Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF   Type   Machinery gene
Locus tag   DX05_RS01270 Genome accession   NZ_CP007572
Coordinates   219976..220431 (+) Length   151 a.a.
NCBI ID   WP_000793378.1    Uniprot ID   Q8E234
Organism   Streptococcus agalactiae strain GBS6     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 214976..225431
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DX05_RS01245 (DX05_01265) - 215059..216471 (-) 1413 WP_017771557.1 IS1182 family transposase -
  DX05_RS01250 (DX05_01270) comGA 216636..217607 (+) 972 WP_000250798.1 competence type IV pilus ATPase ComGA Machinery gene
  DX05_RS01255 (DX05_01275) comGB 217501..218448 (+) 948 Protein_197 competence type IV pilus assembly protein ComGB -
  DX05_RS01270 (DX05_01290) comGF 219976..220431 (+) 456 WP_000793378.1 competence type IV pilus minor pilin ComGF Machinery gene
  DX05_RS01275 (DX05_01295) comGG 220409..220780 (+) 372 WP_000601101.1 competence type IV pilus minor pilin ComGG Machinery gene
  DX05_RS01280 (DX05_01300) comGH 220895..221869 (+) 975 WP_001008569.1 class I SAM-dependent methyltransferase Machinery gene
  DX05_RS01285 (DX05_01305) - 221901..223094 (+) 1194 WP_000047534.1 acetate kinase -
  DX05_RS01290 - 223222..223414 (+) 193 Protein_203 helix-turn-helix transcriptional regulator -
  DX05_RS11170 - 223393..223659 (+) 267 Protein_204 hypothetical protein -
  DX05_RS01295 (DX05_01315) - 223728..224393 (+) 666 WP_000008108.1 type II CAAX endopeptidase family protein -
  DX05_RS01300 (DX05_01320) proC 224414..225184 (-) 771 WP_001881026.1 pyrroline-5-carboxylate reductase -

Sequence

Protein


Download         Length: 151 a.a.        Molecular weight: 17509.47 Da        Isoelectric Point: 9.7378

>NTDB_id=105989 DX05_RS01270 WP_000793378.1 219976..220431(+) (comGF) [Streptococcus agalactiae strain GBS6]
MKNLLLKCKDKKVKAFTLLECLVALVTITGALLVYQGLTKLLAQQIVVMSSSSQSEWVLLTQQLNAEFEGAHLEYLRQNK
LYLRKQDKIVTFGKSNKDDFRKTGYDGRGYQPMVYGLDNCQMSQTKSMVKLVFYFKDGLKRTFYYDFKEET

Nucleotide


Download         Length: 456 bp        

>NTDB_id=105989 DX05_RS01270 WP_000793378.1 219976..220431(+) (comGF) [Streptococcus agalactiae strain GBS6]
ATGAAAAATTTATTGTTAAAATGTAAGGATAAGAAGGTTAAAGCATTTACACTTTTAGAATGTTTGGTAGCATTGGTTAC
AATCACAGGAGCTTTGCTAGTTTATCAAGGACTAACAAAATTGTTGGCTCAACAGATAGTAGTGATGTCTTCTTCCAGTC
AGTCTGAATGGGTGTTATTAACTCAGCAACTAAATGCAGAATTTGAAGGCGCTCATCTGGAATATTTAAGACAGAACAAA
CTTTATTTACGTAAGCAAGATAAGATTGTAACCTTTGGCAAATCTAATAAAGATGATTTCCGTAAGACAGGTTATGATGG
TCGAGGTTATCAACCAATGGTTTATGGGTTAGACAATTGTCAAATGAGTCAGACCAAAAGTATGGTAAAACTTGTTTTTT
ATTTTAAGGACGGGTTAAAAAGGACATTTTACTATGATTTTAAAGAAGAAACTTAA

Domains

Predicted by InterProScan.

Putative Competence protein ComGF (ComGF)

(47-140)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q8E234

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF Streptococcus mutans UA159

48.571

92.715

0.45
  comGF Streptococcus mutans UA140

48.571

92.715

0.45
  comGF/cglF Streptococcus pneumoniae Rx1

42

99.338

0.417
  comGF/cglF Streptococcus pneumoniae D39

42

99.338

0.417
  comGF/cglF Streptococcus pneumoniae R6

42

99.338

0.417
  comGF/cglF Streptococcus pneumoniae TIGR4

42

99.338

0.417
  comGF/cglF Streptococcus mitis SK321

44.203

91.391

0.404
  comGF/cglF Streptococcus mitis NCTC 12261

43.478

91.391

0.397
  comGF Lactococcus lactis subsp. cremoris KW2

40.58

91.391

0.371

Multiple sequence alignment