Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF   Type   Machinery gene
Locus tag   ACFIOQ_RS06360 Genome accession   NZ_CP170404
Coordinates   1222736..1223233 (+) Length   165 a.a.
NCBI ID   WP_001825340.1    Uniprot ID   A0A2S6DDC2
Organism   Staphylococcus aureus strain t7960     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1217736..1228233
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACFIOQ_RS06320 - 1217780..1218766 (+) 987 WP_000161315.1 ROK family glucokinase -
  ACFIOQ_RS06325 - 1218766..1219095 (+) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  ACFIOQ_RS06330 - 1219092..1219715 (+) 624 WP_001223008.1 MBL fold metallo-hydrolase -
  ACFIOQ_RS06335 comGA 1219767..1220741 (+) 975 WP_111063875.1 competence type IV pilus ATPase ComGA Machinery gene
  ACFIOQ_RS06340 comGB 1220713..1221783 (+) 1071 WP_000776413.1 competence type IV pilus assembly protein ComGB Machinery gene
  ACFIOQ_RS06345 comGC 1221797..1222108 (+) 312 WP_000472256.1 competence type IV pilus major pilin ComGC Machinery gene
  ACFIOQ_RS06350 comGD 1222086..1222532 (+) 447 WP_072357807.1 competence type IV pilus minor pilin ComGD Machinery gene
  ACFIOQ_RS06355 comGE 1222519..1222818 (+) 300 WP_000844415.1 hypothetical protein Machinery gene
  ACFIOQ_RS06360 comGF 1222736..1223233 (+) 498 WP_001825340.1 competence type IV pilus minor pilin ComGF Machinery gene
  ACFIOQ_RS06365 - 1223330..1223476 (+) 147 WP_001789879.1 hypothetical protein -
  ACFIOQ_RS06370 - 1223466..1223990 (+) 525 WP_001015120.1 shikimate kinase -
  ACFIOQ_RS06375 gcvT 1224149..1225240 (+) 1092 WP_000093354.1 glycine cleavage system aminomethyltransferase GcvT -
  ACFIOQ_RS06380 gcvPA 1225260..1226606 (+) 1347 WP_000019687.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPA -
  ACFIOQ_RS06385 gcvPB 1226599..1228071 (+) 1473 WP_000202175.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPB -

Sequence

Protein


Download         Length: 165 a.a.        Molecular weight: 19179.92 Da        Isoelectric Point: 10.2192

>NTDB_id=1056815 ACFIOQ_RS06360 WP_001825340.1 1222736..1223233(+) (comGF) [Staphylococcus aureus strain t7960]
MILSKVTNKFVLFQKIPLLIKRHVYSINVKAFSLIEMLVAMMVISITLLIVPDLIRLSKTFLIESRELTTVDFEFFSRDI
LEDFKGVDRNDIEIRQQRIILHKGEEMIEYKLINNKIIKVVNDRGNITLINNVTAFTANIYYKSIIKITITVKVGTNLQT
KTIYV

Nucleotide


Download         Length: 498 bp        

>NTDB_id=1056815 ACFIOQ_RS06360 WP_001825340.1 1222736..1223233(+) (comGF) [Staphylococcus aureus strain t7960]
ATGATATTAAGCAAAGTGACCAACAAATTTGTGCTATTTCAAAAAATACCACTTCTTATCAAAAGACATGTATACAGTAT
TAATGTCAAAGCTTTTTCGCTTATTGAAATGTTAGTAGCGATGATGGTTATAAGTATAACTTTACTAATTGTTCCAGACT
TAATTAGACTTAGCAAAACTTTTCTAATTGAAAGTAGGGAATTAACAACTGTAGATTTCGAATTTTTCTCAAGAGATATT
CTAGAGGATTTTAAAGGAGTAGATAGAAACGATATTGAAATTAGGCAACAGCGTATCATTTTACATAAAGGTGAAGAAAT
GATCGAATACAAATTAATAAATAATAAAATTATTAAAGTTGTAAATGACAGAGGAAATATAACATTGATTAATAATGTTA
CAGCATTTACTGCAAATATCTACTATAAATCTATTATTAAAATAACGATAACAGTTAAAGTCGGTACAAATCTGCAGACT
AAAACTATTTATGTATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(28-51)

Putative Competence protein ComGF (ComGF)

(68-155)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2S6DDC2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF Staphylococcus aureus N315

98.182

100

0.982
  comGF Staphylococcus aureus MW2

96.97

100

0.97

Multiple sequence alignment