Detailed information NTG1066153

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: ACH6CW_RS02015
• Genome accession: NZ_CP172306
• Coordinates: 434381..435529 (+)
• Length: 382 a.a.
• NCBI ID: WP_003110016.1
• Uniprot ID: A0A0H2ZL88
• Organism: Pseudomonas aeruginosa strain pat0103
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 429381..440529

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACH6CW_RS01990	-	430532..430828 (-)	297	WP_031628111.1	DUF167 domain-containing protein	-
ACH6CW_RS01995	-	430825..431418 (-)	594	WP_003084544.1	YggT family protein	-
ACH6CW_RS02000	proC	431429..432250 (-)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
ACH6CW_RS02005	-	432262..432954 (-)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
ACH6CW_RS02010	pilT	433169..434203 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
ACH6CW_RS02015	pilU	434381..435529 (+)	1149	WP_003110016.1	type IV pilus ATPase PilU	Machinery gene
ACH6CW_RS02020	czcD	435537..436436 (-)	900	WP_023913256.1	CDF family zinc transporter CzcD	-
ACH6CW_RS02025	-	436590..436994 (+)	405	WP_003084560.1	NINE protein	-
ACH6CW_RS02030	-	437237..438610 (+)	1374	WP_003084562.1	pyridoxal-phosphate dependent enzyme	-
ACH6CW_RS02035	-	438607..439791 (+)	1185	WP_023092310.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42559.83 Da
• Isoelectric Point: 6.7792

>NTDB_id=1066153 ACH6CW_RS02015 WP_003110016.1 434381..435529(+) (pilU) [Pseudomonas aeruginosa strain pat0103]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSNLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=1066153 ACH6CW_RS02015 WP_003110016.1 434381..435529(+) (pilU) [Pseudomonas aeruginosa strain pat0103]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACTCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGATCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAACCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: A0A0H2ZL88

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.22	100	0.882
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382