Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   ACG1BZ_RS01275 Genome accession   NZ_CP172449
Coordinates   272697..273773 (+) Length   358 a.a.
NCBI ID   WP_413664078.1    Uniprot ID   -
Organism   Microbulbifer sp. CNSA002     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 267697..278773
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACG1BZ_RS01265 (ACG1BZ_01265) - 269342..269821 (+) 480 WP_413664076.1 chemotaxis protein CheW -
  ACG1BZ_RS01270 (ACG1BZ_01270) - 269888..272548 (-) 2661 WP_413664077.1 penicillin-binding protein 1A -
  ACG1BZ_RS01275 (ACG1BZ_01275) pilM 272697..273773 (+) 1077 WP_413664078.1 pilus assembly protein PilM Machinery gene
  ACG1BZ_RS01280 (ACG1BZ_01280) - 273775..274350 (+) 576 WP_413664079.1 PilN domain-containing protein -
  ACG1BZ_RS01285 (ACG1BZ_01285) - 274362..274979 (+) 618 WP_413664080.1 type 4a pilus biogenesis protein PilO -
  ACG1BZ_RS01290 (ACG1BZ_01290) - 274976..275506 (+) 531 WP_413664081.1 pilus assembly protein PilP -
  ACG1BZ_RS01295 (ACG1BZ_01295) pilQ 275506..277683 (+) 2178 WP_413664082.1 type IV pilus secretin PilQ -
  ACG1BZ_RS01300 (ACG1BZ_01300) aroK 277683..278204 (+) 522 WP_413665828.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 358 a.a.        Molecular weight: 38985.32 Da        Isoelectric Point: 4.3797

>NTDB_id=1067888 ACG1BZ_RS01275 WP_413664078.1 272697..273773(+) (pilM) [Microbulbifer sp. CNSA002]
MGIFPFLSKGPKAMLGLDISSTAVKLLELSRNGDKYRVESYAVEPLPPNAVVDKNINDVGATAEAIRKVVRRSKTRLREA
AVAVSGSAVITKTLEMPADLTEEALEAQIAIEADQYIPYPLEEVSLDFEVQGPSEKGEGQMEVLLAACRTENIEQRISAL
GEAELEPGVVDVEAYAIERTYTLLEDQLPPQEQLVVAVIDIGATMSALSVLVDGKTVYTREQLFGGRQLTDEIQRRYGLS
VEEATLAKRQGGSGLPDDYFAEVLEPFRDAVVQQVTRSLQFFYSSTSYSDVDHILLGGGVAAMEGIGELVSEKLGRPTLI
ANPFHKMSVSSRVNRQALANEAPSLMIATGLAMREKDY

Nucleotide


Download         Length: 1077 bp        

>NTDB_id=1067888 ACG1BZ_RS01275 WP_413664078.1 272697..273773(+) (pilM) [Microbulbifer sp. CNSA002]
ATGGGAATTTTCCCTTTTCTGAGCAAAGGCCCCAAGGCCATGTTGGGACTCGATATTAGCTCTACTGCAGTCAAGTTGCT
GGAGCTAAGTCGCAACGGCGACAAATATCGTGTCGAAAGTTATGCCGTGGAACCTCTACCACCCAATGCCGTAGTTGATA
AGAATATCAACGATGTGGGGGCGACGGCTGAGGCTATCCGCAAAGTTGTACGACGCTCCAAGACGCGCTTACGCGAAGCT
GCCGTTGCTGTGTCTGGATCAGCGGTGATCACCAAGACCCTCGAAATGCCGGCGGACCTCACTGAGGAAGCATTGGAAGC
CCAGATCGCAATCGAGGCTGACCAGTATATTCCTTATCCGCTTGAAGAAGTCAGCCTGGACTTTGAGGTGCAGGGGCCTT
CCGAGAAGGGGGAAGGTCAAATGGAGGTCCTGCTTGCGGCTTGCCGCACAGAAAACATTGAACAGCGGATCTCTGCGTTG
GGTGAGGCCGAACTGGAACCCGGTGTGGTGGATGTTGAGGCTTACGCAATAGAGCGAACTTACACATTGCTGGAAGATCA
GCTCCCTCCTCAAGAGCAACTAGTCGTAGCCGTTATTGATATCGGAGCGACGATGAGTGCCCTGTCAGTTCTTGTGGATG
GCAAAACAGTGTATACCCGCGAACAACTCTTTGGGGGGCGTCAGCTCACCGACGAGATACAGCGTCGCTATGGGCTCTCA
GTAGAAGAAGCTACTCTGGCGAAACGCCAGGGAGGTAGTGGCTTACCAGATGATTATTTTGCTGAAGTGTTGGAGCCTTT
CAGAGACGCGGTAGTTCAACAGGTAACCCGTTCTTTACAATTCTTTTATTCCTCTACCTCATATAGTGATGTGGACCATA
TCCTTCTCGGCGGTGGAGTTGCGGCGATGGAAGGAATTGGTGAACTTGTTAGTGAAAAGCTGGGTCGACCGACATTGATT
GCTAATCCATTTCACAAAATGAGTGTGTCTTCACGGGTTAATCGCCAGGCTTTGGCAAATGAAGCCCCCTCACTAATGAT
TGCCACAGGGTTGGCGATGAGGGAGAAGGACTACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(16-355)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

51.862

97.486

0.506
  comM Acinetobacter nosocomialis M2

51.862

97.486

0.506
  comM Acinetobacter baylyi ADP1

49.133

96.648

0.475
  pilM Legionella pneumophila strain ERS1305867

43.503

98.883

0.43

Multiple sequence alignment