Detailed information NTG1069194

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: ACFELB_RS02070
• Genome accession: NZ_CP172850
• Coordinates: 434394..435542 (+)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain 238638E_ID017
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 429394..440542

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACFELB_RS02045 (ACFELB_02045)	-	430545..430841 (-)	297	WP_031628111.1	DUF167 domain-containing protein	-
ACFELB_RS02050 (ACFELB_02050)	-	430838..431431 (-)	594	WP_003084544.1	YggT family protein	-
ACFELB_RS02055 (ACFELB_02055)	proC	431442..432263 (-)	822	WP_003142635.1	pyrroline-5-carboxylate reductase	-
ACFELB_RS02060 (ACFELB_02060)	-	432275..432967 (-)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
ACFELB_RS02065 (ACFELB_02065)	pilT	433182..434216 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
ACFELB_RS02070 (ACFELB_02070)	pilU	434394..435542 (+)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
ACFELB_RS02075 (ACFELB_02075)	czcD	435550..436449 (-)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
ACFELB_RS02080 (ACFELB_02080)	-	436604..437008 (+)	405	WP_003084560.1	NINE protein	-
ACFELB_RS02085 (ACFELB_02085)	-	437251..438624 (+)	1374	WP_003100955.1	pyridoxal-phosphate dependent enzyme	-
ACFELB_RS02090 (ACFELB_02090)	-	438621..439805 (+)	1185	WP_003110017.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=1069194 ACFELB_RS02070 WP_003100959.1 434394..435542(+) (pilU) [Pseudomonas aeruginosa strain 238638E_ID017]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=1069194 ACFELB_RS02070 WP_003100959.1 434394..435542(+) (pilU) [Pseudomonas aeruginosa strain 238638E_ID017]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382