Detailed information    

insolico Bioinformatically predicted

Overview

Name   ruvB   Type   Machinery gene
Locus tag   EN18_RS06485 Genome accession   NZ_CP007634
Coordinates   1454130..1455134 (-) Length   334 a.a.
NCBI ID   WP_000568491.1    Uniprot ID   Q9KR02
Organism   Vibrio cholerae strain 2012EL-2176     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1449130..1460134
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EN18_RS06455 (EN18_12325) tolQ 1449252..1449935 (-) 684 WP_000123084.1 protein TolQ -
  EN18_RS06460 (EN18_12330) ybgC 1449925..1450332 (-) 408 WP_001091290.1 tol-pal system-associated acyl-CoA thioesterase -
  EN18_RS06465 (EN18_12335) ybgE 1450501..1450818 (-) 318 WP_001286617.1 cyd operon protein YbgE -
  EN18_RS06470 (EN18_12340) cydX 1450811..1450918 (-) 108 WP_000270285.1 cytochrome bd-I oxidase subunit CydX -
  EN18_RS06475 (EN18_12345) cydB 1450931..1452067 (-) 1137 WP_000460767.1 cytochrome d ubiquinol oxidase subunit II -
  EN18_RS06480 (EN18_12350) cydA 1452085..1453671 (-) 1587 WP_000568056.1 cytochrome ubiquinol oxidase subunit I -
  EN18_RS06485 (EN18_12355) ruvB 1454130..1455134 (-) 1005 WP_000568491.1 Holliday junction branch migration DNA helicase RuvB Machinery gene
  EN18_RS06490 (EN18_12360) ruvA 1455190..1455804 (-) 615 WP_000580359.1 Holliday junction branch migration protein RuvA Machinery gene
  EN18_RS06495 (EN18_12365) ruvC 1455901..1456422 (-) 522 WP_000111361.1 crossover junction endodeoxyribonuclease RuvC -
  EN18_RS06500 (EN18_12370) cysS 1456510..1457889 (-) 1380 WP_000913180.1 cysteine--tRNA ligase -
  EN18_RS06505 (EN18_12375) - 1458035..1458529 (+) 495 WP_000631302.1 peptidylprolyl isomerase -
  EN18_RS06510 (EN18_12380) lpxH 1458570..1459310 (+) 741 WP_000557000.1 UDP-2,3-diacylglucosamine diphosphatase -

Sequence

Protein


Download         Length: 334 a.a.        Molecular weight: 37298.75 Da        Isoelectric Point: 5.0460

>NTDB_id=106776 EN18_RS06485 WP_000568491.1 1454130..1455134(-) (ruvB) [Vibrio cholerae strain 2012EL-2176]
MIEADRLIAPISNHFKDEEVIDRAIRPKKLADYQGQDHVRDQMEIFIQAAQMRQEALDHLLIFGPPGLGKTTLANIVANE
MGVNIRTTSGPVLEKAGDLAALLTNLEENDVLFIDEIHRLSPMVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLI
GATTRAGSLTSPLRDRFGIVQRLEYYKVADLQHIVQRSAQCLGLSMDSEGALEVARRARGTPRIANRLLRRVRDYAEVKG
DGHICAQTADRALNMLDVDHQGFDYMDRKLLLAIMEKFSGGPVGIDNLAAAIGEEKDTIEDVLEPFLIQQGYLQRTPRGR
IATDRAYLHFGIEK

Nucleotide


Download         Length: 1005 bp        

>NTDB_id=106776 EN18_RS06485 WP_000568491.1 1454130..1455134(-) (ruvB) [Vibrio cholerae strain 2012EL-2176]
ATGATAGAAGCCGATCGTTTAATTGCTCCAATCAGCAACCACTTCAAAGATGAGGAAGTGATTGATCGAGCGATTCGTCC
CAAAAAGCTTGCCGATTACCAAGGTCAAGATCATGTTCGTGATCAGATGGAAATCTTCATTCAAGCGGCACAAATGCGCC
AAGAAGCCTTAGATCATCTGCTGATTTTCGGGCCTCCGGGCTTGGGTAAAACCACCTTGGCCAACATAGTCGCTAACGAA
ATGGGCGTGAATATTCGTACTACTTCAGGTCCTGTATTGGAAAAAGCGGGAGATTTAGCGGCACTGCTAACCAATCTTGA
AGAAAACGATGTGCTGTTTATTGATGAAATCCATCGTCTGAGCCCAATGGTTGAGGAAGTACTGTATCCCGCTATGGAAG
ATTACCAACTCGATATCATGATTGGTGAAGGCCCAGCTGCACGCTCAATCAAAATCGATCTCCCTCCTTTTACTTTAATT
GGAGCCACCACACGTGCAGGCTCGCTGACTTCACCACTGCGTGACCGATTTGGGATTGTGCAACGATTGGAGTATTACAA
AGTCGCCGATTTACAACACATAGTGCAACGTAGTGCACAGTGTCTCGGTCTTTCGATGGATAGTGAGGGCGCTCTTGAAG
TGGCTCGCCGTGCACGTGGAACCCCACGGATTGCCAATCGCCTGCTGCGCCGGGTACGAGATTATGCCGAAGTAAAAGGG
GATGGGCACATTTGTGCGCAAACCGCTGACAGGGCACTCAATATGCTTGATGTTGACCATCAAGGCTTTGATTACATGGA
TAGAAAGCTGCTGTTAGCAATAATGGAAAAGTTTTCAGGTGGCCCAGTCGGAATCGATAACCTTGCGGCAGCTATTGGCG
AAGAGAAAGATACGATAGAAGATGTTCTTGAGCCATTTCTCATTCAGCAGGGTTATTTACAACGTACACCACGCGGTCGT
ATCGCGACAGATCGCGCTTATTTGCATTTCGGTATTGAAAAATAA

Domains

Predicted by InterProScan.

Holliday junction DNA helicase RuvB P-loop domain (RuvB_N)

(25-183)

RuvB C-terminal winged helix domain (WHD_RuvB)

(261-330)

RuvB AAA lid domain (AAA_lid_4)

(186-259)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q9KR02

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ruvB Bacillus subtilis subsp. subtilis str. 168

61.682

96.108

0.593
  ruvB Streptococcus pneumoniae TIGR4

59.937

94.91

0.569
  ruvB Streptococcus pneumoniae R6

59.937

94.91

0.569
  ruvB Streptococcus pneumoniae D39

59.937

94.91

0.569
  ruvB Synechocystis sp. PCC 6803

55.663

92.515

0.515
  ruvB Helicobacter pylori 26695

54.276

91.018

0.494

Multiple sequence alignment