Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   O1Q79_RS06390 Genome accession   NZ_CP170386
Coordinates   1323438..1323911 (-) Length   157 a.a.
NCBI ID   WP_386690997.1    Uniprot ID   -
Organism   Lonepinella sp. MS14434     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1318438..1328911
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  O1Q79_RS06360 (O1Q79_01273) - 1318999..1319265 (-) 267 WP_386690985.1 GNAT family N-acetyltransferase -
  O1Q79_RS06365 (O1Q79_01274) yacG 1319268..1319468 (-) 201 WP_132301902.1 DNA gyrase inhibitor YacG -
  O1Q79_RS06370 (O1Q79_01275) coaE 1319452..1320081 (-) 630 WP_386690988.1 dephospho-CoA kinase -
  O1Q79_RS06375 (O1Q79_01276) - 1320107..1320769 (-) 663 WP_386690991.1 prepilin peptidase -
  O1Q79_RS06380 (O1Q79_01277) pilC 1320802..1322022 (-) 1221 WP_386690993.1 type II secretion system F family protein Machinery gene
  O1Q79_RS06385 (O1Q79_01278) pilB 1322032..1323435 (-) 1404 WP_386690995.1 GspE/PulE family protein Machinery gene
  O1Q79_RS06390 (O1Q79_01279) pilA 1323438..1323911 (-) 474 WP_386690997.1 prepilin peptidase-dependent pilin Machinery gene
  O1Q79_RS06395 (O1Q79_01280) ampD 1324035..1324580 (+) 546 WP_386690999.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  O1Q79_RS06400 (O1Q79_01281) - 1324584..1325234 (+) 651 WP_386691001.1 HAD family hydrolase -
  O1Q79_RS06405 (O1Q79_01282) bamE 1325313..1325729 (-) 417 WP_386691003.1 outer membrane protein assembly factor BamE -
  O1Q79_RS06410 (O1Q79_01283) yejK 1325792..1326811 (-) 1020 WP_386691005.1 nucleoid-associated protein YejK -
  O1Q79_RS06415 (O1Q79_01284) - 1326922..1327143 (+) 222 WP_386690281.1 YejL family protein -

Sequence

Protein


Download         Length: 157 a.a.        Molecular weight: 16446.49 Da        Isoelectric Point: 8.0999

>NTDB_id=1056521 O1Q79_RS06390 WP_386690997.1 1323438..1323911(-) (pilA) [Lonepinella sp. MS14434]
MNFSYTSHNISFKKGFTLIELMIVIAIVAILATIAIPSYQNYTKKAAISELLQAAAPYRSEVELCVYNQDAVKNCSGGQN
GIQANTTTAKGKYVKSVSVANGVITVTGNGSVSDVSYTLTASGSAKEGVTWTANCGSNTELFPSGFCSTSTNTSTSN

Nucleotide


Download         Length: 474 bp        

>NTDB_id=1056521 O1Q79_RS06390 WP_386690997.1 1323438..1323911(-) (pilA) [Lonepinella sp. MS14434]
ATGAATTTTAGCTACACTTCACACAATATTTCATTCAAAAAAGGTTTTACCCTCATTGAATTAATGATCGTCATCGCTAT
CGTCGCCATTTTAGCCACTATTGCTATTCCGTCTTATCAAAATTATACCAAAAAAGCGGCTATTTCTGAATTATTGCAGG
CTGCTGCGCCCTATCGTTCGGAAGTTGAACTTTGTGTATATAATCAAGATGCTGTTAAGAATTGCTCAGGTGGTCAAAAT
GGTATTCAAGCGAATACTACAACAGCAAAAGGCAAATATGTAAAATCTGTTTCTGTGGCGAATGGGGTAATTACGGTGAC
CGGTAATGGTAGTGTAAGTGATGTAAGTTACACATTAACGGCTAGTGGTTCTGCTAAAGAGGGCGTTACTTGGACGGCAA
ACTGTGGTTCAAATACTGAATTATTCCCATCGGGTTTTTGTAGCACAAGCACGAATACTAGCACGAGTAACTAG

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(13-35)

Pilin (bacterial filament) (Pilin)

(45-145)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

64.336

91.083

0.586
  pilA Haemophilus influenzae Rd KW20

62.238

91.083

0.567
  pilA Glaesserella parasuis strain SC1401

54.348

87.898

0.478

Multiple sequence alignment