Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilS   Type   Regulator
Locus tag   ACFELC_RS24605 Genome accession   NZ_CP172849
Coordinates   5264752..5266344 (+) Length   530 a.a.
NCBI ID   WP_039028118.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain 238641E_ID020     
Function   regulate pilin expression (predicted from homology)   
Competence regulation

Genomic Context

Location: 5259752..5271344
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACFELC_RS24585 (ACFELC_24585) pgeF 5261502..5262230 (-) 729 WP_400564481.1 peptidoglycan editing factor PgeF -
  ACFELC_RS24590 (ACFELC_24590) rluD 5262227..5263189 (-) 963 WP_033972915.1 23S rRNA pseudouridine(1911/1915/1917) synthase RluD -
  ACFELC_RS24595 (ACFELC_24595) - 5263335..5264360 (+) 1026 WP_003102590.1 outer membrane protein assembly factor BamD -
  ACFELC_RS24600 (ACFELC_24600) - 5264517..5264762 (+) 246 WP_003102591.1 PP0621 family protein -
  ACFELC_RS24605 (ACFELC_24605) pilS 5264752..5266344 (+) 1593 WP_039028118.1 two-component system sensor histidine kinase PilS Regulator
  ACFELC_RS24610 (ACFELC_24610) pilR 5266359..5267696 (+) 1338 WP_400564483.1 two-component system response regulator PilR Regulator
  ACFELC_RS24615 (ACFELC_24615) thiO 5267742..5268836 (-) 1095 WP_033976474.1 glycine oxidase ThiO -
  ACFELC_RS24620 (ACFELC_24620) - 5268971..5269480 (+) 510 WP_033976381.1 Tfp pilus assembly protein FimT/FimU -
  ACFELC_RS24625 (ACFELC_24625) - 5269586..5270092 (+) 507 WP_003134935.1 Tfp pilus assembly protein FimT/FimU -
  ACFELC_RS24630 (ACFELC_24630) pilV 5270083..5270640 (+) 558 WP_003125211.1 type 4a pilus minor pilin PilV Machinery gene

Sequence

Protein


Download         Length: 530 a.a.        Molecular weight: 59116.28 Da        Isoelectric Point: 6.4439

>NTDB_id=1069182 ACFELC_RS24605 WP_039028118.1 5264752..5266344(+) (pilS) [Pseudomonas aeruginosa strain 238641E_ID020]
MRAERLRLSEEQGQRILRLYHLYRLTIGLVLVLLISSELEDQVLKLVHPELFHVGSWCYLVFNILVALFLPPSRQLLPIF
ILALTDVLMLCGLFYAGGGVPSGIGSLLVVAVAIANILLRGRIGLVIAAAASLGLLYLTFFLSLSSPDATNHYVQAGGLG
TLCFAAALVIQALVRRQEQTETLAEERAETVANLEELNALILQRMRTGILVVDSRQAILLANQAALGLLRQDDVQGASLG
RHSPMLMHCMKQWRLNPSLRPPTLKVLPDGPTVQPSFISLNREDDQHVLIFLEDISQIAQQAQQMKLAGLGRLTAGIAHE
IRNPLGAISHAAQLLQESEELDAPDRRLTQIIQDQSKRMNLVIENVLQLSRRRQAEPQQLDLKEWLQRFVDEYPGRLRND
SELHLQLGADDIQTRMDPHQLNQVLSNLVQNGLRYSAQAHGRGQVWLSLVRDPESDLPVLEVIDDGPGVPTDKLNNLFEP
FFTTESKGTGLGLYLSRELCESNQARIDYRNREEGGGCFRITFAHPRKLS

