Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   ACFXAA_RS19175 Genome accession   NZ_CP170717
Coordinates   3903710..3904780 (+) Length   356 a.a.
NCBI ID   WP_003153083.1    Uniprot ID   -
Organism   Bacillus amyloliquefaciens strain JP5014     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3898710..3909780
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACFXAA_RS19145 (ACFXAA_19145) - 3899183..3899428 (-) 246 WP_003153078.1 DUF2626 domain-containing protein -
  ACFXAA_RS19150 (ACFXAA_19150) - 3899665..3900045 (+) 381 WP_003153079.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  ACFXAA_RS19160 (ACFXAA_19160) - 3900252..3901082 (+) 831 WP_039254482.1 STAS domain-containing protein -
  ACFXAA_RS19165 (ACFXAA_19165) - 3901120..3902421 (-) 1302 WP_012117986.1 hemolysin family protein -
  ACFXAA_RS19170 (ACFXAA_19170) - 3902567..3903517 (+) 951 WP_015417820.1 magnesium transporter CorA family protein -
  ACFXAA_RS19175 (ACFXAA_19175) comGA 3903710..3904780 (+) 1071 WP_003153083.1 competence type IV pilus ATPase ComGA Machinery gene
  ACFXAA_RS19180 (ACFXAA_19180) comGB 3904767..3905804 (+) 1038 WP_395135501.1 competence type IV pilus assembly protein ComGB Machinery gene
  ACFXAA_RS19185 (ACFXAA_19185) comGC 3905851..3906117 (+) 267 WP_050515801.1 competence type IV pilus major pilin ComGC Machinery gene
  ACFXAA_RS19190 (ACFXAA_19190) comGD 3906107..3906544 (+) 438 WP_395135502.1 competence type IV pilus minor pilin ComGD Machinery gene
  ACFXAA_RS19195 (ACFXAA_19195) comGE 3906528..3906842 (+) 315 WP_094032244.1 competence type IV pilus minor pilin ComGE -
  ACFXAA_RS19200 (ACFXAA_19200) comGF 3906751..3907251 (+) 501 WP_258566475.1 competence type IV pilus minor pilin ComGF -
  ACFXAA_RS19205 (ACFXAA_19205) comGG 3907252..3907629 (+) 378 WP_015417814.1 competence type IV pilus minor pilin ComGG Machinery gene
  ACFXAA_RS19210 (ACFXAA_19210) - 3907686..3907865 (+) 180 WP_003153093.1 YqzE family protein -
  ACFXAA_RS19215 (ACFXAA_19215) - 3907905..3908234 (-) 330 WP_060674607.1 DUF3889 domain-containing protein -
  ACFXAA_RS19220 (ACFXAA_19220) tapA 3908493..3909164 (+) 672 WP_060674605.1 amyloid fiber anchoring/assembly protein TapA -
  ACFXAA_RS19225 (ACFXAA_19225) sipW 3909136..3909720 (+) 585 WP_061860711.1 signal peptidase I SipW -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40529.85 Da        Isoelectric Point: 9.4729

>NTDB_id=1058693 ACFXAA_RS19175 WP_003153083.1 3903710..3904780(+) (comGA) [Bacillus amyloliquefaciens strain JP5014]
MKNIETYSRNIINEAYTARASDIHIVPREKDAFIHFRIGHALVKKRVLKKEECVRLISHFKFLSAMDIGERRKPQNGSLS
LPLPTETVHLRMSTLPTMNDESLVIRLLPKRQIPPLDKLSLFRGAGAALLSFLKHSHGLLLFTGPTGSGKTTTLYSLVNY
AKRHFNRRIVTLEDPVETRDEDVLQVQVNEKAGVTYSAGLKAILRHDPDMIILGEIRDAETAEIAVRAAMTGHLVLSSLH
TRDAKGAIYRLLEFGVNMTEIEQTVIAIAAQRLVDLTCPFCKEENCSVYCRMYRQTRRAGIYELLYGKNLTQCFQEAKGE
HANFQYQTLRRLIRKGIALGYVTTDNYDRWVYHEAD

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=1058693 ACFXAA_RS19175 WP_003153083.1 3903710..3904780(+) (comGA) [Bacillus amyloliquefaciens strain JP5014]
GTGAAAAATATTGAAACATATAGCAGGAACATCATAAACGAGGCCTATACGGCAAGGGCTTCAGATATACACATTGTGCC
GAGAGAAAAGGATGCTTTCATTCATTTTCGCATCGGCCATGCTCTTGTGAAAAAAAGAGTCTTAAAAAAAGAGGAATGCG
TAAGGCTTATTTCTCATTTTAAGTTTTTATCGGCCATGGATATAGGAGAAAGACGTAAGCCGCAAAACGGGTCATTGTCT
CTGCCGCTTCCGACGGAAACCGTTCATTTGAGAATGTCAACCTTGCCGACAATGAATGATGAAAGTCTTGTCATCAGGCT
TTTGCCTAAGCGGCAGATTCCCCCTCTGGATAAACTGTCCTTATTTCGGGGGGCGGGCGCCGCACTATTGTCCTTTCTGA
AGCATTCGCACGGCCTGCTTCTGTTTACCGGCCCCACCGGATCTGGAAAAACGACCACTCTTTACTCGCTTGTCAACTAT
GCAAAAAGGCATTTCAACCGGCGGATTGTCACGCTTGAAGACCCCGTAGAAACAAGAGATGAGGATGTGCTGCAAGTTCA
GGTGAATGAAAAAGCCGGAGTCACATATTCCGCCGGTTTAAAAGCGATTTTAAGACATGATCCCGATATGATCATTCTAG
GGGAAATCAGAGATGCCGAGACGGCTGAAATCGCAGTCCGCGCGGCAATGACGGGACACCTCGTTCTTTCAAGCCTGCAT
ACAAGAGATGCTAAGGGCGCCATATACAGGCTGCTGGAGTTCGGCGTGAACATGACAGAAATTGAACAAACCGTCATTGC
TATTGCCGCTCAGCGTCTGGTTGATTTAACATGTCCGTTCTGCAAAGAGGAAAACTGTTCCGTGTACTGCCGGATGTACA
GGCAGACACGCCGTGCGGGCATTTATGAACTGCTCTACGGGAAAAACCTCACCCAATGCTTTCAGGAAGCAAAGGGAGAG
CACGCCAATTTTCAATATCAGACGCTCCGCAGGCTGATCAGAAAAGGAATCGCGCTCGGCTATGTCACGACCGACAATTA
CGATCGGTGGGTGTATCATGAAGCGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

82.022

100

0.82
  pilB Glaesserella parasuis strain SC1401

38.793

97.753

0.379
  pilF Thermus thermophilus HB27

37.892

98.596

0.374

Multiple sequence alignment