Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   ACEPLV_RS26280 Genome accession   NZ_CP171230
Coordinates   5669083..5670147 (-) Length   354 a.a.
NCBI ID   WP_034074518.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain PAXJ     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5664083..5675147
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACEPLV_RS26255 (ACEPLV_26255) aroK 5664618..5665136 (-) 519 WP_003095833.1 shikimate kinase AroK -
  ACEPLV_RS26260 (ACEPLV_26260) pilQ 5665148..5667292 (-) 2145 WP_003146401.1 type 4a pilus secretin PilQ Machinery gene
  ACEPLV_RS26265 (ACEPLV_26265) pilP 5667346..5667870 (-) 525 WP_003095836.1 type 4a pilus biogenesis lipoprotein PilP -
  ACEPLV_RS26270 (ACEPLV_26270) pilO 5667867..5668490 (-) 624 WP_003103268.1 type 4a pilus biogenesis protein PilO -
  ACEPLV_RS26275 (ACEPLV_26275) pilN 5668487..5669083 (-) 597 WP_003095839.1 type 4a pilus biogenesis protein PilN -
  ACEPLV_RS26280 (ACEPLV_26280) pilM 5669083..5670147 (-) 1065 WP_034074518.1 type IV pilus assembly protein PilM Machinery gene
  ACEPLV_RS26285 (ACEPLV_26285) - 5670332..5672803 (+) 2472 WP_003161248.1 penicillin-binding protein 1A -
  ACEPLV_RS26290 (ACEPLV_26290) - 5672908..5674176 (-) 1269 WP_003103278.1 malic enzyme -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 37968.51 Da        Isoelectric Point: 4.5405

>NTDB_id=1060591 ACEPLV_RS26280 WP_034074518.1 5669083..5670147(-) (pilM) [Pseudomonas aeruginosa strain PAXJ]
MLGLIKKKANTLLGIDISSTSVKLLELSRSGGRYKVEAYAVEPLPPNAVVEKNIVELEGVGLALSRVLVKAKTNLKSAVV
AVAGSAVITKTIEMEAGLSEDELENQLKIEADQYIPYPLEEVAIDFEVQGLSARNPERVDVLLAACRKENVEVREAALAL
AGLTAKVVDVEAYALERSYALLSSQLGADTDQLTVAVVDIGATMTTLSVLHNGRTIYTREQLFGGRQLTEEIQRRYGLSV
EEAGLAKKQGGLPDDYDSEVLRPFKDAVVQQVSRSLQFFFAAGQFNDVDYIVLAGGTASIQDLDRLIQQKIGTPTLVANP
FADMALNGKVNAGALASDAPALMIACGLALRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=1060591 ACEPLV_RS26280 WP_034074518.1 5669083..5670147(-) (pilM) [Pseudomonas aeruginosa strain PAXJ]
GTGCTAGGGCTCATAAAGAAGAAAGCGAACACCTTGCTGGGGATCGACATCAGCTCGACCTCGGTAAAGCTCCTTGAGTT
GAGCCGCTCCGGAGGCCGCTACAAAGTGGAGGCCTATGCCGTCGAGCCGCTTCCGCCGAATGCAGTCGTGGAAAAGAACA
TCGTCGAGCTGGAAGGGGTCGGCCTGGCGTTGTCACGGGTGCTGGTCAAGGCGAAAACCAACCTGAAGTCGGCCGTGGTC
GCCGTCGCCGGTTCGGCGGTGATCACCAAGACCATCGAGATGGAGGCCGGGCTTTCCGAGGATGAACTGGAAAACCAGCT
GAAGATCGAGGCCGACCAGTACATCCCCTATCCGCTGGAGGAAGTCGCCATCGACTTCGAGGTCCAGGGGCTCTCCGCGC
GCAATCCCGAGCGCGTGGACGTACTCCTGGCCGCCTGTCGGAAAGAGAACGTCGAGGTTCGCGAAGCGGCCCTGGCGCTG
GCCGGCCTGACCGCCAAGGTGGTCGATGTCGAGGCCTACGCGCTGGAGCGCTCCTATGCGCTGCTGAGCAGCCAGCTGGG
TGCCGACACCGACCAGTTGACCGTGGCGGTAGTGGACATCGGCGCGACCATGACCACCCTGAGCGTCCTGCACAACGGAC
GCACCATCTATACCCGCGAACAGCTATTCGGCGGTCGCCAGCTCACCGAGGAGATCCAGCGGCGTTACGGACTCTCGGTG
GAGGAAGCCGGTCTCGCCAAGAAGCAGGGCGGTCTTCCGGATGACTACGACAGCGAAGTTCTGCGTCCGTTCAAGGACGC
CGTGGTGCAGCAGGTTTCCCGCTCGCTGCAGTTCTTCTTCGCCGCCGGACAGTTCAACGACGTCGACTACATCGTCCTGG
CGGGCGGTACGGCGTCTATCCAGGATCTCGATCGGTTGATCCAGCAGAAGATCGGCACCCCGACCCTGGTCGCCAACCCG
TTCGCCGACATGGCGCTGAACGGCAAGGTGAATGCCGGCGCCCTGGCCAGCGACGCTCCGGCGTTGATGATCGCCTGCGG
CCTGGCGTTGAGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

55.899

100

0.562
  comM Acinetobacter nosocomialis M2

55.899

100

0.562
  comM Acinetobacter baylyi ADP1

51.977

100

0.52
  pilM Legionella pneumophila strain ERS1305867

46.61

100

0.466

Multiple sequence alignment