Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACFDAU_RS02855 Genome accession   NZ_CP170197
Coordinates   577179..578894 (+) Length   571 a.a.
NCBI ID   WP_413438555.1    Uniprot ID   -
Organism   Sulfuriferula sp. GW1     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 572179..583894
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACFDAU_RS02830 (ACFDAU_02830) rimM 572675..573190 (-) 516 WP_413438551.1 ribosome maturation factor RimM -
  ACFDAU_RS02835 (ACFDAU_02835) rpsP 573165..573419 (-) 255 WP_147070771.1 30S ribosomal protein S16 -
  ACFDAU_RS02840 (ACFDAU_02840) ffh 573503..574846 (-) 1344 WP_413438552.1 signal recognition particle protein -
  ACFDAU_RS02845 (ACFDAU_02845) - 574923..575729 (+) 807 WP_413438553.1 inner membrane protein YpjD -
  ACFDAU_RS02850 (ACFDAU_02850) - 575802..577091 (+) 1290 WP_413438554.1 HlyC/CorC family transporter -
  ACFDAU_RS02855 (ACFDAU_02855) pilB 577179..578894 (+) 1716 WP_413438555.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACFDAU_RS02860 (ACFDAU_02860) pilC 579120..580349 (+) 1230 WP_413438556.1 type II secretion system F family protein Machinery gene
  ACFDAU_RS02865 (ACFDAU_02865) pilD 580383..581249 (+) 867 WP_413438557.1 prepilin peptidase Machinery gene
  ACFDAU_RS02870 (ACFDAU_02870) coaE 581252..581854 (+) 603 WP_413438558.1 dephospho-CoA kinase -
  ACFDAU_RS02875 (ACFDAU_02875) zapD 582036..582788 (+) 753 WP_413438559.1 cell division protein ZapD -
  ACFDAU_RS02880 (ACFDAU_02880) yacG 582785..582979 (+) 195 WP_413439489.1 DNA gyrase inhibitor YacG -
  ACFDAU_RS02885 (ACFDAU_02885) rpiA 582976..583635 (-) 660 WP_413439490.1 ribose-5-phosphate isomerase RpiA -

Sequence

Protein


Download         Length: 571 a.a.        Molecular weight: 62588.99 Da        Isoelectric Point: 4.9318

>NTDB_id=1055495 ACFDAU_RS02855 WP_413438555.1 577179..578894(+) (pilB) [Sulfuriferula sp. GW1]
MAATIANSGLTGLARALVQEKWLTESEAEALAKQATSSGEGFVSQVISVKKIKPVQIAEFAAAQFGFPYFDLLALDPELL
PKSLLDPKLAQKRRVLALYQRGNRLSVAVSDPTNLQAIDEVKFQTNLGVDIVVVEDDKLGKLIEQLSEGSASVLDNLDSE
DIDLEFTDDEALAKVDDAASMDIDDAPIVRYLQKILLDAINTGVSDIHFEPFEKFYRIRYRLDGQLYEVAQPPIAIKEKI
SSRIKVISKLDIAEKRVPQDGRMKLVLSKNRAIDFRVSSLPTLYGEKIVMRILDPASAQLGIDALGYDPDQKELLLNAVQ
RPYGMVLVTGPTGSGKTVSLYTCLNILNKPGVNISTAEDPAEINLPGINQVNVNDKAGLTFSVALRAFLRQDPDVIMVGE
IRDLETADISIKAAQTGHMVLSTLHTNDAPTTLTRLMNMGVAPFNIASSVILITAQRLARRLCSCKQPATIPKEALLRAG
FREEDLDGNWQPYHAVGCELCKNTGYKGRVGIYQVMPITDEMTRIIMKNGNAIDIADQARREGVRDLRQAGLLKVKAGMT
SLEEVEAVTNE

