Detailed information NTG1067781

Bioinformatically predicted

Overview

• Name: comGF
• Type: Machinery gene
• Locus tag: ACH5DO_RS13380
• Genome accession: NZ_CP172432
• Coordinates: 2740536..2741033 (+)
• Length: 165 a.a.
• NCBI ID: WP_001825340.1
• Uniprot ID: A0A2S6DDC2
• Organism: Staphylococcus aureus strain DG29
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2735536..2746033

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACH5DO_RS13340 (ACH5DO_13340)	-	2735580..2736566 (+)	987	WP_000161315.1	ROK family glucokinase	-
ACH5DO_RS13345 (ACH5DO_13345)	-	2736566..2736895 (+)	330	WP_001018871.1	MTH1187 family thiamine-binding protein	-
ACH5DO_RS13350 (ACH5DO_13350)	-	2736892..2737515 (+)	624	WP_001223008.1	MBL fold metallo-hydrolase	-
ACH5DO_RS13355 (ACH5DO_13355)	comGA	2737567..2738541 (+)	975	WP_000697220.1	competence type IV pilus ATPase ComGA	Machinery gene
ACH5DO_RS13360 (ACH5DO_13360)	comGB	2738513..2739583 (+)	1071	WP_000776425.1	competence type IV pilus assembly protein ComGB	Machinery gene
ACH5DO_RS13365 (ACH5DO_13365)	comGC	2739597..2739908 (+)	312	WP_000472256.1	competence type IV pilus major pilin ComGC	Machinery gene
ACH5DO_RS13370 (ACH5DO_13370)	comGD	2739886..2740332 (+)	447	WP_001796473.1	competence type IV pilus minor pilin ComGD	Machinery gene
ACH5DO_RS13375 (ACH5DO_13375)	comGE	2740319..2740618 (+)	300	WP_000844413.1	hypothetical protein	Machinery gene
ACH5DO_RS13380 (ACH5DO_13380)	comGF	2740536..2741033 (+)	498	WP_001825340.1	competence type IV pilus minor pilin ComGF	Machinery gene
ACH5DO_RS13385 (ACH5DO_13385)	-	2741130..2741276 (+)	147	WP_001789879.1	hypothetical protein	-
ACH5DO_RS13390 (ACH5DO_13390)	-	2741266..2741790 (+)	525	WP_001015120.1	shikimate kinase	-
ACH5DO_RS13395 (ACH5DO_13395)	gcvT	2741949..2743040 (+)	1092	WP_000093349.1	glycine cleavage system aminomethyltransferase GcvT	-
ACH5DO_RS13400 (ACH5DO_13400)	gcvPA	2743060..2744406 (+)	1347	WP_000019698.1	aminomethyl-transferring glycine dehydrogenase subunit GcvPA	-
ACH5DO_RS13405 (ACH5DO_13405)	gcvPB	2744399..2745871 (+)	1473	WP_000202179.1	aminomethyl-transferring glycine dehydrogenase subunit GcvPB	-

Sequence

Protein

• Length: 165 a.a.
• Molecular weight: 19179.92 Da
• Isoelectric Point: 10.2192

>NTDB_id=1067781 ACH5DO_RS13380 WP_001825340.1 2740536..2741033(+) (comGF) [Staphylococcus aureus strain DG29]
MILSKVTNKFVLFQKIPLLIKRHVYSINVKAFSLIEMLVAMMVISITLLIVPDLIRLSKTFLIESRELTTVDFEFFSRDI
LEDFKGVDRNDIEIRQQRIILHKGEEMIEYKLINNKIIKVVNDRGNITLINNVTAFTANIYYKSIIKITITVKVGTNLQT
KTIYV

Nucleotide

• Length: 498 bp

>NTDB_id=1067781 ACH5DO_RS13380 WP_001825340.1 2740536..2741033(+) (comGF) [Staphylococcus aureus strain DG29]
ATGATATTAAGCAAAGTGACCAACAAATTTGTGCTATTTCAAAAAATACCACTTCTTATCAAAAGACATGTATACAGTAT
TAATGTCAAAGCTTTTTCGCTTATTGAAATGTTAGTAGCGATGATGGTTATAAGCATAACTTTACTAATTGTTCCAGACT
TAATTAGACTTAGCAAAACTTTTCTAATTGAAAGTAGGGAATTAACAACTGTAGATTTCGAATTTTTCTCAAGAGATATT
CTAGAGGATTTTAAAGGTGTAGATAGAAACGATATTGAAATTAGGCAACAGCGTATCATTTTACATAAAGGTGAAGAAAT
GATCGAATACAAATTAATAAATAATAAAATTATTAAAGTTGTAAATGACAGAGGAAATATAACATTGATTAATAATGTTA
CAGCATTTACTGCAAATATCTACTATAAATCTATTATTAAAATAACGATAACAGTTAAAGTCGGTACAAATCTGCAGACT
AAAACTATTTATGTATAA

Domains

Predicted by InterproScan.

• Prokaryotic N-terminal methylation motif (N_methyl) (28-51)

• Putative Competence protein ComGF (ComGF) (68-155)

3D structure

• AlphaFold DB: A0A2S6DDC2

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comGF	Staphylococcus aureus N315	98.182	100	0.982
comGF	Staphylococcus aureus MW2	96.97	100	0.97