5902190 (-)	1269	WP_003103278.1	malic enzyme	-

Sequence

Protein

• Length: 354 a.a.
• Molecular weight: 37964.43 Da
• Isoelectric Point: 4.5399

>NTDB_id=1069256 ACFELF_RS27535 WP_023103573.1 5897097..5898161(-) (pilM) [Pseudomonas aeruginosa strain 238637E_ID016]
MLGLIKKKANTLLGIDISSTSVKLLELSRSGGRYKVEAYAVEPLPPNAVVEKNIVELEGVGQALSRVLVKAKTNLKSAVV
AVAGSAVITKTIEMEAGLSEDELENQLKIEADQYIPYPLEEVAIDFEVQGLSARNPEHVDVLLAACRKENVEVREAALAL
AGLTAKVVDVEAYALERSYALLSSQLGADTDQLTVAVVDIGATMTTLSVLHNGRTIYTREQLFGGRQLTEEIQRRYGLSV
EEAGLAKKQGGLPDDYDSEVLRPFKDAVVQQVSRSLQFFFAAGQFNDVDYIVLAGGTASIQDLDRLIQQKIGTPTLVANP
FADMALNGKVNAGALASDAPALMIACGLALRSFD

Nucleotide

• Length: 1065 bp

>NTDB_id=1069256 ACFELF_RS27535 WP_023103573.1 5897097..5898161(-) (pilM) [Pseudomonas aeruginosa strain 238637E_ID016]
GTGCTAGGGCTCATAAAGAAGAAAGCGAACACCTTGCTGGGGATCGACATCAGCTCGACCTCGGTAAAGCTCCTTGAGTT
GAGCCGCTCCGGAGGCCGCTACAAAGTGGAGGCCTATGCCGTCGAGCCGCTTCCGCCGAATGCAGTCGTGGAAAAGAACA
TCGTCGAGCTGGAAGGGGTCGGCCAGGCGTTGTCACGGGTGCTGGTCAAGGCGAAAACCAACCTGAAGTCGGCCGTGGTC
GCCGTCGCCGGTTCGGCGGTGATCACCAAGACCATCGAGATGGAGGCCGGGCTTTCCGAGGATGAACTGGAAAACCAGCT
GAAGATCGAGGCCGACCAGTACATCCCCTATCCGCTGGAGGAAGTCGCCATCGACTTCGAGGTCCAGGGACTCTCCGCGC
GCAATCCCGAGCACGTGGACGTACTCCTGGCCGCCTGTCGGAAAGAGAACGTCGAGGTTCGCGAAGCGGCCCTGGCGCTG
GCCGGCCTGACCGCCAAGGTGGTCGACGTCGAGGCCTACGCGCTGGAGCGCTCCTATGCGCTGCTGAGCAGCCAGCTGGG
TGCCGACACTGACCAGTTGACCGTGGCGGTAGTGGACATCGGCGCGACCATGACCACCCTGAGCGTCCTGCACAACGGAC
GCACCATCTATACCCGCGAACAGCTGTTCGGCGGTCGCCAGCTCACCGAGGAGATCCAGCGGCGTTACGGACTCTCGGTG
GAAGAAGCCGGTCTCGCCAAGAAGCAGGGCGGTCTTCCGGATGACTACGACAGCGAAGTTCTGCGTCCGTTCAAGGACGC
CGTGGTGCAGCAGGTTTCCCGCTCGCTGCAGTTCTTCTTCGCCGCCGGACAGTTCAACGACGTCGACTACATCGTCCTGG
CGGGCGGTACGGCGTCTATCCAGGATCTCGATCGGTTGATCCAGCAGAAGATCGGCACCCCGACCCTGGTCGCCAACCCG
TTCGCCGACATGGCGCTGAACGGCAAGGTGAATGCCGGCGCCCTGGCCAGCGACGCTCCGGCGTTGATGATCGCCTGCGG
CCTGGCGTTGAGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-353)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	55.618	100	0.559
comM	Acinetobacter nosocomialis M2	55.618	100	0.559
comM	Acinetobacter baylyi ADP1	51.695	100	0.517
pilM	Legionella pneumophila strain ERS1305867	46.61	100	0.466