Nucleotide


Download         Length: 1716 bp        

>NTDB_id=1055495 ACFDAU_RS02855 WP_413438555.1 577179..578894(+) (pilB) [Sulfuriferula sp. GW1]
ATGGCAGCTACCATAGCAAATTCCGGTTTGACTGGTTTGGCGCGTGCGCTGGTCCAAGAAAAATGGCTCACTGAGTCTGA
GGCAGAGGCATTGGCCAAGCAGGCGACGAGTTCGGGAGAAGGTTTTGTTTCCCAGGTAATTAGCGTCAAAAAAATCAAAC
CAGTGCAAATTGCGGAATTTGCGGCAGCACAATTCGGTTTCCCGTATTTTGATCTATTGGCGCTTGATCCAGAATTGTTA
CCGAAAAGCTTGCTGGATCCCAAGCTGGCGCAAAAACGCAGGGTACTAGCCCTTTATCAACGAGGTAACCGTCTCTCGGT
TGCGGTGTCCGATCCTACCAATCTCCAGGCTATAGATGAGGTCAAGTTTCAGACCAATCTCGGTGTGGATATCGTGGTGG
TGGAGGATGACAAACTCGGCAAGCTTATCGAACAGCTCAGCGAGGGAAGCGCCAGCGTACTGGATAATCTCGATAGCGAA
GATATCGATCTGGAGTTTACCGACGATGAAGCGCTGGCCAAGGTGGATGATGCGGCCAGTATGGATATCGACGATGCGCC
TATCGTTCGTTACCTGCAAAAAATCCTGCTGGATGCGATCAATACCGGTGTATCTGATATCCACTTTGAGCCATTCGAGA
AATTTTATCGTATCCGATACCGTCTTGATGGGCAGTTGTACGAAGTTGCCCAGCCGCCAATTGCCATCAAGGAAAAAATC
TCCTCGCGCATCAAGGTGATCTCCAAGCTGGATATTGCCGAAAAGCGCGTACCACAGGACGGGCGCATGAAGCTGGTGCT
CTCCAAGAATCGCGCTATTGATTTTCGGGTGAGTTCATTGCCCACGCTGTATGGCGAAAAAATCGTGATGCGTATTCTTG
ATCCGGCCAGTGCGCAGCTCGGTATTGATGCGCTGGGTTATGACCCGGATCAAAAAGAGTTATTGCTTAACGCCGTACAA
CGCCCCTATGGCATGGTGCTGGTCACCGGCCCCACCGGTAGCGGTAAAACCGTGTCGTTGTATACATGCCTGAATATTCT
CAACAAGCCTGGGGTTAATATCTCCACGGCTGAGGATCCGGCCGAAATAAATTTGCCTGGTATCAATCAGGTCAACGTCA
ACGACAAGGCAGGACTGACCTTTTCTGTGGCACTGCGTGCTTTCCTGCGCCAGGATCCGGACGTCATCATGGTGGGCGAG
ATTCGTGATCTGGAAACCGCAGACATTTCCATCAAGGCCGCGCAAACCGGGCATATGGTGTTATCGACCTTGCATACCAA
TGATGCGCCCACTACGCTCACTCGGCTGATGAACATGGGTGTGGCGCCATTCAATATCGCTTCCAGTGTGATCCTGATTA
CTGCCCAGCGCCTGGCGCGACGACTTTGCAGTTGCAAACAGCCAGCGACCATTCCCAAGGAAGCGTTGCTACGCGCCGGC
TTCAGGGAGGAAGACCTGGACGGGAACTGGCAGCCGTACCATGCGGTGGGTTGCGAGCTATGCAAAAATACCGGTTACAA
AGGTCGGGTAGGTATCTATCAGGTTATGCCGATTACTGACGAGATGACCCGCATCATCATGAAAAACGGGAATGCCATCG
ATATTGCCGATCAGGCGCGGCGTGAGGGAGTCCGCGATTTGCGCCAGGCCGGGCTGCTCAAGGTCAAGGCCGGTATGACT
TCCCTGGAAGAAGTTGAAGCAGTCACCAATGAATAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(17-149)

Type II/IV secretion system protein (T2SSE)

(194-461)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.497

100

0.557
  pilB Acinetobacter baylyi ADP1

54.704

100

0.55
  pilF Neisseria gonorrhoeae MS11

53.792

99.299

0.534
  pilB Legionella pneumophila strain ERS1305867

52.686

100

0.532
  pilB Vibrio cholerae strain A1552

47.368

99.825

0.473
  pilB Vibrio parahaemolyticus RIMD 2210633

47.266

99.299

0.469
  pilB Vibrio campbellii strain DS40M4

46.831

99.475

0.466
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.392

95.622

0.396
  pilF Thermus thermophilus HB27

40.29

96.497

0.389
  pilB/pilB1 Synechocystis sp. PCC 6803

41.517

87.741

0.364

Multiple sequence alignment