Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   ACH6CW_RS29175 Genome accession   NZ_CP172306
Coordinates   6241206..6242270 (-) Length   354 a.a.
NCBI ID   WP_003110888.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain pat0103     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6236206..6247270
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACH6CW_RS29150 aroK 6236741..6237259 (-) 519 WP_003095833.1 shikimate kinase AroK -
  ACH6CW_RS29155 pilQ 6237271..6239415 (-) 2145 WP_012614629.1 type 4a pilus secretin PilQ Machinery gene
  ACH6CW_RS29160 pilP 6239469..6239993 (-) 525 WP_003095836.1 type 4a pilus biogenesis lipoprotein PilP -
  ACH6CW_RS29165 pilO 6239990..6240613 (-) 624 WP_003103268.1 type 4a pilus biogenesis protein PilO -
  ACH6CW_RS29170 pilN 6240610..6241206 (-) 597 WP_003095839.1 type 4a pilus biogenesis protein PilN -
  ACH6CW_RS29175 pilM 6241206..6242270 (-) 1065 WP_003110888.1 type IV pilus assembly protein PilM Machinery gene
  ACH6CW_RS29180 - 6242455..6244926 (+) 2472 WP_260550825.1 penicillin-binding protein 1A -
  ACH6CW_RS29185 - 6245022..6246290 (-) 1269 WP_003095842.1 malic enzyme -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 37983.48 Da        Isoelectric Point: 4.5405

>NTDB_id=1066181 ACH6CW_RS29175 WP_003110888.1 6241206..6242270(-) (pilM) [Pseudomonas aeruginosa strain pat0103]
MLGLIKKKANTLLGIDISSTSVKLLELSRSGGRYKVEAYAVEPLPPNAVVEKNIVELEGVGQALSRVLVKAKTNLKSAVV
AVAGSAVITKTIEMEAGLSEDELENQLKIEADQYIPYPLEEVAIDFEVQGLSARNPERVDVLLAACRKENVEVREAALAL
AGLTAKVVDVEAYALERSYALLSSQLGADTDQLTVAVVDIGATMTTLSVLHNGRTIYTREQLFGGRQLTEEIQRRYGLSV
EEAGLAKKQGGLPDDYDSEVLRPFKDAVVQQVSRSLQFFFAAGQFNDVDYIVLAGGTASIQDLDRLIQQKIGTPTLVANP
FADMALNGKVNAGALASDAPALMIACGLALRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=1066181 ACH6CW_RS29175 WP_003110888.1 6241206..6242270(-) (pilM) [Pseudomonas aeruginosa strain pat0103]
GTGCTAGGGCTCATAAAGAAGAAAGCGAACACCTTGCTGGGGATCGACATCAGCTCGACCTCGGTAAAGCTCCTTGAGTT
GAGCCGCTCCGGAGGCCGCTACAAAGTGGAGGCCTATGCCGTCGAGCCGCTTCCGCCGAATGCAGTCGTGGAAAAGAACA
TCGTCGAGCTGGAAGGGGTCGGCCAGGCGTTGTCGCGGGTGCTGGTCAAGGCGAAAACCAACCTGAAGTCGGCCGTGGTC
GCCGTCGCCGGTTCGGCGGTGATCACCAAGACCATCGAGATGGAGGCCGGGCTTTCCGAGGATGAACTGGAAAACCAGCT
GAAGATCGAGGCCGACCAGTACATCCCCTATCCGCTGGAGGAAGTCGCCATCGACTTCGAGGTCCAGGGGCTCTCCGCGC
GCAATCCCGAGCGCGTGGACGTACTCCTGGCCGCCTGTCGGAAAGAGAACGTCGAGGTTCGCGAAGCGGCCCTGGCGCTG
GCCGGCCTGACCGCCAAGGTGGTCGACGTCGAGGCCTACGCGCTGGAGCGCTCCTATGCGCTGCTGAGCAGCCAGCTGGG
TGCCGACACCGACCAGTTGACCGTGGCGGTAGTGGACATCGGCGCGACCATGACCACCCTGAGCGTCCTGCACAACGGAC
GCACCATCTATACCCGCGAACAGCTGTTCGGCGGTCGCCAGCTCACCGAGGAGATCCAGCGGCGTTACGGACTCTCGGTG
GAGGAAGCCGGTCTCGCCAAGAAGCAGGGCGGTCTTCCGGATGACTACGACAGCGAGGTTCTGCGTCCGTTCAAGGACGC
CGTGGTGCAGCAGGTTTCCCGCTCGCTGCAGTTCTTCTTCGCCGCCGGACAGTTCAACGACGTCGACTACATCGTCCTGG
CGGGCGGTACGGCGTCTATCCAGGATCTCGATCGGTTGATCCAGCAGAAGATCGGCACCCCGACCCTGGTCGCCAACCCG
TTCGCCGACATGGCGCTGAACGGCAAGGTGAATGCCGGCGCCCTGGCCAGCGACGCTCCGGCGTTGATGATCGCCTGCGG
CCTGGCGTTGAGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

55.899

100

0.562
  comM Acinetobacter nosocomialis M2

55.899

100

0.562
  comM Acinetobacter baylyi ADP1

51.977

100

0.52
  pilM Legionella pneumophila strain ERS1305867

46.61

100

0.466

Multiple sequence alignment