Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   DP32_RS19495 Genome accession   NZ_CP007594
Coordinates   3852017..3852694 (+) Length   225 a.a.
NCBI ID   WP_000178161.1    Uniprot ID   -
Organism   Escherichia coli strain SEC470     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3847017..3857694
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DP32_RS19460 (DP32_19490) gspG 3847497..3847934 (+) 438 WP_001202874.1 type II secretion system major pseudopilin GspG -
  DP32_RS19465 (DP32_19495) gspH 3847942..3848451 (+) 510 WP_001076046.1 type II secretion system minor pseudopilin GspH -
  DP32_RS19470 (DP32_19500) gspI 3848448..3848825 (+) 378 WP_001041743.1 type II secretion system minor pseudopilin GspI -
  DP32_RS19475 (DP32_19505) gspJ 3848818..3849405 (+) 588 WP_000610634.1 type II secretion system minor pseudopilin GspJ -
  DP32_RS19480 (DP32_19510) gspK 3849398..3850381 (+) 984 WP_001058522.1 type II secretion system minor pseudopilin GspK -
  DP32_RS19485 (DP32_19515) gspL 3850396..3851559 (+) 1164 WP_001115238.1 type II secretion system protein GspL -
  DP32_RS19490 (DP32_19520) gspM 3851556..3852017 (+) 462 WP_014640026.1 type II secretion system protein GspM -
  DP32_RS19495 (DP32_19525) pilD 3852017..3852694 (+) 678 WP_000178161.1 prepilin peptidase GspO Machinery gene
  DP32_RS19500 (DP32_19530) bfr 3852723..3853199 (-) 477 WP_000675504.1 bacterioferritin -
  DP32_RS19505 (DP32_19535) bfd 3853271..3853465 (-) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  DP32_RS19510 (DP32_19540) chiA 3853634..3856327 (-) 2694 WP_000773156.1 bifunctional chitinase/lysozyme -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24898.78 Da        Isoelectric Point: 7.9563

>NTDB_id=106330 DP32_RS19495 WP_000178161.1 3852017..3852694(+) (pilD) [Escherichia coli strain SEC470]
MTMLLPLFILVGFIAGYFVNAIAYHLSPLEDKTALTFRQVLVHFRQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFVLTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQYGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=106330 DP32_RS19495 WP_000178161.1 3852017..3852694(+) (pilD) [Escherichia coli strain SEC470]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGGTTATTTTGTTAATGCCATCGCCTATCACCTCTC
GCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGTACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTATGGATTAATTGATTTACATGATGCGGTTTACGGCGCGGTAGCGG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGGTATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

32.946

100

0.378

Multiple sequence alignment