Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilR   Type   Regulator
Locus tag   ACFELC_RS24610 Genome accession   NZ_CP172849
Coordinates   5266359..5267696 (+) Length   445 a.a.
NCBI ID   WP_400564483.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain 238641E_ID020     
Function   regulate pilin expression (predicted from homology)   
Competence regulation

Genomic Context

Location: 5261359..5272696
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACFELC_RS24585 (ACFELC_24585) pgeF 5261502..5262230 (-) 729 WP_400564481.1 peptidoglycan editing factor PgeF -
  ACFELC_RS24590 (ACFELC_24590) rluD 5262227..5263189 (-) 963 WP_033972915.1 23S rRNA pseudouridine(1911/1915/1917) synthase RluD -
  ACFELC_RS24595 (ACFELC_24595) - 5263335..5264360 (+) 1026 WP_003102590.1 outer membrane protein assembly factor BamD -
  ACFELC_RS24600 (ACFELC_24600) - 5264517..5264762 (+) 246 WP_003102591.1 PP0621 family protein -
  ACFELC_RS24605 (ACFELC_24605) pilS 5264752..5266344 (+) 1593 WP_039028118.1 two-component system sensor histidine kinase PilS Regulator
  ACFELC_RS24610 (ACFELC_24610) pilR 5266359..5267696 (+) 1338 WP_400564483.1 two-component system response regulator PilR Regulator
  ACFELC_RS24615 (ACFELC_24615) thiO 5267742..5268836 (-) 1095 WP_033976474.1 glycine oxidase ThiO -
  ACFELC_RS24620 (ACFELC_24620) - 5268971..5269480 (+) 510 WP_033976381.1 Tfp pilus assembly protein FimT/FimU -
  ACFELC_RS24625 (ACFELC_24625) - 5269586..5270092 (+) 507 WP_003134935.1 Tfp pilus assembly protein FimT/FimU -
  ACFELC_RS24630 (ACFELC_24630) pilV 5270083..5270640 (+) 558 WP_003125211.1 type 4a pilus minor pilin PilV Machinery gene
  ACFELC_RS24635 (ACFELC_24635) pilW 5270637..5271461 (+) 825 WP_033976380.1 type 4a pilus minor pilin PilW -
  ACFELC_RS24640 (ACFELC_24640) pilX 5271458..5272045 (+) 588 WP_033972921.1 type 4a pilus minor pilin PilX -

Sequence

Protein


Download         Length: 445 a.a.        Molecular weight: 49753.06 Da        Isoelectric Point: 5.8654

>NTDB_id=1069183 ACFELC_RS24610 WP_400564483.1 5266359..5267696(+) (pilR) [Pseudomonas aeruginosa strain 238641E_ID020]
MSRQKALIVDDEPDIRELLEITLGRMKLDTRSARNVKEARELLAREPFDLCLTDMRLPDGSGLDLVQYIQQRHPQTPVAM
ITAYGSLDTAIQALKAGAFDFLTKPVDLGRLRELVATALRLRNPEAEEAPVDNRLLGESPPMRALRNQIGKLARSQAPVY
ISGESGSGKELVARLIHEQGPRIERPFVPVNCGAIPSELMESEFFGHKKGSFTGAIEDKQGLFQAASGGTLFLDEVADLP
MAMQVKLLRAIQEKAVRAVGGQQEVAVDVRILCATHKDLAAEVGAGRFRQDLYYRLNVIELRVPPLRERREDIPLLAERI
LKRLAGDTGLPAARLTGDAQEKLKNYRFPGNVRELENMLERAYTLCEDDQIQPHDLRLADSPGASQEGAASLSEIDNLED
YLEDIERKLIMQALEETRWNRTAAAQRLGLTFRSMRYRLKKLGID

Nucleotide


Download         Length: 1338 bp        

>NTDB_id=1069183 ACFELC_RS24610 WP_400564483.1 5266359..5267696(+) (pilR) [Pseudomonas aeruginosa strain 238641E_ID020]
ATGAGCCGACAAAAAGCCCTGATCGTCGACGATGAACCGGATATCCGCGAGCTGCTGGAAATCACTCTCGGCCGCATGAA
GCTGGACACCCGCAGCGCCCGCAACGTCAAGGAGGCCCGCGAGTTGCTGGCCCGCGAGCCGTTCGACCTGTGCCTCACCG
ACATGCGCCTGCCGGACGGCAGCGGCCTCGATCTGGTCCAGTACATCCAGCAGCGCCATCCACAGACCCCGGTGGCCATG
ATCACCGCGTACGGCAGCCTGGACACCGCGATCCAGGCGCTCAAGGCCGGTGCCTTCGACTTCCTCACCAAACCGGTCGA
CCTCGGTCGCTTGCGGGAGCTGGTGGCAACCGCACTGCGCCTGCGCAACCCGGAGGCCGAGGAAGCGCCGGTGGACAACC
GCCTGCTCGGCGAATCACCGCCGATGCGCGCCCTGCGCAACCAGATCGGCAAGCTGGCGCGCAGCCAGGCGCCGGTCTAC
ATCAGTGGCGAGTCCGGCAGCGGCAAGGAGCTGGTGGCGCGCCTGATCCATGAGCAGGGGCCACGTATCGAGCGGCCGTT
CGTGCCGGTGAACTGCGGCGCGATTCCCTCCGAACTGATGGAAAGCGAGTTCTTCGGCCACAAGAAAGGCAGCTTCACTG
GCGCTATCGAAGACAAGCAGGGCCTGTTCCAGGCCGCCAGTGGCGGCACCCTGTTCCTCGACGAAGTCGCCGACCTGCCG
ATGGCCATGCAGGTCAAACTGCTCCGAGCGATCCAGGAAAAGGCCGTGCGCGCGGTCGGCGGCCAGCAGGAGGTCGCCGT
CGACGTGCGCATCCTCTGCGCCACCCACAAGGACCTCGCAGCCGAAGTCGGCGCCGGGCGCTTCCGCCAAGACCTCTACT
ACCGCCTCAACGTCATCGAGCTGCGCGTACCGCCGCTGCGCGAACGCCGCGAGGACATCCCGCTGCTCGCCGAACGCATC
CTCAAGCGCCTGGCCGGCGACACCGGCCTGCCGGCCGCCAGACTGACCGGCGACGCACAGGAGAAGCTGAAGAACTACCG
CTTTCCGGGCAACGTCCGCGAGCTGGAAAACATGCTGGAGCGCGCCTACACCCTGTGCGAAGACGACCAGATCCAGCCTC
ACGACCTGCGCCTGGCCGATTCGCCGGGTGCCAGCCAGGAAGGCGCGGCGAGCCTGAGCGAAATCGACAACCTCGAGGAC
TACCTGGAAGACATCGAGCGGAAGCTGATCATGCAGGCACTCGAGGAGACGCGCTGGAACCGCACCGCCGCGGCCCAGCG
CCTGGGCCTGACGTTCCGCTCGATGCGCTACCGCCTGAAAAAGCTGGGCATCGACTGA

Domains

Predicted by InterproScan.

Response regulator receiver domain (Response_reg)

(7-115)

Sigma-54 interaction domain (Sigma54_activat)

(135-301)

Bacterial regulatory protein, Fis family (HTH_8)

(402-442)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilR Pseudomonas aeruginosa PAK

99.775

100

0.998
  pilR Acinetobacter baumannii strain A118

50.98

100

0.526
  luxO Vibrio cholerae strain A1552

37.946

100

0.382

Multiple sequence alignment