Detailed information NTG1069221

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: ACFELB_RS28145
• Genome accession: NZ_CP172850
• Coordinates: 6051682..6052746 (-)
• Length: 354 a.a.
• NCBI ID: WP_003110888.1
• Uniprot ID: -
• Organism: Pseudomonas aeruginosa strain 238638E_ID017
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 6046682..6057746

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACFELB_RS28120 (ACFELB_28120)	aroK	6047217..6047735 (-)	519	WP_003095833.1	shikimate kinase AroK	-
ACFELB_RS28125 (ACFELB_28125)	pilQ	6047747..6049891 (-)	2145	WP_003146401.1	type 4a pilus secretin PilQ	Machinery gene
ACFELB_RS28130 (ACFELB_28130)	pilP	6049945..6050469 (-)	525	WP_003095836.1	type 4a pilus biogenesis lipoprotein PilP	-
ACFELB_RS28135 (ACFELB_28135)	pilO	6050466..6051089 (-)	624	WP_003103268.1	type 4a pilus biogenesis protein PilO	-
ACFELB_RS28140 (ACFELB_28140)	pilN	6051086..6051682 (-)	597	WP_003095839.1	type 4a pilus biogenesis protein PilN	-
ACFELB_RS28145 (ACFELB_28145)	pilM	6051682..6052746 (-)	1065	WP_003110888.1	type IV pilus assembly protein PilM	Machinery gene
ACFELB_RS28150 (ACFELB_28150)	-	6052931..6055402 (+)	2472	WP_003095841.1	penicillin-binding protein 1A	-
ACFELB_RS28155 (ACFELB_28155)	-	6055507..6056775 (-)	1269	WP_003103278.1	malic enzyme	-

Sequence

Protein

• Length: 354 a.a.
• Molecular weight: 37983.48 Da
• Isoelectric Point: 4.5405

>NTDB_id=1069221 ACFELB_RS28145 WP_003110888.1 6051682..6052746(-) (pilM) [Pseudomonas aeruginosa strain 238638E_ID017]
MLGLIKKKANTLLGIDISSTSVKLLELSRSGGRYKVEAYAVEPLPPNAVVEKNIVELEGVGQALSRVLVKAKTNLKSAVV
AVAGSAVITKTIEMEAGLSEDELENQLKIEADQYIPYPLEEVAIDFEVQGLSARNPERVDVLLAACRKENVEVREAALAL
AGLTAKVVDVEAYALERSYALLSSQLGADTDQLTVAVVDIGATMTTLSVLHNGRTIYTREQLFGGRQLTEEIQRRYGLSV
EEAGLAKKQGGLPDDYDSEVLRPFKDAVVQQVSRSLQFFFAAGQFNDVDYIVLAGGTASIQDLDRLIQQKIGTPTLVANP
FADMALNGKVNAGALASDAPALMIACGLALRSFD

Nucleotide

• Length: 1065 bp

>NTDB_id=1069221 ACFELB_RS28145 WP_003110888.1 6051682..6052746(-) (pilM) [Pseudomonas aeruginosa strain 238638E_ID017]
GTGCTAGGGCTCATAAAGAAGAAAGCGAACACCTTGCTGGGGATCGACATCAGCTCGACCTCGGTAAAGCTCCTTGAGTT
GAGCCGCTCCGGAGGCCGCTACAAAGTGGAGGCCTATGCCGTCGAGCCGCTTCCGCCGAATGCAGTCGTGGAAAAGAACA
TCGTCGAGCTGGAAGGGGTCGGCCAGGCGTTGTCACGGGTGCTGGTCAAGGCGAAAACCAACCTGAAGTCGGCCGTGGTC
GCCGTCGCCGGTTCGGCGGTGATCACCAAGACCATCGAGATGGAGGCCGGGCTTTCCGAGGATGAACTGGAAAACCAGCT
GAAGATCGAGGCCGACCAGTACATCCCCTATCCGCTGGAGGAAGTCGCCATCGACTTCGAGGTCCAGGGACTCTCCGCGC
GCAATCCCGAGCGCGTGGACGTACTCCTGGCCGCCTGTCGGAAAGAGAACGTCGAGGTTCGCGAAGCGGCCCTGGCGCTG
GCCGGCCTGACCGCCAAGGTGGTCGACGTCGAGGCCTACGCGCTGGAGCGCTCCTATGCGCTGCTGAGCAGCCAGCTGGG
TGCCGACACCGACCAGTTGACCGTGGCGGTAGTGGACATCGGCGCGACCATGACCACCCTGAGCGTCCTGCACAACGGAC
GCACCATCTATACCCGCGAACAGCTGTTCGGCGGTCGCCAGCTCACCGAGGAGATCCAGCGGCGTTACGGACTCTCGGTG
GAGGAAGCCGGTCTCGCCAAGAAGCAGGGCGGTCTTCCGGATGACTACGACAGCGAAGTTCTGCGTCCGTTCAAGGACGC
CGTGGTGCAGCAGGTTTCCCGCTCGCTGCAGTTCTTCTTCGCCGCCGGACAGTTCAACGACGTCGACTACATCGTCCTGG
CGGGCGGTACGGCGTCTATCCAGGATCTCGATCGGTTGATCCAGCAGAAGATCGGCACCCCGACCCTGGTCGCCAACCCG
TTCGCCGACATGGCGCTGAACGGCAAGGTGAATGCCGGCGCCCTGGCCAGCGACGCTCCGGCGTTGATGATCGCCTGCGG
CCTGGCGTTGAGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-353)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	55.899	100	0.562
comM	Acinetobacter nosocomialis M2	55.899	100	0.562
comM	Acinetobacter baylyi ADP1	51.977	100	0.52
pilM	Legionella pneumophila strain ERS1305867	46.61	100	0.466