Detailed information    

insolico Bioinformatically predicted

Overview

Name   ruvB   Type   Machinery gene
Locus tag   EN73_RS00420 Genome accession   NZ_CP007632
Coordinates   65526..66524 (+) Length   332 a.a.
NCBI ID   WP_000196633.1    Uniprot ID   Q3K3X8
Organism   Streptococcus agalactiae strain NGBS572     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 60526..71524
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EN73_RS00400 (EN73_00400) - 61072..61329 (+) 258 WP_000590468.1 hypothetical protein -
  EN73_RS00405 (EN73_00405) - 61695..62798 (+) 1104 WP_001867169.1 DUF6287 domain-containing protein -
  EN73_RS00410 (EN73_00410) purB 62884..64182 (+) 1299 WP_000893937.1 adenylosuccinate lyase -
  EN73_RS00415 (EN73_00415) comR 64335..65237 (+) 903 WP_000912098.1 helix-turn-helix domain-containing protein Regulator
  EN73_RS00420 (EN73_00420) ruvB 65526..66524 (+) 999 WP_000196633.1 Holliday junction branch migration DNA helicase RuvB Machinery gene
  EN73_RS00425 (EN73_00425) - 66676..67113 (+) 438 WP_000754817.1 low molecular weight protein-tyrosine-phosphatase -
  EN73_RS00430 (EN73_00430) - 67120..67500 (+) 381 WP_000787702.1 membrane protein -
  EN73_RS00435 (EN73_00435) - 67497..69275 (+) 1779 WP_001220911.1 acyltransferase family protein -

Sequence

Protein


Download         Length: 332 a.a.        Molecular weight: 37573.90 Da        Isoelectric Point: 4.5053

>NTDB_id=106644 EN73_RS00420 WP_000196633.1 65526..66524(+) (ruvB) [Streptococcus agalactiae strain NGBS572]
MTRFLDSDAMGDEELVERTLRPQYLREYIGQDKVKDQLKIFIEAAKLRDESLDHVLLFGPPGLGKTTMAFVIANELGVNL
KQTSGPAIEKSGDLVAILNDLEPGDVLFIDEIHRMPMAVEEVLYSAMEDFYIDIMIGAGETSRSVHLDLPPFTLIGATTR
AGMLSNPLRARFGITGHMEYYEENDLTEIIERTADIFEMKITYEAASELARRSRGTPRIANRLLKRVRDYAQIMGDGLID
DNITDKALTMLDVDHEGLDYVDQKILRTMIEMYNGGPVGLGTLSVNIAEERDTVEDMYEPYLIQKGFIMRTRTGRVATDK
AYEHLGYQRFDK

Nucleotide


Download         Length: 999 bp        

>NTDB_id=106644 EN73_RS00420 WP_000196633.1 65526..66524(+) (ruvB) [Streptococcus agalactiae strain NGBS572]
ATGACAAGATTTTTAGATAGTGATGCAATGGGTGACGAAGAATTGGTAGAACGTACACTTCGTCCGCAGTATTTAAGAGA
GTATATTGGACAAGATAAGGTTAAAGATCAGCTAAAAATATTTATTGAAGCTGCTAAATTGCGTGATGAGTCATTGGATC
ATGTGTTATTATTTGGCCCTCCTGGTTTAGGGAAAACAACCATGGCATTTGTAATTGCTAATGAGTTGGGTGTCAATCTC
AAACAAACATCAGGTCCCGCAATTGAAAAATCAGGGGATTTAGTAGCCATTTTAAATGATTTAGAACCAGGTGATGTTCT
TTTTATTGATGAAATTCATCGTATGCCGATGGCGGTTGAAGAGGTGCTCTATAGTGCAATGGAAGACTTTTATATTGACA
TTATGATTGGTGCAGGAGAAACTAGTAGAAGTGTTCATCTAGATTTGCCGCCCTTTACCTTAATTGGTGCAACGACACGT
GCAGGTATGTTATCTAATCCCTTACGTGCTCGCTTTGGTATTACAGGGCATATGGAGTATTATGAAGAAAATGATCTGAC
AGAAATTATTGAGCGTACAGCAGACATTTTTGAAATGAAAATTACTTATGAAGCTGCTTCTGAATTAGCGCGTCGCAGTC
GTGGAACGCCACGTATCGCTAACCGTTTATTGAAACGTGTTCGAGATTATGCTCAAATCATGGGAGATGGTTTGATAGAT
GACAATATTACAGATAAAGCATTAACGATGTTAGATGTTGATCACGAGGGGCTTGATTACGTCGATCAAAAAATCTTAAG
AACCATGATTGAAATGTATAATGGAGGTCCTGTTGGTTTAGGAACTCTATCCGTTAATATTGCTGAAGAACGAGATACTG
TTGAAGACATGTACGAACCTTATTTAATTCAAAAAGGTTTTATTATGCGTACCCGTACCGGTCGTGTAGCTACGGATAAG
GCATATGAACATTTAGGTTATCAGCGATTTGATAAATAA

Domains

Predicted by InterProScan.

Holliday junction DNA helicase RuvB P-loop domain (RuvB_N)

(20-178)

RuvB C-terminal winged helix domain (WHD_RuvB)

(256-325)

RuvB AAA lid domain (AAA_lid_4)

(181-254)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q3K3X8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ruvB Streptococcus pneumoniae TIGR4

90.06

100

0.901
  ruvB Streptococcus pneumoniae R6

89.759

100

0.898
  ruvB Streptococcus pneumoniae D39

89.759

100

0.898
  ruvB Bacillus subtilis subsp. subtilis str. 168

59.819

99.699

0.596
  ruvB Helicobacter pylori 26695

53.074

93.072

0.494
  ruvB Synechocystis sp. PCC 6803

50.479

94.277

0.476

Multiple sequence alignment