Detailed information    

insolico Bioinformatically predicted

Overview


Name   ssb   Type   Machinery gene
Locus tag   BCU67_RS15020 Genome accession   NZ_CP170019
Coordinates   3319159..3319713 (-) Length   184 a.a.
NCBI ID   WP_010434324.1    Uniprot ID   A0A7Z1MML7
Organism   Vibrio cyclitrophicus strain 10N.286.49.B11     
Function   ssDNA binding (predicted from homology)   
DNA processing

Related MGE


Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 3303891..3318997 3319159..3319713 flank 162


Gene organization within MGE regions


Location: 3303891..3319713
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BCU67_RS14940 (BCU67_14935) - 3303891..3304316 (-) 426 WP_016788312.1 hypothetical protein -
  BCU67_RS14945 (BCU67_14940) - 3304288..3305055 (-) 768 WP_016795709.1 PilW family protein -
  BCU67_RS14950 (BCU67_14945) - 3305055..3305660 (-) 606 WP_016794286.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  BCU67_RS14955 (BCU67_14950) - 3305650..3306150 (-) 501 WP_102277581.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  BCU67_RS14960 (BCU67_14955) - 3306292..3306768 (-) 477 WP_102277580.1 type II secretion system protein -
  BCU67_RS14965 (BCU67_14960) - 3306804..3307379 (-) 576 WP_010434354.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  BCU67_RS14970 (BCU67_14965) - 3307459..3307914 (-) 456 WP_016790950.1 hypothetical protein -
  BCU67_RS14975 (BCU67_14970) - 3307918..3309141 (-) 1224 WP_016795710.1 type II secretion system F family protein -
  BCU67_RS14980 (BCU67_14975) - 3309154..3310878 (-) 1725 WP_010434345.1 GspE/PulE family protein -
  BCU67_RS14985 (BCU67_14980) - 3310868..3312016 (-) 1149 WP_010434341.1 tetratricopeptide repeat protein -
  BCU67_RS14990 (BCU67_14985) - 3312019..3312858 (-) 840 WP_010434338.1 ExeA family protein -
  BCU67_RS14995 (BCU67_14990) mshL 3312912..3314549 (-) 1638 WP_102277579.1 pilus (MSHA type) biogenesis protein MshL -
  BCU67_RS15000 (BCU67_14995) - 3314565..3314897 (-) 333 WP_016788320.1 MSHA biogenesis protein MshK -
  BCU67_RS15005 (BCU67_15000) pilO 3314890..3315537 (-) 648 WP_010434330.1 type 4a pilus biogenesis protein PilO -
  BCU67_RS15010 (BCU67_15005) - 3315537..3316979 (-) 1443 WP_010434328.1 MSHA biogenesis protein MshI -
  BCU67_RS15015 (BCU67_15010) csrD 3316982..3318997 (-) 2016 WP_016767796.1 RNase E specificity factor CsrD -
  BCU67_RS15020 (BCU67_15015) ssb 3319159..3319713 (-) 555 WP_010434324.1 single-stranded DNA-binding protein Machinery gene

Sequence


Protein


Download         Length: 184 a.a.        Molecular weight: 20286.42 Da        Isoelectric Point: 5.2358

>NTDB_id=1054670 BCU67_RS15020 WP_010434324.1 3319159..3319713(-) (ssb) [Vibrio cyclitrophicus strain 10N.286.49.B11]
MASRGVNKVILVGNLGNDPEIRYMPNGGAVANITIATSESWRDKATGEQREKTEWHRVALFGKLAEVAGEYLRKGSQVYI
EGQLQTRKWQDQSGQDRYTTEVVVQGFNGVMQMLGGRAQGGAPAQGGMGNQQQGGWGQPQQPQQQQQQYSAPAQQQPKAP
QQAPQQAQPQYNEPPMDFDDDIPF

Nucleotide


Download         Length: 555 bp        

>NTDB_id=1054670 BCU67_RS15020 WP_010434324.1 3319159..3319713(-) (ssb) [Vibrio cyclitrophicus strain 10N.286.49.B11]
ATGGCTAGCCGTGGAGTTAACAAAGTTATATTAGTGGGTAACCTAGGTAATGACCCTGAAATTCGTTACATGCCAAATGG
CGGCGCAGTAGCGAACATTACCATTGCAACGTCAGAGTCATGGCGTGACAAAGCAACTGGCGAACAGCGTGAAAAAACAG
AATGGCACCGTGTTGCTCTGTTTGGCAAGCTGGCGGAAGTTGCTGGTGAGTACCTACGTAAAGGTTCTCAAGTTTACATT
GAAGGTCAACTTCAGACTCGTAAATGGCAAGATCAAAGCGGTCAAGATCGCTACACAACTGAAGTGGTTGTTCAAGGCTT
CAACGGTGTAATGCAAATGCTTGGTGGCCGTGCTCAAGGCGGTGCTCCTGCTCAAGGTGGTATGGGTAACCAACAGCAAG
GTGGTTGGGGTCAACCACAGCAACCACAACAGCAGCAACAACAATACAGTGCTCCTGCTCAACAGCAGCCGAAAGCACCT
CAACAAGCTCCTCAGCAGGCTCAACCTCAATATAATGAGCCACCGATGGATTTTGATGATGACATCCCATTTTAG


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure
  AlphaFold DB A0A7Z1MML7

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ssb Vibrio cholerae strain A1552

81.72

100

0.826

  ssb Glaesserella parasuis strain SC1401

57.219

100

0.582

  ssb Neisseria meningitidis MC58

47.826

100

0.478

  ssb Neisseria gonorrhoeae MS11

47.826

100

0.478


Multiple sequence alignment