Detailed information    

insolico Bioinformatically predicted

Overview

Name   comE   Type   Machinery gene
Locus tag   O1Q83_RS01505 Genome accession   NZ_CP170642
Coordinates   305751..307139 (-) Length   462 a.a.
NCBI ID   WP_132302011.1    Uniprot ID   A0A4R1KYA0
Organism   Lonepinella koalarum strain ACM3666     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
IScluster/Tn 303568..305190 305751..307139 flank 561


Gene organization within MGE regions


Location: 303568..307139
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  O1Q83_RS01485 (O1Q83_00296) - 303568..303843 (+) 276 Protein_282 MerR family DNA-binding transcriptional regulator -
  O1Q83_RS01490 (O1Q83_00297) - 303875..304141 (+) 267 WP_207905239.1 helix-turn-helix domain-containing protein -
  O1Q83_RS01495 (O1Q83_00298) - 304093..305190 (+) 1098 WP_243640865.1 transposase -
  O1Q83_RS01500 (O1Q83_00299) acpP 305482..305712 (+) 231 WP_132302013.1 acyl carrier protein -
  O1Q83_RS01505 (O1Q83_00300) comE 305751..307139 (-) 1389 WP_132302011.1 type IV pilus secretin PilQ Machinery gene

Sequence

Protein


Download         Length: 462 a.a.        Molecular weight: 50690.95 Da        Isoelectric Point: 6.1224

>NTDB_id=1058276 O1Q83_RS01505 WP_132302011.1 305751..307139(-) (comE) [Lonepinella koalarum strain ACM3666]
MNLLTFILKCGVFFCFFAAYSYAESDHATFSIRLKQAPLVTTLQQLALEQNANLIINDELTGTLSLQLENTTLDQLFQAV
AKIKNLSLLKEKGIYYLTQESIPQDNALNNLPIMAEFDSVPTIQPLHTTTLKLHFAKASEVMKSLTTGNGSLLSGEGSIS
FDDRSNVLIVKDNALSIKNIRKLVKELDKPIEQIAIEARIVTITDESLQELGVRWGLFDPTSSSHKVAGSLAANGFNNIT
DNLNVNFGAVNTPAGSLALQVAKINGRLLDLELTALERENNVKIIASPKLLTTNKKSASIKQGTEIPYVSTNEKSGSQNV
EFREAVLGLEVTPHISQDNAILLDLIVSQNSPGASVQSGEGEVITIDKQEINTQVFAKDGETIVLGGVFQDTLSKGVDKV
PVLGDIPVIKHLFRKQNDKHQKRELVIFVTPHILKQGQSLQDVQKNRQKDNKITENNAKLIE

Nucleotide


Download         Length: 1389 bp        

>NTDB_id=1058276 O1Q83_RS01505 WP_132302011.1 305751..307139(-) (comE) [Lonepinella koalarum strain ACM3666]
ATGAATTTATTAACGTTTATCTTAAAGTGCGGTGTCTTTTTTTGTTTTTTTGCTGCTTATAGCTATGCTGAATCAGATCA
TGCCACTTTTTCTATTCGCTTAAAACAGGCGCCATTAGTTACCACGTTACAACAGCTTGCGTTAGAACAAAATGCAAATT
TAATCATTAATGATGAATTAACTGGAACATTATCATTACAATTAGAAAACACTACGCTTGATCAGCTTTTTCAAGCCGTC
GCAAAAATTAAAAATCTGTCGTTGCTCAAAGAAAAAGGAATCTATTATTTGACTCAAGAGAGTATTCCACAAGACAATGC
GTTAAACAATTTACCGATAATGGCTGAATTTGATTCTGTACCTACAATACAACCATTACATACCACCACGCTAAAATTGC
ATTTCGCCAAAGCCTCCGAAGTGATGAAATCTTTAACCACTGGGAATGGATCTTTATTATCAGGGGAAGGTAGCATTAGC
TTTGATGATCGCAGTAATGTATTAATCGTGAAAGACAATGCTCTGTCGATTAAAAATATTCGTAAGCTGGTCAAAGAGTT
GGACAAACCAATAGAACAAATTGCGATTGAAGCGCGCATTGTTACCATTACAGATGAAAGTTTACAAGAATTAGGGGTTC
GTTGGGGATTATTCGATCCGACAAGCAGTTCCCATAAAGTGGCAGGAAGCCTTGCTGCCAATGGGTTTAATAATATCACT
GATAATTTAAATGTGAATTTCGGCGCAGTCAATACCCCTGCTGGTTCGTTGGCATTACAAGTGGCCAAAATTAATGGTCG
ATTATTAGACCTAGAATTAACCGCACTTGAGCGAGAAAATAACGTCAAAATTATTGCTAGCCCCAAATTACTTACCACTA
ACAAAAAATCAGCGAGTATTAAACAAGGTACTGAAATCCCTTATGTATCGACCAACGAAAAAAGCGGTTCGCAAAATGTA
GAATTTCGTGAAGCCGTATTAGGCTTAGAGGTAACACCCCATATTTCACAAGATAATGCCATTTTGTTGGATCTTATTGT
CAGCCAAAATTCCCCAGGAGCTTCGGTACAATCCGGCGAGGGAGAAGTAATCACCATTGATAAACAAGAAATTAACACGC
AAGTTTTTGCCAAAGACGGTGAAACTATTGTATTAGGTGGGGTATTTCAAGATACATTAAGTAAAGGAGTCGATAAAGTC
CCCGTATTAGGTGATATTCCTGTGATCAAACATCTATTCCGTAAACAAAATGATAAACACCAAAAACGCGAATTAGTGAT
TTTTGTGACACCACATATTTTAAAACAAGGACAATCTCTTCAAGATGTTCAGAAAAACCGTCAGAAAGACAACAAAATTA
CTGAAAACAACGCAAAGTTAATTGAATAG

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(275-435)

Bacterial type II/III secretion system short domain (Secretin_N)

(130-193)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A4R1KYA0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comE Haemophilus influenzae Rd KW20

63.393

96.97

0.615
  comE Haemophilus influenzae 86-028NP

62.921

96.32

0.606
  comE Glaesserella parasuis strain SC1401

54.481

91.775

0.5
  pilQ Vibrio campbellii strain DS40M4

40.515

92.424

0.374
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

39.535

93.074

0.368
  pilQ Vibrio cholerae strain A1552

39.535

93.074

0.368

Multiple sequence alignment