Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB   Type   Machinery gene
Locus tag   ACH5DO_RS13360 Genome accession   NZ_CP172432
Coordinates   2738513..2739583 (+) Length   356 a.a.
NCBI ID   WP_000776425.1    Uniprot ID   -
Organism   Staphylococcus aureus strain DG29     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2733513..2744583
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACH5DO_RS13330 (ACH5DO_13330) - 2733936..2735399 (+) 1464 WP_001017856.1 rhomboid family intramembrane serine protease -
  ACH5DO_RS13335 (ACH5DO_13335) - 2735380..2735583 (+) 204 WP_000087559.1 YqgQ family protein -
  ACH5DO_RS13340 (ACH5DO_13340) - 2735580..2736566 (+) 987 WP_000161315.1 ROK family glucokinase -
  ACH5DO_RS13345 (ACH5DO_13345) - 2736566..2736895 (+) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  ACH5DO_RS13350 (ACH5DO_13350) - 2736892..2737515 (+) 624 WP_001223008.1 MBL fold metallo-hydrolase -
  ACH5DO_RS13355 (ACH5DO_13355) comGA 2737567..2738541 (+) 975 WP_000697220.1 competence type IV pilus ATPase ComGA Machinery gene
  ACH5DO_RS13360 (ACH5DO_13360) comGB 2738513..2739583 (+) 1071 WP_000776425.1 competence type IV pilus assembly protein ComGB Machinery gene
  ACH5DO_RS13365 (ACH5DO_13365) comGC 2739597..2739908 (+) 312 WP_000472256.1 competence type IV pilus major pilin ComGC Machinery gene
  ACH5DO_RS13370 (ACH5DO_13370) comGD 2739886..2740332 (+) 447 WP_001796473.1 competence type IV pilus minor pilin ComGD Machinery gene
  ACH5DO_RS13375 (ACH5DO_13375) comGE 2740319..2740618 (+) 300 WP_000844413.1 hypothetical protein Machinery gene
  ACH5DO_RS13380 (ACH5DO_13380) comGF 2740536..2741033 (+) 498 WP_001825340.1 competence type IV pilus minor pilin ComGF Machinery gene
  ACH5DO_RS13385 (ACH5DO_13385) - 2741130..2741276 (+) 147 WP_001789879.1 hypothetical protein -
  ACH5DO_RS13390 (ACH5DO_13390) - 2741266..2741790 (+) 525 WP_001015120.1 shikimate kinase -
  ACH5DO_RS13395 (ACH5DO_13395) gcvT 2741949..2743040 (+) 1092 WP_000093349.1 glycine cleavage system aminomethyltransferase GcvT -
  ACH5DO_RS13400 (ACH5DO_13400) gcvPA 2743060..2744406 (+) 1347 WP_000019698.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPA -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 41489.97 Da        Isoelectric Point: 10.1030

>NTDB_id=1067777 ACH5DO_RS13360 WP_000776425.1 2738513..2739583(+) (comGB) [Staphylococcus aureus strain DG29]
MKLQWINTFKLHSKKRQLSKVQQIDLLSNLCNLLKYGFTLYQSFQFLNLQMTYKNKQLGTTILSEISNGAPCNQILSLIG
YSDTIVMQVYLAERFGNIIDVLEETVNYMKVNRKSEQRLLKTLQYPLILVSIFIAMIIILNLTVIPQFQQLYTSMNIQLS
SFQKTLSFFITSLPTIIVVMLIIVSMLAIIMKLIYNKLNMLNKINFVMKLPLISGYFQLFKTYFVTNELVLFYKNGITLQ
SIVDVYINHSSDPFRQFLGKYLLTYSEMGYGLPQILEKLKCFKPQLIKFVLQGEKRGKLEVELKLYSQILVKQIEDKAIK
QTQFLQPILFLILGLFIVAIYLVIMLPMFQMMQSIK

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=1067777 ACH5DO_RS13360 WP_000776425.1 2738513..2739583(+) (comGB) [Staphylococcus aureus strain DG29]
GTGAAACTACAATGGATAAATACATTTAAACTACATTCTAAGAAGCGACAATTAAGTAAGGTCCAACAAATCGATTTACT
TTCAAATTTATGTAATTTGTTGAAATATGGTTTCACTCTGTATCAAAGTTTTCAATTTTTAAATCTTCAAATGACATATA
AAAATAAGCAATTAGGCACCACCATTCTAAGTGAAATTTCAAATGGTGCACCATGCAACCAGATATTATCACTGATAGGT
TATAGCGATACTATCGTCATGCAAGTATATTTGGCAGAAAGATTTGGCAATATCATAGACGTTCTAGAAGAAACCGTAAA
TTATATGAAAGTGAATAGAAAGTCAGAACAACGATTGTTAAAGACACTGCAATACCCCTTAATACTAGTCTCTATTTTTA
TTGCTATGATTATTATATTAAACCTCACAGTAATTCCACAGTTTCAACAATTATATACTTCTATGAATATTCAACTATCT
TCTTTTCAAAAAACATTGTCTTTTTTCATTACCAGCTTACCTACTATAATTGTAGTAATGCTCATAATAGTATCTATGTT
GGCTATTATTATGAAATTAATTTATAACAAATTAAATATGCTCAATAAGATAAACTTTGTGATGAAACTACCGCTAATAT
CGGGTTATTTCCAATTATTTAAAACTTATTTTGTAACTAATGAATTAGTGTTGTTTTATAAAAATGGTATTACACTTCAA
TCAATAGTAGACGTTTATATTAACCATAGTAGTGATCCATTTAGACAGTTTCTAGGTAAATACTTATTAACTTATTCAGA
AATGGGATATGGTTTACCTCAAATTTTAGAAAAACTAAAATGCTTTAAGCCTCAATTAATTAAGTTTGTGCTACAAGGTG
AAAAGAGAGGAAAGCTAGAAGTAGAACTAAAGTTATATTCGCAAATATTAGTAAAACAAATAGAAGATAAAGCGATAAAA
CAGACTCAGTTTTTACAGCCTATTTTATTTTTGATTTTAGGTTTATTTATTGTCGCAATTTATTTAGTAATTATGTTACC
AATGTTTCAAATGATGCAAAGTATAAAATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(227-347)

Type II secretion system (T2SS), protein F (T2SSF)

(27-146)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB Staphylococcus aureus MW2

99.438

100

0.994
  comGB Staphylococcus aureus N315

99.438

100

0.994

Multiple sequence alignment