Detailed information    

insolico Bioinformatically predicted

Overview

Name   endA   Type   Machinery gene
Locus tag   AAA985_RS09945 Genome accession   NZ_AP028603
Coordinates   1899979..1900803 (-) Length   274 a.a.
NCBI ID   WP_001036785.1    Uniprot ID   -
Organism   Streptococcus pneumoniae strain Pne2     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 1894979..1905803
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AAA985_RS09940 (TKY181970_19520) - 1898357..1899688 (-) 1332 WP_000390044.1 hemolysin family protein -
  AAA985_RS09945 (TKY181970_19530) endA 1899979..1900803 (-) 825 WP_001036785.1 DNA-entry nuclease EndA Machinery gene
  AAA985_RS09950 (TKY181970_19540) - 1900842..1901030 (-) 189 WP_001036519.1 DNA-directed RNA polymerase subunit beta -
  AAA985_RS09955 (TKY181970_19550) murA 1901023..1902306 (-) 1284 WP_000358028.1 UDP-N-acetylglucosamine 1-carboxyvinyltransferase -
  AAA985_RS09960 (TKY181970_19560) - 1902330..1902560 (-) 231 WP_000250391.1 DUF1146 family protein -
  AAA985_RS09965 (TKY181970_19570) sepM 1902618..1903655 (-) 1038 WP_000727277.1 SepM family pheromone-processing serine protease Regulator
  AAA985_RS09970 (TKY181970_19580) coaD 1903639..1904127 (-) 489 WP_001280746.1 pantetheine-phosphate adenylyltransferase -
  AAA985_RS09975 (TKY181970_19590) rsmD 1904117..1904656 (-) 540 WP_000691938.1 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD -
  AAA985_RS09980 (TKY181970_19600) asnA 1904721..1905713 (-) 993 WP_000747993.1 aspartate--ammonia ligase -

Sequence

Protein


Download         Length: 274 a.a.        Molecular weight: 29917.56 Da        Isoelectric Point: 10.0650

>NTDB_id=105991 AAA985_RS09945 WP_001036785.1 1899979..1900803(-) (endA) [Streptococcus pneumoniae strain Pne2]
MNKKTRQTLIGLLVLLLLSTGSYYIKQMQSAPNSPKTNLSQKKQAPEAPSQALAESVLTDAVKSQIKGSLEWNGSGAFIV
NGNKTNLDAKVSSKPYADNKTKTVGKETVPTVANALLSNATRQYKNRKETGNGSTSWTPPGWHQVKNLKGSYTHAVDRGH
LLGYALIGGLDGFDASTSNPKNIAVQTAWANQAQAEYSTGQNYYESKVRKALDQNKRVRYRVTLYYASNEDLVPSASQIE
AKSSDGELEFNVLVPNVQKGLQLDYRTGEVTVTQ

Nucleotide


Download         Length: 825 bp        

>NTDB_id=105991 AAA985_RS09945 WP_001036785.1 1899979..1900803(-) (endA) [Streptococcus pneumoniae strain Pne2]
ATGAACAAAAAAACAAGACAGACACTAATCGGACTGCTAGTGTTATTGCTTTTGTCTACAGGGAGCTATTATATCAAGCA
GATGCAGTCGGCACCTAATAGTCCCAAAACCAATCTTAGTCAGAAAAAACAAGCGCCTGAAGCTCCTAGTCAAGCATTGG
CAGAGAGTGTCTTAACAGACGCAGTCAAGAGTCAAATAAAGGGGAGTCTGGAGTGGAATGGCTCAGGTGCTTTTATCGTC
AATGGTAATAAAACAAATCTAGATGCCAAGGTTTCAAGTAAGCCCTACGCTGACAATAAAACAAAGACAGTGGGCAAGGA
AACTGTTCCAACCGTAGCTAATGCCCTCTTGTCTAATGCCACTCGTCAGTACAAGAATCGTAAAGAAACTGGGAATGGTT
CAACTTCTTGGACTCCTCCAGGTTGGCATCAGGTCAAGAATCTAAAGGGCTCTTATACCCATGCAGTCGATAGAGGTCAT
TTGTTAGGCTATGCCTTAATCGGTGGTTTGGATGGTTTTGATGCCTCAACAAGCAATCCTAAAAACATTGCTGTTCAGAC
AGCCTGGGCAAATCAGGCACAAGCCGAGTATTCGACTGGTCAAAACTACTATGAAAGCAAGGTGCGTAAAGCCTTGGACC
AAAACAAGCGTGTCCGTTACCGTGTAACCCTTTACTACGCTTCAAACGAGGATTTAGTTCCCTCAGCTTCACAGATTGAA
GCCAAGTCTTCGGATGGAGAATTGGAATTCAATGTTCTAGTTCCCAATGTTCAAAAGGGACTTCAACTGGATTACCGAAC
TGGAGAAGTAACTGTAACTCAGTAA

Domains

Predicted by InterproScan.

DNA/RNA non-specific endonuclease (Endonuclease_NS)

(75-247)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  endA Streptococcus pneumoniae Rx1

98.905

100

0.989
  endA Streptococcus pneumoniae D39

98.905

100

0.989
  endA Streptococcus pneumoniae R6

98.905

100

0.989
  endA Streptococcus pneumoniae TIGR4

98.905

100

0.989

Multiple sequence alignment