Nucleotide


Download         Length: 1593 bp        

>NTDB_id=1069182 ACFELC_RS24605 WP_039028118.1 5264752..5266344(+) (pilS) [Pseudomonas aeruginosa strain 238641E_ID020]
GTGCGCGCTGAACGGCTACGGCTGAGCGAGGAGCAGGGGCAACGCATCCTCCGTCTGTACCACCTGTACCGCCTGACCAT
CGGCCTGGTACTGGTCCTGCTGATCTCCAGCGAACTGGAAGATCAGGTCCTCAAGCTCGTCCACCCTGAACTGTTCCATG
TCGGCAGTTGGTGCTACCTGGTCTTCAACATCCTGGTCGCGCTGTTCCTGCCGCCGTCGCGGCAATTGCTGCCGATCTTC
ATCCTCGCGCTCACCGACGTACTGATGCTTTGCGGCCTGTTCTACGCAGGTGGCGGCGTACCCAGCGGCATCGGCAGCCT
GCTGGTGGTGGCGGTGGCCATTGCCAACATCCTGCTGCGCGGGCGCATCGGCCTGGTCATCGCGGCAGCGGCCAGCCTCG
GCCTGCTCTACCTGACCTTCTTCCTCAGCCTGAGCAGTCCGGACGCCACCAACCACTACGTCCAGGCCGGCGGCCTCGGC
ACCCTGTGCTTCGCCGCCGCGCTGGTGATCCAGGCCCTGGTGCGGCGCCAGGAGCAGACCGAAACGCTGGCCGAAGAACG
CGCCGAGACGGTCGCCAACCTGGAGGAACTCAACGCATTGATCCTGCAGCGTATGCGCACCGGCATCCTTGTGGTCGATA
GCCGTCAGGCCATCCTCCTCGCCAACCAGGCCGCCCTCGGCCTGCTCAGGCAGGACGACGTACAGGGCGCCAGCCTCGGC
CGCCACAGCCCGATGCTGATGCACTGCATGAAGCAATGGCGCCTGAATCCCAGCCTCCGTCCACCGACGCTCAAGGTGCT
GCCGGATGGCCCGACGGTGCAACCCAGCTTTATCAGCCTCAACCGCGAAGACGACCAGCACGTGCTGATCTTCCTCGAAG
ACATTTCGCAGATCGCCCAGCAGGCGCAGCAGATGAAGCTGGCCGGTCTCGGCCGCCTGACCGCCGGCATCGCCCATGAG
ATCCGCAACCCGCTGGGCGCGATCAGCCACGCCGCCCAACTGCTGCAGGAGTCCGAGGAGCTGGACGCCCCGGACCGGCG
CCTGACGCAGATCATCCAGGACCAGTCGAAGCGGATGAACCTGGTCATCGAGAACGTCCTGCAGCTCTCCCGTCGCCGCC
AGGCCGAACCGCAGCAGCTCGACCTGAAGGAGTGGCTTCAGCGGTTCGTCGACGAATACCCCGGCAGGCTGCGCAACGAT
AGCGAACTGCACCTGCAACTCGGTGCCGACGACATCCAGACCCGCATGGACCCACACCAATTGAACCAGGTGCTGAGCAA
CCTGGTGCAGAACGGTCTTCGCTACAGCGCCCAGGCGCACGGGCGCGGCCAGGTCTGGCTGAGCCTCGTGCGCGACCCGG
AGAGCGACCTGCCGGTGCTGGAAGTCATCGACGACGGTCCCGGCGTACCGACGGACAAACTGAACAACCTGTTCGAACCC
TTCTTTACTACAGAAAGCAAAGGCACCGGCCTGGGCCTCTATCTCTCCCGCGAGCTCTGCGAGAGCAACCAGGCACGGAT
CGACTACCGCAATCGCGAGGAAGGCGGCGGCTGCTTCCGCATCACCTTCGCCCACCCGCGCAAACTCAGCTGA

Domains

Predicted by InterproScan.

His Kinase A (phospho-acceptor) domain (HisKA)

(310-373)

Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (HATPase_c)

(417-523)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilS Pseudomonas aeruginosa PAK

99.057

100

0.991

Multiple sequence alignment