Detailed information NTG0106549

Bioinformatically predicted

Overview

• Name: comGF
• Type: Machinery gene
• Locus tag: EN71_RS01120
• Genome accession: NZ_CP007631
• Coordinates: 190926..191387 (+)
• Length: 153 a.a.
• NCBI ID: WP_001878820.1
• Uniprot ID: -
• Organism: Streptococcus agalactiae strain NGBS061
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 185926..196387

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
EN71_RS01085 (EN72_01105)	-	186680..187045 (+)	366	WP_000285373.1	DUF1033 family protein	-
EN71_RS11255 (EN72_01110)	comGA	187218..188189 (+)	972	WP_000250805.1	competence type IV pilus ATPase ComGA	Machinery gene
EN71_RS01090 (EN72_01115)	comGB	188083..189126 (+)	1044	WP_223295889.1	competence type IV pilus assembly protein ComGB	Machinery gene
EN71_RS01095 (EN72_01120)	comGC/cglC	189123..189452 (+)	330	WP_000793380.1	competence type IV pilus major pilin ComGC	Machinery gene
EN71_RS11820	-	189427..189537 (+)	111	Protein_164	competence protein	-
EN71_RS01110 (EN72_01135)	comGD	190384..190701 (+)	318	Protein_166	competence type IV pilus minor pilin ComGD	-
EN71_RS01115 (EN72_01140)	comGE	190673..190972 (+)	300	WP_000234804.1	competence type IV pilus minor pilin ComGE	Machinery gene
EN71_RS01120 (EN72_01145)	comGF	190926..191387 (+)	462	WP_001878820.1	competence type IV pilus minor pilin ComGF	Machinery gene
EN71_RS01125 (EN72_01150)	comGG	191365..191736 (+)	372	WP_000601104.1	competence type IV pilus minor pilin ComGG	Machinery gene
EN71_RS01130 (EN72_01155)	comGH	191851..192825 (+)	975	WP_001008574.1	class I SAM-dependent methyltransferase	Machinery gene
EN71_RS01135 (EN72_01160)	-	192857..194050 (+)	1194	WP_000047534.1	acetate kinase	-
EN71_RS01140 (EN72_01165)	-	194202..194408 (+)	207	WP_000798241.1	helix-turn-helix transcriptional regulator	-
EN71_RS11300	-	194467..194604 (+)	138	WP_001865900.1	hypothetical protein	-
EN71_RS01145 (EN72_01170)	-	194645..195100 (+)	456	WP_000905673.1	hypothetical protein	-
EN71_RS01150 (EN72_01175)	-	195169..195834 (+)	666	WP_000008113.1	CPBP family intramembrane glutamic endopeptidase	-

Sequence

Protein

• Length: 153 a.a.
• Molecular weight: 17874.95 Da
• Isoelectric Point: 10.0299

>NTDB_id=106549 EN71_RS01120 WP_001878820.1 190926..191387(+) (comGF) [Streptococcus agalactiae strain NGBS061]
MKIIRKFCTYQKLRVKAFTLLKCLVALVTITGALLVYQGLTKLLAQQIVVMSSSSQSEWVLLTQQLNAEFEGAHLEYLRQ
NKLYLRKQDKIVTFGKSNKDDFRKTGYDGRGYQPMVYGLDNCQMSQTKSMVKLVFYFKDGLKRTFYYDFKEET

Nucleotide

• Length: 462 bp

>NTDB_id=106549 EN71_RS01120 WP_001878820.1 190926..191387(+) (comGF) [Streptococcus agalactiae strain NGBS061]
TTGAAGATAATAAGGAAATTTTGCACGTATCAAAAGTTAAGGGTTAAAGCATTTACGCTTTTAAAATGTTTGGTAGCATT
GGTTACAATCACAGGAGCTTTACTAGTTTATCAAGGACTGACAAAATTGTTGGCTCAACAGATAGTAGTGATGTCTTCTT
CCAGTCAGTCTGAATGGGTGTTATTAACTCAGCAACTAAATGCAGAATTTGAAGGCGCTCATCTGGAATATTTAAGACAG
AACAAACTTTATTTACGTAAGCAAGATAAGATTGTAACCTTTGGCAAATCTAATAAAGATGATTTCCGTAAGACAGGTTA
TGATGGTCGAGGTTATCAACCAATGGTTTATGGGTTAGACAATTGTCAAATGAGTCAGACCAAAAGTATGGTAAAACTTG
TTTTTTATTTTAAGGACGGGTTAAAAAGGACATTTTACTATGATTTTAAAGAAGAAACTTAA

Domains

Predicted by InterProScan.

• Putative Competence protein ComGF (ComGF) (49-142)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comGF	Streptococcus mutans UA159	47.183	92.81	0.438
comGF	Streptococcus mutans UA140	47.183	92.81	0.438
comGF/cglF	Streptococcus pneumoniae Rx1	42.446	90.85	0.386
comGF/cglF	Streptococcus pneumoniae D39	42.446	90.85	0.386
comGF/cglF	Streptococcus pneumoniae R6	42.446	90.85	0.386
comGF/cglF	Streptococcus pneumoniae TIGR4	42.446	90.85	0.386
comGF/cglF	Streptococcus mitis SK321	43.284	87.582	0.379
comGF/cglF	Streptococcus mitis NCTC 12261	42.537	87.582	0.373
comGF	Lactococcus lactis subsp. cremoris KW2	40	91.503	0.366