Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   ACFDAU_RS02865 Genome accession   NZ_CP170197
Coordinates   580383..581249 (+) Length   288 a.a.
NCBI ID   WP_413438557.1    Uniprot ID   -
Organism   Sulfuriferula sp. GW1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 575383..586249
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACFDAU_RS02850 (ACFDAU_02850) - 575802..577091 (+) 1290 WP_413438554.1 HlyC/CorC family transporter -
  ACFDAU_RS02855 (ACFDAU_02855) pilB 577179..578894 (+) 1716 WP_413438555.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACFDAU_RS02860 (ACFDAU_02860) pilC 579120..580349 (+) 1230 WP_413438556.1 type II secretion system F family protein Machinery gene
  ACFDAU_RS02865 (ACFDAU_02865) pilD 580383..581249 (+) 867 WP_413438557.1 prepilin peptidase Machinery gene
  ACFDAU_RS02870 (ACFDAU_02870) coaE 581252..581854 (+) 603 WP_413438558.1 dephospho-CoA kinase -
  ACFDAU_RS02875 (ACFDAU_02875) zapD 582036..582788 (+) 753 WP_413438559.1 cell division protein ZapD -
  ACFDAU_RS02880 (ACFDAU_02880) yacG 582785..582979 (+) 195 WP_413439489.1 DNA gyrase inhibitor YacG -
  ACFDAU_RS02885 (ACFDAU_02885) rpiA 582976..583635 (-) 660 WP_413439490.1 ribose-5-phosphate isomerase RpiA -
  ACFDAU_RS02890 (ACFDAU_02890) ilvA 583752..585278 (+) 1527 WP_413438560.1 threonine ammonia-lyase, biosynthetic -

Sequence

Protein


Download         Length: 288 a.a.        Molecular weight: 30777.83 Da        Isoelectric Point: 7.6152

>NTDB_id=1055497 ACFDAU_RS02865 WP_413438557.1 580383..581249(+) (pilD) [Sulfuriferula sp. GW1]
MPVSFAALLSADPQLFALLCGGFGLLIGSFLNVVIHRLPKIMERDWGAQCAALRGEDAPSAAPYNLMVPRSACTSCGHLI
GALENIPVLSYAWLRGKCAGCGAHISLRYPLVEALTGVLSAFAAWHFGFGWAAAGALLLVWMLIALTFIDFDTQLLPDDL
TLPLLWLGLLFNLGGVFVPLDQAVVGAIVGYMVLWSIYWLFKLITGKEGMGYGDFKLLAAIGAWLGWKMLPLVILLSSFI
GALVGIVLIVLARRGRSVPIPFGPYLAGGGLVALFWGAPILQAYLAGF

Nucleotide


Download         Length: 867 bp        

>NTDB_id=1055497 ACFDAU_RS02865 WP_413438557.1 580383..581249(+) (pilD) [Sulfuriferula sp. GW1]
ATGCCTGTGTCCTTTGCTGCGCTGTTGAGCGCAGACCCGCAGCTGTTTGCCCTACTCTGTGGCGGTTTTGGCCTGTTGAT
CGGCAGCTTTCTGAATGTGGTCATCCACCGCCTGCCAAAAATCATGGAACGCGACTGGGGTGCCCAGTGCGCTGCGCTGA
GGGGGGAAGATGCGCCGTCAGCGGCTCCATATAATCTGATGGTGCCGCGCTCCGCCTGCACGAGTTGCGGCCACCTTATC
GGGGCTCTGGAAAACATTCCGGTGCTCAGCTATGCCTGGTTGCGCGGAAAGTGCGCTGGTTGCGGCGCGCATATCAGTCT
GCGTTACCCGTTGGTGGAAGCGCTTACCGGGGTGCTTTCTGCATTTGCAGCCTGGCATTTCGGTTTTGGCTGGGCAGCAG
CCGGTGCTTTGCTTTTGGTATGGATGCTGATCGCGCTGACCTTTATTGACTTCGATACCCAGCTTTTGCCGGACGACTTG
ACGTTGCCTTTATTGTGGCTGGGACTGCTGTTCAACCTCGGTGGCGTATTTGTGCCGCTGGATCAAGCAGTCGTCGGAGC
CATCGTGGGCTATATGGTGCTATGGAGCATTTATTGGCTGTTCAAGCTGATCACCGGTAAAGAGGGTATGGGTTATGGCG
ATTTCAAATTGCTTGCCGCCATCGGCGCCTGGCTGGGTTGGAAGATGCTGCCTTTGGTTATCCTGCTGTCCTCCTTTATT
GGCGCTCTGGTCGGCATTGTGCTGATTGTGCTGGCGCGACGCGGGCGTTCCGTGCCGATTCCGTTTGGACCTTATCTGGC
GGGCGGTGGACTGGTTGCTCTGTTTTGGGGCGCGCCCATCTTGCAGGCCTATCTGGCCGGGTTTTAA

Domains

Predicted by InterproScan.

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(23-127)

Type IV leader peptidase family (Peptidase_A24)

(137-246)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

54.513

96.181

0.524
  pilD Vibrio campbellii strain DS40M4

52.878

96.528

0.51
  pilD Acinetobacter baumannii D1279779

50.752

92.361

0.469
  pilD Neisseria gonorrhoeae MS11

53.386

87.153

0.465
  pilD Acinetobacter nosocomialis M2

50

92.361

0.462

Multiple sequence alignment