Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   ACFCQI_RS09265 Genome accession   NZ_CP170722
Coordinates   2049659..2050717 (+) Length   352 a.a.
NCBI ID   WP_395121191.1    Uniprot ID   A0AB74USK4
Organism   Rhodanobacter sp. FW102-FHT14D06     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2044659..2055717
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACFCQI_RS09250 (ACFCQI_09250) - 2044764..2046059 (+) 1296 WP_395121185.1 citrate synthase -
  ACFCQI_RS09255 (ACFCQI_09255) - 2046141..2046815 (-) 675 WP_395121187.1 hypothetical protein -
  ACFCQI_RS09260 (ACFCQI_09260) - 2046873..2049404 (-) 2532 WP_395121189.1 penicillin-binding protein 1A -
  ACFCQI_RS09265 (ACFCQI_09265) pilM 2049659..2050717 (+) 1059 WP_395121191.1 pilus assembly protein PilM Machinery gene
  ACFCQI_RS09270 (ACFCQI_09270) - 2050717..2051436 (+) 720 WP_395121193.1 PilN domain-containing protein -
  ACFCQI_RS09275 (ACFCQI_09275) - 2051433..2052083 (+) 651 WP_395121195.1 type 4a pilus biogenesis protein PilO -
  ACFCQI_RS09280 (ACFCQI_09280) - 2052083..2052625 (+) 543 WP_395121197.1 pilus assembly protein PilP -
  ACFCQI_RS09285 (ACFCQI_09285) pilQ 2052681..2054903 (+) 2223 WP_395121199.1 type IV pilus secretin PilQ -

Sequence

Protein


Download         Length: 352 a.a.        Molecular weight: 37787.35 Da        Isoelectric Point: 4.5159

>NTDB_id=1058884 ACFCQI_RS09265 WP_395121191.1 2049659..2050717(+) (pilM) [Rhodanobacter sp. FW102-FHT14D06]
MGLFTPKKPALIGVDISSTAVKLLQLSQAGGRYRVEHYAVEPLPPNAVVEKNIVEVEAVGDAIRRALARSGSKLKHAAAA
VAGSAVITRIIPMTSELSDDDLEGQIQVEANQYIPYPIDEVSLDFEVIGPVRDNPDMNNVLLAASRTENVDMRVAALDLG
GLTAGVIDVEAFAMENAFAMVADQLNVGRDALVAVVDIGATMTTLSVLRNQRTIYSREQVFGGKQLTDEIMRRFGLSYEE
AGRAKRKGGLPESYETEALEPFKESLIQQISRLLQFFFAGSEYSKVDQVVLAGGCASIEGLGPMLEEQLGVPCVVANPLA
RMSLSPRVQAQALAQDAPALMIAVGLALRSFD

Nucleotide


Download         Length: 1059 bp        

>NTDB_id=1058884 ACFCQI_RS09265 WP_395121191.1 2049659..2050717(+) (pilM) [Rhodanobacter sp. FW102-FHT14D06]
GTGGGGCTCTTTACACCGAAGAAGCCGGCGCTGATCGGCGTCGACATCAGTTCGACCGCCGTCAAGCTGCTGCAGCTGAG
TCAGGCGGGCGGGCGCTATCGCGTGGAGCACTACGCGGTGGAACCGCTCCCGCCCAATGCGGTGGTCGAGAAGAACATCG
TCGAGGTCGAGGCGGTGGGCGACGCGATCCGCCGCGCCCTGGCCCGCTCGGGTTCCAAGCTCAAGCACGCCGCCGCCGCG
GTGGCCGGGTCGGCGGTGATCACGCGGATCATCCCGATGACCAGCGAGCTATCCGACGATGACCTCGAGGGCCAGATCCA
GGTCGAGGCCAATCAGTACATCCCGTATCCGATCGACGAGGTCAGCCTCGATTTCGAGGTGATCGGCCCGGTACGCGACA
ACCCGGACATGAACAACGTGCTGCTGGCGGCCTCGCGCACCGAGAACGTCGACATGCGCGTGGCGGCGTTGGATCTGGGC
GGCCTCACTGCCGGCGTCATCGACGTGGAGGCCTTCGCGATGGAGAACGCCTTCGCGATGGTCGCCGACCAGCTCAACGT
CGGCCGCGACGCCCTGGTGGCGGTGGTCGACATCGGCGCGACCATGACCACGCTTTCCGTGCTGCGCAACCAGCGCACCA
TCTATTCGCGCGAACAGGTGTTCGGCGGCAAGCAGCTCACCGACGAGATCATGCGCCGCTTCGGGCTCTCCTACGAGGAG
GCTGGCCGCGCCAAGCGCAAGGGCGGCCTGCCGGAGTCCTACGAGACCGAGGCGCTGGAGCCGTTCAAGGAGTCGCTGAT
CCAGCAGATCAGCCGCCTGCTGCAGTTCTTCTTCGCCGGCAGCGAATACAGCAAGGTCGACCAGGTGGTGCTGGCCGGCG
GCTGCGCCTCGATCGAGGGACTGGGCCCGATGCTGGAAGAGCAGCTCGGCGTGCCTTGCGTGGTCGCCAACCCGCTGGCC
CGCATGTCGTTGTCGCCGCGGGTGCAGGCCCAGGCGCTGGCGCAGGATGCCCCGGCCCTGATGATCGCGGTCGGCCTCGC
CCTGAGGAGCTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(13-351)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

51.989

100

0.52
  comM Acinetobacter nosocomialis M2

51.705

100

0.517
  comM Acinetobacter baylyi ADP1

51.714

99.432

0.514
  pilM Legionella pneumophila strain ERS1305867

43.059

100

0.432

Multiple sequence alignment