Detailed information NTG1061408

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: ACGB44_RS20095
• Genome accession: NZ_CP171478
• Coordinates: 4289528..4290673 (-)
• Length: 381 a.a.
• NCBI ID: WP_026006585.1
• Uniprot ID: A0AA47I1L4
• Organism: Stutzerimonas frequens strain RL-XB02
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4284528..4295673

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACGB44_RS20070 (ACGB44_20070)	-	4284608..4285624 (+)	1017	WP_063540691.1	aspartate carbamoyltransferase catalytic subunit	-
ACGB44_RS20075 (ACGB44_20075)	-	4285624..4286898 (+)	1275	WP_110778830.1	dihydroorotase	-
ACGB44_RS20080 (ACGB44_20080)	-	4286955..4287350 (-)	396	WP_017245432.1	TM2 domain-containing protein	-
ACGB44_RS20085 (ACGB44_20085)	-	4287483..4288697 (+)	1215	WP_394375103.1	putative DNA modification/repair radical SAM protein	-
ACGB44_RS20090 (ACGB44_20090)	-	4288697..4289521 (+)	825	WP_394375104.1	TIGR03915 family putative DNA repair protein	-
ACGB44_RS20095 (ACGB44_20095)	pilU	4289528..4290673 (-)	1146	WP_026006585.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
ACGB44_RS20100 (ACGB44_20100)	pilT	4290734..4291768 (-)	1035	WP_017245436.1	type IV pilus twitching motility protein PilT	Machinery gene
ACGB44_RS20105 (ACGB44_20105)	-	4291835..4292524 (+)	690	WP_267935792.1	YggS family pyridoxal phosphate-dependent enzyme	-
ACGB44_RS20110 (ACGB44_20110)	proC	4292535..4293359 (+)	825	WP_200597334.1	pyrroline-5-carboxylate reductase	-
ACGB44_RS20115 (ACGB44_20115)	-	4293369..4293959 (+)	591	WP_017245439.1	YggT family protein	-

Sequence

Protein

• Length: 381 a.a.
• Molecular weight: 42540.84 Da
• Isoelectric Point: 6.8856

>NTDB_id=1061408 ACGB44_RS20095 WP_026006585.1 4289528..4290673(-) (pilU) [Stutzerimonas frequens strain RL-XB02]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGKIMPVSKTAMSPEMTRETVHGVMNEQQRREFTENHECNFAISARGIGR
FRVSAFYQRNLAGMVLRRIETNIPTIEELKLPDVLKKLSMTKRGLVLFVGATGTGKSTSLASMIGYRNKNSSGHIISIED
PIEFIHQHQNCIVTQREVGIDTSSFEVALKNTLRQAPDVILIGEIRTRETMDYAVAFAETGHLCLATLHANNANQALDRI
INFFPPDRHNQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLINTPLAADLIRKGEVHELKSLMKRSTELGMQTFDQA
LYNLYVQGEITYEDALLHADSANDLRLLIKLGSETDGEHLTTVSQGLSLEVSDDDPGRRFR

Nucleotide

• Length: 1146 bp

>NTDB_id=1061408 ACGB44_RS20095 WP_026006585.1 4289528..4290673(-) (pilU) [Stutzerimonas frequens strain RL-XB02]
ATGGAATTCGAGAAACTTCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGATCTGTTCATCACGGCGGGCGTGCCGCCGTC
GATGAAGGTCAACGGCAAGATCATGCCGGTGAGCAAGACCGCCATGTCGCCGGAAATGACCCGCGAGACCGTGCACGGCG
TGATGAACGAGCAGCAGCGCCGCGAGTTCACCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGTATCGGCCGT
TTCCGGGTCAGTGCGTTCTACCAGCGCAACCTGGCCGGCATGGTGCTGCGGCGTATCGAAACGAACATTCCGACCATCGA
AGAGCTGAAGCTGCCTGACGTCCTCAAGAAGCTGTCGATGACCAAGCGTGGCCTGGTGCTGTTCGTCGGCGCCACCGGCA
CTGGCAAGTCGACCTCGCTGGCCTCGATGATCGGCTACCGCAACAAGAACTCCAGCGGCCACATCATCTCCATCGAAGAC
CCGATCGAGTTCATCCACCAGCATCAGAACTGCATCGTTACCCAGCGCGAAGTCGGCATCGATACCAGCTCTTTCGAGGT
GGCACTGAAGAACACGCTGCGCCAGGCGCCGGACGTGATTCTCATCGGCGAGATTCGTACCCGCGAGACGATGGACTATG
CCGTAGCCTTCGCCGAAACCGGACACCTGTGTCTGGCCACGCTGCACGCCAACAATGCCAACCAGGCGCTGGACCGCATC
ATCAACTTCTTCCCACCGGATCGGCACAACCAGGTCTGGATGGATCTGTCGCTCAACCTCAAGGCCATCGTCGCCCAACA
GCTGGTGCCGACACCCGACGGCAAGGGTCGTCGCGCGGTGATCGAGGTGTTGATCAACACCCCTCTGGCCGCAGACCTTA
TCCGCAAGGGCGAGGTGCATGAACTCAAGTCGCTGATGAAACGCTCCACCGAGCTGGGTATGCAGACCTTCGACCAGGCG
CTGTACAACCTCTATGTGCAGGGCGAGATCACCTATGAAGATGCGCTGTTGCACGCCGACTCGGCCAACGACCTGCGGCT
GTTGATCAAGCTGGGTTCGGAAACCGACGGCGAGCACCTGACCACTGTTTCGCAGGGGTTGAGTCTGGAGGTCAGCGACG
ACGATCCGGGTCGGCGCTTCCGTTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (113-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	94.751	100	0.948
pilU	Acinetobacter baylyi ADP1	66.106	93.701	0.619
pilU	Vibrio cholerae strain A1552	59.714	91.864	0.549
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.658	88.976	0.388