Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   ACH45O_RS02355 Genome accession   NZ_CP172430
Coordinates   514589..515641 (+) Length   350 a.a.
NCBI ID   WP_028683780.1    Uniprot ID   A0AAJ0ZMY7
Organism   Pseudomonas chlororaphis strain ZL3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 509589..520641
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACH45O_RS02345 (ACH45O_02345) - 510541..511809 (+) 1269 WP_007925851.1 malic enzyme-like NAD(P)-binding protein -
  ACH45O_RS02350 (ACH45O_02350) - 511937..514372 (-) 2436 WP_397446173.1 penicillin-binding protein 1A -
  ACH45O_RS02355 (ACH45O_02355) pilM 514589..515641 (+) 1053 WP_028683780.1 pilus assembly protein PilM Machinery gene
  ACH45O_RS02360 (ACH45O_02360) - 515641..516207 (+) 567 WP_397443266.1 PilN domain-containing protein -
  ACH45O_RS02365 (ACH45O_02365) - 516204..516821 (+) 618 WP_096372866.1 type 4a pilus biogenesis protein PilO -
  ACH45O_RS02370 (ACH45O_02370) - 516818..517345 (+) 528 WP_038582723.1 pilus assembly protein PilP -
  ACH45O_RS02375 (ACH45O_02375) pilQ 517359..519431 (+) 2073 WP_397443268.1 type IV pilus secretin PilQ Machinery gene
  ACH45O_RS02380 (ACH45O_02380) aroK 519436..519954 (+) 519 WP_009041824.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 350 a.a.        Molecular weight: 37047.51 Da        Isoelectric Point: 5.0008

>NTDB_id=1067713 ACH45O_RS02355 WP_028683780.1 514589..515641(+) (pilM) [Pseudomonas chlororaphis strain ZL3]
MLGLFSKKASSLLGIDISSTSVKLLELSRVGGRFRVEAYGVEPLPANAVVEKNIVEPEGVGHALSRLLARARPGAKHAAL
AMAGSAVISKVIEMEAGLCDDDRESQLKVDADQYIPYPLDEAAIDFDVLGYSARNPERVDVLLAACRKEHIEVREAALEL
AGLTARVVDLEVCALERSFALAAGASVARTVALVDIGATLTTLSVVHEGQIIYSREQLFGGHQLTEHIQRRYGLSKEEAG
LAKKQGGLPDDYASEVLQPFQEAVVQQVSRSLQFFQASGPQRQVDSILLAGGSASVPGLAPLIEQRLGLPVLIANPFAGM
SIGGKVDAAALASEAPSLMIACGLALRSFD

Nucleotide


Download         Length: 1053 bp        

>NTDB_id=1067713 ACH45O_RS02355 WP_028683780.1 514589..515641(+) (pilM) [Pseudomonas chlororaphis strain ZL3]
GTGCTAGGACTCTTCAGTAAAAAGGCGAGTTCCCTTCTGGGGATCGATATCAGCTCCACCTCGGTCAAGCTTCTTGAGTT
GAGCCGTGTCGGCGGCCGTTTTCGGGTCGAGGCCTATGGGGTGGAACCGTTGCCGGCCAACGCCGTGGTCGAAAAAAACA
TCGTCGAGCCCGAAGGGGTGGGGCATGCCTTGTCTCGCCTGCTGGCCAGGGCCCGGCCGGGAGCAAAGCACGCGGCGCTG
GCGATGGCCGGCTCGGCGGTGATCAGCAAGGTCATCGAGATGGAGGCCGGGCTTTGCGATGACGACCGGGAGAGCCAGCT
CAAGGTCGACGCCGACCAGTACATCCCCTATCCGCTGGATGAGGCCGCCATCGATTTCGACGTCCTCGGCTATTCGGCGC
GCAATCCCGAACGCGTCGATGTGCTGTTGGCGGCCTGCCGCAAGGAACACATCGAAGTTCGCGAGGCGGCGCTGGAGCTC
GCCGGGCTGACGGCGCGGGTGGTGGACCTGGAAGTCTGCGCGCTGGAGCGCTCGTTCGCCCTGGCGGCCGGGGCGTCGGT
TGCCCGGACTGTGGCGCTGGTCGATATCGGAGCCACCCTGACCACCCTGAGCGTTGTGCATGAAGGGCAAATCATCTATT
CCCGCGAGCAGTTGTTTGGTGGGCACCAACTGACCGAGCACATCCAGCGTCGTTACGGGCTGTCGAAAGAAGAGGCCGGC
CTGGCAAAAAAACAGGGCGGCCTGCCGGACGATTACGCCAGTGAGGTGCTGCAACCCTTTCAGGAGGCGGTCGTGCAACA
GGTTTCGCGCTCGTTGCAGTTTTTCCAGGCCTCCGGCCCGCAGCGCCAGGTCGACTCCATCCTGTTGGCGGGCGGCAGCG
CCTCGGTTCCGGGGCTTGCGCCGTTGATCGAGCAGCGCCTGGGCTTGCCTGTGCTGATCGCCAACCCCTTCGCTGGCATG
AGCATCGGCGGCAAGGTCGACGCCGCGGCCCTGGCCAGCGAGGCTCCATCCTTGATGATCGCCTGTGGGCTGGCCTTGCG
GAGTTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-349)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

55.398

100

0.557
  comM Acinetobacter nosocomialis M2

55.114

100

0.554
  comM Acinetobacter baylyi ADP1

52.557

100

0.529
  pilM Legionella pneumophila strain ERS1305867

45.48

100

0.46

Multiple sequence alignment