Detailed information NTG1066191

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: ACF0KO_RS01825
• Genome accession: NZ_CP172307
• Coordinates: 419326..420387 (+)
• Length: 353 a.a.
• NCBI ID: WP_064024049.1
• Uniprot ID: A0A1S1Y3S6
• Organism: Methylomonas sp. 2B
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 414326..425387

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACF0KO_RS01805 (ACF0KO_01805)	hisG	414398..415030 (+)	633	WP_064024025.1	ATP phosphoribosyltransferase	-
ACF0KO_RS01810 (ACF0KO_01810)	hisD	415027..416328 (+)	1302	WP_064024027.1	histidinol dehydrogenase	-
ACF0KO_RS01815 (ACF0KO_01815)	rpmE	416485..416685 (+)	201	WP_082885273.1	50S ribosomal protein L31	-
ACF0KO_RS01820 (ACF0KO_01820)	-	416779..419091 (-)	2313	WP_231883508.1	penicillin-binding protein 1A	-
ACF0KO_RS01825 (ACF0KO_01825)	pilM	419326..420387 (+)	1062	WP_064024049.1	pilus assembly protein PilM	Machinery gene
ACF0KO_RS01830 (ACF0KO_01830)	-	420387..420959 (+)	573	WP_064024031.1	PilN domain-containing protein	-
ACF0KO_RS01835 (ACF0KO_01835)	-	420956..421567 (+)	612	WP_064024033.1	type 4a pilus biogenesis protein PilO	-
ACF0KO_RS01840 (ACF0KO_01840)	-	421570..422130 (+)	561	WP_082885274.1	pilus assembly protein PilP	-
ACF0KO_RS01845 (ACF0KO_01845)	pilQ	422133..424391 (+)	2259	WP_064024037.1	type IV pilus secretin family protein	-
ACF0KO_RS01850 (ACF0KO_01850)	-	424503..424619 (+)	117	WP_139306267.1	lipoprotein	-

Sequence

Protein

• Length: 353 a.a.
• Molecular weight: 37952.47 Da
• Isoelectric Point: 4.8032

>NTDB_id=1066191 ACF0KO_RS01825 WP_064024049.1 419326..420387(+) (pilM) [Methylomonas sp. 2B]
MSWFSSGQSAVLGVDISTAAVKLLELSRVGARYKVESYAVAPLPQDAIVDKNITNVEQIGSVIKTAVKQSGTRAKHACVA
VAGSSVMTKIISMPASLSEAEMEEQIMVEADQYIPYSLDEVNLDFEVQGATKANPQMVDVLLAASRRENIEDRVAALAYA
GLKAVVVDVEAFAMENAFKLLADQLPEAVEHKTVAIADIGATMTSLNILHDGKTVYTREQGFGGKQLTEEIQRRYGLSYE
EAGLAKKHGGLPDNYVSDVLEPFKKAMLQQIARSLQFFVSSSANRDVDALILAGGCASIAGLDKLVERDLGIASYIANPF
INMALSNKVKPQSLNNDTPAMMIACGLALRSFD

Nucleotide

• Length: 1062 bp

>NTDB_id=1066191 ACF0KO_RS01825 WP_064024049.1 419326..420387(+) (pilM) [Methylomonas sp. 2B]
ATGAGCTGGTTTAGCAGTGGTCAATCGGCTGTGCTCGGTGTGGATATTAGCACCGCGGCGGTAAAATTGTTGGAGTTAAG
CCGGGTGGGCGCGCGTTACAAAGTCGAGAGCTATGCGGTGGCGCCTTTGCCGCAAGACGCTATCGTCGATAAGAACATTA
CTAACGTAGAGCAAATCGGCAGTGTGATCAAGACCGCCGTCAAGCAATCCGGAACGCGCGCCAAGCACGCTTGCGTGGCG
GTGGCCGGTTCGTCGGTGATGACTAAAATCATTTCCATGCCGGCGTCGTTGTCGGAGGCGGAAATGGAAGAGCAAATCAT
GGTCGAGGCCGATCAGTACATTCCGTATTCCTTGGACGAGGTTAATCTTGATTTCGAAGTGCAGGGCGCTACCAAGGCCA
ACCCGCAAATGGTCGATGTGTTGTTAGCGGCGTCCAGACGTGAAAATATTGAAGACCGAGTGGCGGCCTTGGCTTACGCC
GGACTAAAGGCGGTCGTTGTCGATGTCGAGGCTTTTGCGATGGAAAACGCTTTCAAATTGTTGGCCGATCAATTGCCGGA
GGCTGTCGAACACAAGACGGTTGCGATTGCCGATATCGGCGCCACGATGACCTCGCTGAATATTCTGCATGACGGCAAGA
CCGTCTATACCCGGGAACAAGGCTTTGGCGGCAAACAGTTAACCGAGGAAATACAGCGTCGCTACGGCTTGTCTTACGAA
GAGGCGGGGTTGGCGAAAAAGCACGGCGGCTTGCCGGACAACTACGTCAGCGATGTTCTGGAACCTTTCAAAAAAGCCAT
GCTGCAACAAATTGCCAGATCGTTACAGTTTTTTGTGTCTTCCAGCGCTAACCGGGATGTCGATGCGTTGATCTTGGCCG
GCGGTTGCGCTTCGATAGCCGGTTTGGACAAATTGGTGGAACGTGATTTGGGCATCGCTTCCTATATCGCCAACCCCTTC
ATCAACATGGCTCTGTCCAACAAGGTTAAGCCGCAGAGCCTGAATAACGATACTCCGGCGATGATGATCGCTTGCGGTTT
GGCGCTGAGGAGCTTCGACTGA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (13-352)

3D structure

• AlphaFold DB: A0A1S1Y3S6

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	50.708	100	0.507
comM	Acinetobacter nosocomialis M2	50.142	100	0.501
comM	Acinetobacter baylyi ADP1	48.725	100	0.487
pilM	Legionella pneumophila strain ERS1305867	41.926	100	0.419